Lauridsencrawford4332

Elucidating electrokinetic stability by which surface charges regulate toxins interaction with erythrocytes is crucial for understanding the cell functionality. Electrokinetic properties of human erythrocytes upon treatment of Vipoxin, phospholipase A2 (PLA2) and Vipoxin acidic component (VAC), isolated from Vipera ammodytes meridionalis venom were studied using particle microelectrophoresis. PLA2 and Vipoxin treatments alter the osmotic fragility of erythrocyte membranes. The increased stability of cells upon viper toxins is presented by the increased zeta potential of erythrocytes before sedimentation of cells during electric field applied preventing the aggregation of cells. Lipid peroxidation of low dose toxin-treated erythrocytes shows reduced LP products compared to untreated cells. The apparent proton efflux and conductivity assays are performed and the effectiveness PLA2 > Vipoxin>VAC is discussed. The reported results open perspectives to a further investigation of the electrokinetic properties of the membrane after viper toxins treatment to shed light on the molecular mechanisms driving the mechanisms of inflammation and neurodegenerative diseases.Polysaccharide foulant is known to play a crucial role in membrane fouling, however the detailed influential mechanisms and the pertinence to specific structure of polysaccharides, as well as intermolecular interactions among them with and without divalent cation are still indistinct. In this study, seven polysaccharides including agarose, sodium alginate, carrageenan, pectin, starch, sodium carboxymethylcellulose (CMC) and xanthan gum, with different chain and molecular structures, were used as model foulants to investigate the role of structural and functional features of polysaccharides in membrane fouling. Two Hermia's models (classical and mass-transfer models) as well as the resistance-in-series model were used to analyze the fouling mechanism. Results show that the spatial configuration of foulant molecule is significant in membrane fouling which actually controls the resistance of gel layer formed on membrane. Polysaccharides with different properties show distinct fouling mechanisms which are in accordance with the four models described by Hermia respectively. Cations may change the interaction of polysaccharide foulant which further leads to the structural change of the gel layer. It turns out that mass-transfer model is more suitable for interpreting of crossflow filtration data. So far, little has been known about the effects of molecule structure of polysaccharides on membrane fouling. In this paper, we provide a basic database for polysaccharide fouling which will work as a theoretical basis for finding more effective measures to prevent and control membrane fouling.Tropilaelaps mercedesae is not only a major threat to honey bees in Asia but also a potential risk to global apiculture due to trade. Imidacloprid is a systemic insecticide that negatively affects individual bees. Moreover, the health of honey bees may be threatened by imidacloprid exposure and T. mercedesae infestation. We studied the effects of T. mercedesae and imidacloprid on the survival, food consumption and midgut bacterial diversity of Apis mellifera in the laboratory. Illumina 16S rRNA gene sequencing was used to determine the bacterial composition in the honey bee midgut. T. mercedesae decreased survival in parasitized honey bees compared with nonparasitized honey bees, but there was no significant difference in food consumption. The imidacloprid 50 μg/L diet significantly decreased syrup consumption of A. mellifera compared with the control diet. The combination of T. mercedesae infestation and imidacloprid 50 μg/L exposure reduced survival and increased pollen consumption of A. mellifera. T. mercedesae infestation or a combination of T. mercedesae infestation and exposure to 25 μg/L imidacloprid affected the midgut bacterial composition of honey bees. T. mercedesae infestation and imidacloprid exposure may reduce the survival and affect honey bee health.Photoelectrocatalytic (PEC) water treatment is a promising technology for organic pollution abatement. Much of the prior research focused on material discovery and optimization. However, challenges exist in scaling-up PEC processes and are associated with designing reactors with effective light irradiation on electrode surfaces and, simultaneously, efficient electrode configurations. We design and demonstrate key reactor design principles, which influence reaction mechanisms, for a reactor using a TiO2 nanotube-coated disc flow reactor. Degradation of organochlorinated 2,4-dichlorophenol was studied as representative carcinogenic micropollutant. Mps1IN6 The synergistic photoelectrocatalytic process showed 5-fold faster degradation kinetics than solely electrocatalytic treatment or a greater than 2-fold enhancement over photocatalysis alone. Applicability of photoelectrocatalytic treatment was demonstrated over a wide range of micropollutant concentrations with almost complete abatement even at concentrations up to 25 mg L-1 of 2,4-dichlorophenol. Mechanistically, the increase in applied current density efficiency for degradation of 2,4-dichlorophenol was due to stabilization of charge carriers and higher oxidants production rates in the PEC system. Carboxylic acids were identified as the main by-products formed from cleavage of the phenolic ring moieties in 2,4-dichlorophenol. However, very importantly we achieved dehalogenation photoelectrocatalysis with evidence of chlorine heteroatoms released as innocuous chloride anions. Overall, this research demonstrates the importance of PEC reactor design and how properly orientated TiO2 nanotube-coated disc flow reactors leverage both novel material designs and reactor architectures to achieve pollutant degradation.We investigated the daily variations in the concentration of atmospheric dioxins (PCDD/Fs and DL-PCBs) in paddy fields after the autumn harvest. The geometric mean of the concentrations of dioxins during the research period was 0.042 pg-TEQ/m3, and the concentrations ranged from 0.0058 to 0.53 pg-TEQ/m3. When the weather was calm with no rain or snow and the atmosphere was stable, relatively high concentrations of dioxins were observed. The characteristics of congeners and homologues produced through the combustion of PCDD/Fs were classified into four groups using principal component analysis and cluster analysis. In addition, agricultural chemicals (pentachlorophenol, PCP and chlornitrofen, CNP) were identified as the source of PCDD/Fs using a Bayesian semifactor model. When the dioxin concentrations were high, the combustion of TeCDFs and PeCDFs produced large emissions, representing the impact of open burning of rice straw. When the dioxin concentrations were low, the contribution of CNP was strong, indicating the effects of the release of previously used agricultural chemicals from the soil.