Laustendinesen7132
Despite advances in the treatment of adult T-cell leukemia/lymphoma (ATLL), the survival rate of this malignancy remains significantly low. Auraptene (AUR) is a natural coumarin with broad-spectrum anticancer activities. To introduce a more effective therapeutic strategy for ATLL, we investigated the combinatorial effects of AUR and arsenic trioxide (ATO) on MT-2 cells.
The cells were treated with different concentrations of AUR for 24, 48, and 72 hr, and viability was measured by alamarBlue assay. Then, the combination of AUR (20 μg/ml) and ATO (3 μg/ml) was administrated and the cell cycle was analyzed by PI staining followed by flow cytometry analysis. In addition, the expression of
, and
was evaluated via qPCR.
Assessment of cell viability revealed increased toxicity of AUR and ATO when used in combination. Our findings were confirmed by accumulation of cells in the sub G1 phase of the cell cycle and significant down-regulation of
.
Obtained findings suggest that combinatorial use of AUR and ATO could be considered for designing novel chemotherapy regimens for ATLL.
Obtained findings suggest that combinatorial use of AUR and ATO could be considered for designing novel chemotherapy regimens for ATLL.
The present study aimed to determine whether bone marrow mesenchymal stem cell-derived microvesicles (MSC MVs) were effective in restoring lung tissue structure, and to assess the potential role of miRNAs in the pathogenesis and progression of acute respiratory distress syndrome (ARDS).
ARDS was induced by lipopolysaccharide in male C57BL/6 mice. The degree of lung injury was assessed by histological analysis, lung's wet weight/body weight, and protein levels in the bronchoalveolar lavage fluid (BALF). Sequencing was performed on the BGISEQ-500 platform. Differentially expressed miRNAs (DEMs) were screened with the DEGseq software. The target genes of DEMs were predicted by iRNAhybrid, miRanda, and TargetScan.
Compared with LPS-injured mice, MSC MVs reduced lung water and total protein levels in the BALF, demonstrating a protective effect. 52 miRNAs were differentially expressed following treatment with MSC MVs in ARDS mice. Among them, miR-532-5p, miR-223-3p, and miR-744-5p were significantly regulated. Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed the target genes were mainly located in the cell, organelle, and membrane. Furthermore, KEGG pathways such as ErbB, PI3K-Akt, Ras, MAPK, Toll, and Wnt signaling pathways were the most significant pathways enriched by the target genes.
MSC MVs treatment was involved in alleviating lung injury and promoting lung tissue repair by dysregulated miRNAs.
MSC MVs treatment was involved in alleviating lung injury and promoting lung tissue repair by dysregulated miRNAs.
This study aimed to assess the oral health knowledge, attitude, and performance of the parents of autistic 3-12-year-old.
This descriptive cross-sectional study evaluated 50 parents of autistic 3-12-year-old in Isfahan city. The oral health knowledge, attitude, and performance of the parents were evaluated using a self-administered questionnaire with closed questions, after confirming its validity and reliability. Data were analyzed using independent
-test, one-way ANOVA, Pearson's correlation coefficient, regression analysis, and Cronbach's alpha (α =0.05).
Of children, 32 (64%) were males and 18 (36%) were females. A total of 28 (56%) fathers and 22 (44%) mothers participated in this study. The mean age of the children and parents was 8.12 ± 2.4 years and 39.6 ± 6.8 years, respectively. The mean age at the time of diagnosis of autism was 4.08 ± 1.33 years. Of all children, 24 (46.2%) had a previous dental history. The mean scores of the knowledge and attitude of the parents were 5.2 ± 1.7 out of 11, and 16.1 ± 3.1 out of 30, respectively. Of demographic variables, order of child birth had a significant correlation with attitude of the parents, and those with one single child had significantly higher attitude score (
= 0.03).
The oral health knowledge, attitude, and performance of the parents of autistic children in Isfahan city were lower than expected, which calls for knowledge enhancement strategies in this respect.
The oral health knowledge, attitude, and performance of the parents of autistic children in Isfahan city were lower than expected, which calls for knowledge enhancement strategies in this respect.
During the first experiences of clinical treatment, dental students face various challenges. The purpose of the study is to standardize the questionnaire as a suitable tool for quantitative and qualitative evaluation of psychosocial impact, stress levels, and learning effect of dental students during the transition from preclinical to clinical education in Persian.
In this cross-sectional study a questionnaire including three areas of psychosocial impact, perceived stress, and learning effect was systematically translated. In the group of experts, face validity was examined. Then, the content validity ratio (CVR) and the content validity index (CVI) were determined. The reliability of the questionnaire was determined by the test-retest method and the Cohen's Kappa coefficient. In a cross-sectional study, the psychosocial impact, stress levels, and learning effect of all students newly admitted to the endodontic, prosthetics, periodontics, and restorative dentistry departments of Shahid Beheshti Dental Schunicate well with the patient is thought to reduce stress.
