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33 and .07 body weight, respectively. This model supports prior work in that intersegmental contact is a critical parameter when estimating tibial contact forces in high knee flexion movements across a range of culturally and occupationally relevant postures.The chemical analysis of archaeological ceramic artifacts can help archaeologists to characterize the technology used in the manufacturing process, and to elucidate aspects related to the way of life of past peoples. In this context, six ceramic fragments found at Tapera Beach in Florianópolis were analyzed. FTIR spectroscopy was used to identify carbonates and oxalic acid originating from the biodegradation process. In the pictorial regions of the ceramics, iron oxides, manganese and chromium were present, along with calcium carbonate, which were used as pigments. In addition, the absence of characteristic bands for kaolinite suggests low firing temperatures (up to 900 °C). EDS confirmed the elements of the molecules identified by FTIR and showed the presence of Al and Si in the primary structure and Fe in the secondary structure of the ceramics. In addition, there were traces of rare earth elements in samples A3 and A5, which may be related to the geochemistry at the site from which the raw material was excavated. The GC-MS results showed the presence of lipids and the profile of the fatty acids detected suggested that the source of the oils adsorbed in the ceramic structure is vegetable.The pandemic of coronavirus disease 2019 (COVID-19) showed a significant impact on routine daily services in departments of otorhinolaryngology head and neck surgery. The city of Wuhan, as the first reported epicenter in the world, resumed medical service since April 8, 2020. As the biggest ENT services provider in Wuhan, we share out institution's triage and screening system in the resuming period.

Current therapies for neuropathic pain are generally symptomatic and possess several side effects, limiting their prolonged usage.

Thus, it is urgent to develop novel and safe candidates for the management of this chronical condition. For this purpose, we investigated the analgesic effect of a standardized extract from Zingiber officinale Roscoe rhizomes (ZOE) obtained by CO

supercritical extraction, in a mice model of peripheral neuropathy. We also explored the mechanism of action of ZOE and its main constituents using an in vitro model of neuroinflammation.

Peripheral mono-neuropathy was induced in mice, by spared nerve injury (SNI). The analgesic effect of ZOE after oral administration was assessed by measuring mechanical and thermal allodynia in SNI mice. The mechanism of action of ZOE and its main constituents were investigated using spinal cords samples and in an in vitro model of neuroinflammation by ELISA, western blotting and immunofluorescence techniques.

Oral administration of ZOE 200 mg kg

ameliorated mechanical and thermal allodynia in SNI mice, with a rapid and a long-lasting effect. ZOE did not alter locomotor activity. In BV2 cells and spinal cord samples, ZOE, 6-gingerol and 6-shogaol reduced pERK levels, whereas ZOE and terpene fraction reduced HDAC1 protein levels, inhibited NF-κB signalling activation and decreased IL-1β, TNF-α and IL-6 release. ZOE and each tested constituent had a positive effect on inflammation-impaired SH-SY5Y cell viability.

The oral administration of ZOE attenuated SNI-induced neuropathic pain symptoms by reducing spinal neuroinflammation, suggesting ZOE as a novel and interesting candidate for the management of neuropathic pain.

The oral administration of ZOE attenuated SNI-induced neuropathic pain symptoms by reducing spinal neuroinflammation, suggesting ZOE as a novel and interesting candidate for the management of neuropathic pain.New, sensitive and rapid chiral HPLC method for enantioseparation and determination of widely used hypnotic drug, zopiclone, was developed. Enantioseparation was achieved on the immobilized amylose based Lux i-Amylose 1 column in polar organic mode in less than 7 min. The effects of the mobile phase composition, type and content of major components as well as acid/base ratio and column temperature on the retention and enantioseparation were investigated. The mobile phase consisted of acetonitril and methanol with small addition of triethylamine and acetic acid with a flow rate of 1 mL min-1. Calibration curves of both zopiclone enantiomers were linear over the concentration range of 5-125 μg mL-1. The limits of detection and quantification for (R)-zopiclone were 5 and 15 ng mL-1 and for (S)-zopiclone were 7 and 21 ng mL-1, respectively. It was demonstrated that the proposed method was selective, precise and robust. Finally, the validated method was applied to the determination of zopiclone enantiomers in the commercial tablets.Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal carcinomas due to an inefficient early diagnosis and a disastrous prognosis. AMD3100 High resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) detection of pancreatic tissues would facilitate the understanding of metabolomic characteristics of PDAC and further its clinical diagnosis by in vivo magnetic resonance spectroscopy (MRS). Pancreatic tissues from PDAC patients and Sprague-Dawley (SD) rats model and corresponding controls were detected and comparatively analyzed with HR-MAS NMR-based metabolomic strategy in order to get the underlying biomechanism and diagnostic information of PDAC. According to the univariate and multivariate statistical analysis, eight shared characteristic metabolites by PDAC patients and rats, including glycerophosphocholine (GPC), lactate, myo-inositol, methanol, taurine, methylene of lipid (L-CH2), β-glucose and phosphocholine (PC), were identified as potential biomarkers of PDAC. Especially, GPC, PC and myo-inositol demonstrated high levels in pancreatic tissue and kept consistent metabolic changes both in PDAC patients and rat models. The occurrence of PDAC mainly involved the aberrations in glycerophospholipid metabolism, galactose metabolism and taurine and hypotaurine metabolism. As an in vitro alternative technique of in vivo MRS, HR-MAS NMR provided good resolution and sensitivity, thus underpinning a potential translation to the in vivo MRS setting for noninvasive detection and monitoring of clinical PDAC. The metabolic differences caused in the different species not only enhance the understanding of metabolic reprogramming of PDAC but also promote the intercommunication of PDAC metabolomes between the different species.