Lambertsenbrewer6941

induction of luteolysis to estrus alerts, estrus alert duration, and the onset of estrus alerts to ovulation interval were 72.2 ± 18.1, 13.5 ± 3.8, and 23.8 ± 7.1 h, respectively. We concluded that an ear-attached AED system that monitored physical activity and rumination time was effective at detecting cows in estrus and generated few false positive alerts when accounting for ovulation, cow physiological limitations, and the limitations of the RTE.This narrative literature review summarizes findings regarding the associations of clinical and subclinical hypocalcemia with postpartum health, reproduction, and milk production. To better understand the effects of hypocalcemia, we reviewed clinical and subclinical presentations of the condition and the dynamics of blood Ca concentration in the early postpartum period. We summarize and discuss the associations between hypocalcemia and performance of dairy cows. Up to 50% of dairy cows suffer from at least one disease event in the transition period. The important roles of calcium in muscle contraction and immune function make it a key component of metabolism, inflammation, and defense against infection. Although the effect of clinical hypocalcemia (milk fever) on health and performance is clear, the definition of subclinical hypocalcemia (SCH) and its consequences for health and performance are still ambiguous. selleck Differences in study designs, sampling protocols, Ca concentration thresholds, and sample sizes that may be underpowered for health and reproduction outcomes lead to inconsistent conclusions on the effects of SCH. On current evidence, classification of SCH should be based on at least 2 measurements of blood calcium, using cutpoints supported with relevant data, which may vary depending on the outcome of interest. Arbitrary or poorly supported interpretative thresholds for blood Ca concentrations should be abandoned. Transient SCH appears to be associated with greater milk yield, whereas SCH that is present several days after calving is associated with lesser production and greater disease risk. However, when blood calcium is measured days after calving, primary effects of calcium metabolism may be confounded by feed intake, inflammation, or disease, which might either contribute to or be a consequence of hypocalcemia. Additional research is needed to refine sampling schemes to classify SCH, and to better inform the goals and means of prevention of SCH.Bisphenol A (BPA) as a chemical hazard may enter the milk chain during primary production at the farm and, successively, during milk processing at a dairy company. To identify the potential pathways that play a role in the occurrence of BPA, a monitoring model for risk assessment based on the identification of the hazards at each stage of milk processing was investigated. Milk samples were analyzed using liquid chromatography with fluorescence detection. Quantifiable levels were detected in samples obtained from the raw milk storage tank, pasteurized milk from the storage tank, and packaged milk. The highest BPA contamination levels were detected in raw milk from the storage tank (mean 0.265 µg/L). Despite the fact that dietary exposure levels were below the temporary daily intake, BPA may have adverse effects, particularly for vulnerable population groups. New monitoring programs involving each stage of milk processing should therefore be applied.Kazakh cheese is a traditional dairy product in Xinjiang, China. To study the function and potential probiotic characteristics of yeast in Kazakh cheese and its contribution to cheese fermentation, we screened the γ-aminobutyric acid (GABA)-producing yeasts Pichia kudriavzevii 1-21, Kluyveromyces marxianus B13-5, Saccharomyces cerevisiae DL6-20, and Kluyveromyces lactis DY1-10. We investigated the potential probiotic properties of these strains and their use in cheese fermentation (cheeses designated CSP, CSM, CSS, and CSI, respectively); a control with no added yeast was designated CS. The results showed that the 4 yeast strains all showed high self-polymerization (2- and 24-h autoaggregation capacity of >80 and 90%, respectively), hydrophobicity (40-92% variation, low hydrophobicity in xylene, but within the range of probiotics), and the ability to survive the gastrointestinal tract (survival rate >75% after simulation), indicating the probiotic ability of the strains in vitro. The GABA production capacity ions of ethyl acetate, butanoic acid, ethyl ester, 3-methyl-1-butanol-acetate, ethyl hexanoate, ethyl octanoate, acetic acid 2-phenylethyl ester, and ethyl lactate; concentrations of 3-methyl-butanoic acid, propanoic acid, acetic acid, and butanoic acid were low. The CSP cheese had stronger acid-producing ability. The order of fragrance production performance was CSS > CSI, CSM > CSP > CS. Research into the fermentation mechanisms of GABA-producing yeast in cheese will provide a theoretical basis for the quality control and industrial production of Kazakh cheese.Monitoring rumination time (RT) around the time of calving is an effective way of identifying cows at risk of disease in early lactation. However, this only allows for the identification of cows a few days before the onset of clinical signs; thus, effective preventive measures cannot be implemented. Recent research has suggested that biomarkers of immune and metabolic function measured at dry-off (DO) can predict higher disease risk in early lactation. Nevertheless, the extent to which RT around DO is associated with early-lactation disease risk remains unexplored. Thus, the objective of this study was to compare RT in the weeks before and after DO between cows that did and did not experience health disorders in early lactation. For this, we conducted an observational retrospective cohort study utilizing the records available from a large commercial dairy herd in which RT is recorded daily using