Langleysiegel2223

The intracellular Qu content produced by dissociation of Qu-SS-Gcc LNPs was higher than that produced by internalization of free Qu. The resulting release of Qu exerted superior cell-killing effects on MCF-7/ADR cells, such as P-gp inhibition by binding to P-gp binding sites, blocking the cell cycle in the G2 phase, and causing cell apoptosis and autophagy. Moreover, it was revealed autophagy triggered by a low concentration of Qu-SS-Gcc LNPs was beneficial to cell survival, while at a higher concentration, it acted as a cell killer. Qu-SS-Gcc LNPs can realize massive accumulation of Qu in tumor cells and exert a multifaceted killing effect on tumor cells, which is a reference for the delivery of hydrophobic drugs.Since its invention in 1986, the polymerase chain reaction (PCR), has become a well-established method for the detection and amplification of deoxyribonucleic acid (DNA) with a specific sequence. Incorporating fluorescent probes, known as TaqMan probes, or DNA intercalating dyes, such as SYBR Green, into the PCR mixture allows real-time monitoring of the reaction progress and extraction of quantitative information. Previously reported real-time PCR product detection using intercalating dyes required melting curve analysis (MCA) to be performed following thermal cycling. Here, we propose a technique to perform dynamic MCA during each thermal cycle, based on a continuous fluorescence monitoring method, providing qualitative and quantitative sample information. We applied the proposed method in multiplexing detection of hepatitis B virus DNA and complementary DNA of human immunodeficiency virus as well as glyceraldehyde 3-phosphate dehydrogenase in different concentration ratios. We extracted the DNA melting curve and its derivative from each PCR cycle during the transition from the elongation to the denaturation temperature with a set heating rate of 0.8 K·s-1and then used the data to construct individual PCR amplification curves for each gene to determine the initial concentration of DNA in the sample. Our proposed method allows researchers to look inside the PCR in each thermal cycle, determining the PCR product specificity in real time instead of waiting until the end of the PCR. Additionally, the slow transition rate from elongation to denaturation provides a dynamic multiplexing assay, allowing the detection of at least three genes in real time.One-step total hydrogenation of furfural (FAL) toward tetrahydrofurfuryl alcohol in continuous flow using cheap transition metals still remains a great challenge. We herein reported the total hydrogenation of FAL over Ni (∼5 nm) nanoparticles loaded on TiO2-SiO2 composites with long-term stability. The TiO2-SiO2 composites comprise amorphous TiO x which was grafted on the silica aerogel by acetyl acetone-aided controlled hydrolysis of tetrabutyl titanate. The catalysts were characterized by several techniques including Brunauer-Emmett-Teller, X-ray diffraction, transmission electron microscopy, H2-temperature-programmed reduction, and H2-temperature-programmed desorption. The hydrogenation performances were systematically explored in terms of TiO2 content, Ni loading, liquid hour space velocity, and so forth. Ni nanoparticles in contact with amorphous TiO x showed strengthened interaction with the C=O bond of FAL as well as enhanced hydrogen dissociation and desorption ability, hence benefiting the overall hydrogenation process.In view of the current high cost of graphene, the corn flour with rich sources was selected as the raw material to prepare nano-graphene by the hydrazine hydrate (Hummers) redox method. The elements, structure, and morphology of the obtained corn graphene (CG) were studied by the organic element analysis, X-ray photoelectron spectroscopy, X-ray diffraction, Raman spectroscopy, atomic force microscopy, and transmission electron microscopy. It was found that the carbon content of CG was increased by 37.8% from 57.4% (corn flour) to 95.2% (CG). There was a diffraction peak of graphene on the (002) crystal surface at 23.08°. The D and G peaks of the Raman test were present, and the ID/IG of the peak intensity ratio was 1.19. The lattice distance of the CG sample was larger than that of the commercial graphene (GE), the CG was about three layers with a layer spacing of 1.21 nm, and the CG was thinner than the GE, which proved that the obtained CG was the nano-graphene.In this study, umbelliferone and α-cyclodextrin host molecules have been mixed up through a coprecipitation method to prepare a supramolecular complex to provide physical insights into the formation and stability of the inclusion complex (IC). click here The prepared hybrid was characterized by 1H nuclear magnetic resonance (1H NMR), Fourier transform infrared (FTIR) spectroscopy, electrospray ionization (ESI) mass spectrometry, DSC, and fluorescence spectroscopic studies. Job's plot provides a stoichiometric ratio of 11 and the Benesi-Hildebrand double reciprocal plot gives binding constant values using fluorescence spectroscopic titrations and the ESI mass data support the experimental observations. The results of molecular modeling were systematically analyzed to validate the inclusion complexation. In preliminary computational screening, α-cyclodextrin IC of umbelliferone was found to be quite stable based on the docking score, binding free energies, and dynamic simulations. In addition, the results obtained from 1H NMR and FTIR spectroscopy studies supported the inclusion complexation phenomenon. The results obtained from computational studies were found to be consistent with the experimental data to ascertain the encapsulation of umbelliferone into α-cyclodextrin.Monodisperse mesoporous silica nanoparticles (MMSNs) with fractal structures were synthesized via a facile, one-pot, surfactant-free process under the well-known Stüber synthesis condition. It was characterized by scanning electron microscope, transmission electron microscopy, and N2 adsorption-desorption isotherms. Phytase was immobilized on the MMSNs by physical adsorption. The enzyme loading capacity, activity, and release profile were measured by a faster and more reliable assay method, which was based on the hydrolysis of para-nitrophenylphosphate. The results show that the fractal structures have an important influence on the phytase capacity, and the releasing results also illustrated that phytase immobilized on MMSNs possessed the smallest releasing amounts under acidic conditions (pH = 3).