This study provided a standardized tool in Persian to evaluate the psychosocial impacts, perceived stress, and learning effect of dental students during the transition to clinical education and focused on the first clinical treatment experiences. One important psychosocial factor was collegiality between teachers and students. The ability to communicate well with the patient is thought to reduce stress.
This study aimed to compare the antimicrobial effects of green tea, microwaving, cold boiled water, and chlorhexidine (CHX) on
and
on silicone pacifiers.
In this
experimental study, 60 equal-size samples of silicone pacifiers were cut, ultraviolet sterilized, and randomly divided into two groups (
= 30) for immersion in 0.5 McFarland standard suspension of
and
. The samples in each group were then randomly divided into five subgroups (
= 6) for disinfection with 0.12% CHX, cold boiled water, green tea, microwaving for 7 min, and distilled water. The sample suspensions were cultured on blood agar (for
) and Sabouraud dextrose agar (for
) and incubated. Transferase inhibitor The number of colonies was counted after 24 and 48 h. Data were analyzed using the Kruskal-Wallis and Mann-Whitney tests (
< 0.05).
At 24 and 48 h, the
colony count was the lowest in CHX and green tea subgroups followed by microwave, cold boiled water, and distilled water subgroups (
< 0.05).
CHX and green tea can significantly decrease the
and
colony count on silicone pacifiers.
CHX and green tea can significantly decrease the S. mutans and C. albicans colony count on silicone pacifiers.
Small chipping or fracture of ceramic restorations may be repaired by composite resin instead of replacing the restoration. This method is faster and cheaper compared to restoration replacement. Several strategies have been suggested to obtain a high repair shear bond strength (SBS). This study aimed to assess the efficacy of some new ceramic surface treatments (laser and universal adhesive) to enhance the repair bond strength of composite resin to ceramic compared to the conventional method.
This
study evaluated 80 IPS Empress Esthetic ceramic plates in eight groups (
= 10). The ceramic surface was polished with 320-grit silicon carbide paper under running water, rinsed with water spray for 10 s and dried. The samples were then divided into two subgroups for mechanical surface preparation with hydrofluoric (HF) acid and Er YAG laser (2 W, 200 m J, 10 Hz, 10 s). Each group was divided into two subgroups for use/no use of silane. The conventional or universal adhesive was then applied on the samples iives.
The repair SBS was higher in groups treated with HF acid compared to laser. Ceramic surface treatment with HF plus silane plus conventional adhesive yielded a higher SBS as well as HF plus Universal adhesive. Thus, the application of silane as a separate step can be omitted in the repair of ceramic restorations with universal adhesives.
To compare and evaluate the strength rendering capacity of three restorative materials in tooth model simulated as immature teeth.
In this
study, 80 human maxillary permanent central incisors scheduled for periodontal extraction were collected, and an immature tooth model was prepared using a 3 mm twist drill. To simulate single-visit apical barrier, all the teeth were prepared with peso number 1-6. The teeth were segregated into three experimental and a control group. The experimental groups (
= 20) comprised of fiber-reinforced composite (FRC), Biodentine, and glass ionomer cement. The fracture resistance of all the teeth was tested using universal testing machine. The final reading of the applied load to cause fracture was noted and later was subjected to statistical analysis,
≤ 0.05 was considered statistically significant, and the level of significance was fixed at 5%. Student's
-test was applied to compare values among experimental groups.
There was a significant difference in the values of peak load resulting in fracture among experimental groups which was observed statistically (
≤ 0.001). FRC exhibited superior reinforcing capacity (mean 1199.7 N) among the experimental materials followed by Biodentine and Bioglass R. The lowest value to fracture was observed in control group (mean 236.7 N).
The results indicate that FRC could substantially contribute positively in reinforcing the simulated thin-walled immature roots.
The results indicate that FRC could substantially contribute positively in reinforcing the simulated thin-walled immature roots.The white spot lesion on the dental enamel is an optical alteration that compromises the esthetics of smile. It can be caused by many factors, among them, defects in mineralization and formation of tooth enamel. Resin infiltrants are agents that penetrate, by capillarity, through the pores of the demineralized or hypomineralized enamel, altering the refractive index (RI) of the tooth structure and totally or partially masking the appearance of the white spot. The aim of this work was to report the use of resin infiltration to minimize the visualization of white spot lesion, present in an upper central incisor, as a microinvasive approach for the esthetic treatment of deep enamel hypomineralization. A 20-year-old female patient sought care with the esthetic complaint of extensive white spot lesion on the buccal face of her upper right central incisor. The diagnosis established was a deep white stain associated with the incisor molar hypomineralization syndrome, and the treatment of choice for the resolution of the case was the application of the Icon® resin infiltrant (DMG, Hamburg, Germany). To reach the body of the lesion, three cycles of acid erosion, using 15% HCl, were necessary to obtain a satisfactory aspect of masking the white lesion. A camouflage effect of the deep white spot lesion was achieved with the use of the resin infiltration, without the need of additional and irreversible wear of the dental structure.