an automated system. Daily RT from -7 to +14 d relative to DO from 2,258 DO cycles and their respective health recoesulting in decreased RT for several days. Furthermore, the association between RT around DO and some early-lactation diseases suggests that RT could be a useful tool to identify at-risk cows early enough to allow for preventive interventions. Further studies should investigate the diagnostic utility of incorporating RT data early in the dry period in the disease prediction algorithms of rumination sensors.Social housing of preweaned calves can benefit their welfare, but housing and cross sucking are potential barriers to adoption for farmers. For farms using outdoor hutches, an option is to pair adjacent hutches with a shared fence. Our objective was to investigate milk- or starter-feeding strategies to mitigate cross sucking in this system. Holstein heifers were housed in pairs (n = 32 pairs) and divided into 4 treatments (n = 8 pairs each) in a 2 × 2 factorial design milk in an open bucket with starter in only a bucket (OB-SB), milk in an open bucket and starter in both a bucket and a specialized teat bottle (Braden bottle; OB-BB), milk in a slow-flow teat bucket with starter in only a bucket (TB-SB), or milk in a slow-flow teat bucket and starter in both a bucket and a specialized teat bottle (TB-BB). When starter was first offered (d 6 ± 1 of life, mean ± SD), calf latency to approach was recorded, averaged within pairs, and compared between starter treatments using a linear mixed model with fixed effect ouring weaning, when cross sucking increased overall (OB-SB 3.9 ± 0.4 min; OB-BB 1.8 ± 0.4 min; TB-SB 0.9 ± 0.4 min; TB-BB 1.6 ± 0.4 min). Regardless of starter treatment, calves spent less time cross sucking when fed milk in teat buckets, which extended the milk meal relative to open buckets (teat bucket vs. open bucket preweaning = 7.0 ± 0.2 vs. 1.6 ± 0.2 min; weaning = 3.0 ± 0.1 vs. 0.6 ± 0.1 min). When calves are fed milk in open buckets, a novel option for reducing cross sucking is to provide starter through a specialized bottle. Nonetheless, providing milk in slow-flow teat buckets had the greatest effect on reducing cross sucking by directing suckling to the teat instead of another calf or pen objects.The objectives of this study were to evaluate the effect of the metaphylactic use of a semi-synthetic long-acting macrolide (tildipirosin) on the prevention of pneumonia and otitis in preweaning Holstein calves, as well as its effects on the microbiome of their upper respiratory tract (URT) and feces. Newborn healthy Holstein heifers, collectively housed, were randomly allocated to 1 of 2 treatment groups treatment (TRT; n = 932) or control (CTR; n = 927). Calves in the TRT group received a single subcutaneous injection of 4 mg/kg tildipirosin (Zuprevo, Merck Animal Health) at 7 ± 7 d of life. Calves in the CTR group received no drug injection. All enrolled calves were evaluated from 1 to 63 ± 3 d of life (weaning age) and monitored daily for any adverse health events during this period. Daily physical examination was performed to diagnose pneumonia and otitis, and body weight was measured weekly in all animals. From a randomly selected subset of 217 calves, blood samples for biochemical variables analysis anin the CTR group. Furthermore, metaphylaxis had no significant effects on the total bacterial load, genus, or phylum analysis of the fecal microbiome from the 2 subset groups. However, for the URT microbiota, we observed a significant decrease in total bacterial load for the TRT group compared to the CTR group 1 week after metaphylactic injection. Tildipirosin metaphylaxis decreased the mean relative abundance of the genera Mannheimia, Moraxella, and Pasteurella but significantly increased the mean relative abundance of Mycoplasma. Although tildipirosin had no positive effect on Mycoplasma, it reduced the mean relative abundance of important pathogenic bacteria in the URT and had positive effects for the control of otitis. The metaphylactic use of tildipirosin can be a suitable strategy for the control of otitis on farms with a high prevalence of this disease.Measurements of energy balance (EB) require the use of respiration chambers, which are quite expensive and laborious. The GreenFeed (GF) system (C-Lock Inc.) has been developed to offer a less expensive, user friendly alternative. In this study, we used the GF system to estimate the EB of cows in early lactation and compared it with EB predicted from energy requirements for dairy cows in the Finnish feeding standards. We also evaluated the association between milk fatty acids and the GF estimated EB. The cows were fed the same grass silage but supplemented with either cereal grain or fibrous by-product concentrate. Cows were followed from 1 to 18 wk of lactation, and measurements of energy metabolism variables were taken. Data were subjected to ANOVA using the mixed model procedure of SAS (SAS Institute Inc.). The repeatability estimates of the gaseous exchanges from the GF were moderate to high, presenting an opportunity to use it for indirect calorimetry in EB estimates. Energy metabolism variables were not different between cows fed different concentrates. However, cows fed the grain concentrate produced more methane (24.0 MJ/d or 62.9 kJ/MJ of gross energy) from increased digestibility than cows fed the by-product concentrate (21.3 MJ/d or 56.5 kJ/MJ of gross energy). Nitrogen metabolism was also not different between the diets. Milk long-chain fatty acids displayed an inverse time course with EB and de novo fatty acids. There was good concordance (0.85) between EB predicted using energy requirements derived from the Finnish feed table and EB estimated by the GF system. In conclusion, the GF can accurately estimate EB in early-lactating dairy cows. However, more data are needed to further validate the system for a wide range of dietary conditions.