Kudskegelund1931

A novel biorefinery platform integrating thermal pretreatment and solid-liquid separation unit is here proposed to fully exploit food waste (FW) potential for production of valuable chemicals and energy through semi-continuous anaerobic bioconversion. The liquid fraction deriving from raw or pretreated FW, was fermented into volatile fatty acids (VFAs, from acetic to caproic acid) while the residual fraction was converted into biomethane. Thermal pretreatment effectively extracted a portion of the macromolecular organics, especially starch, to the liquid phase, promoting acidogenic fermentation and chain elongation pathways (0.43 gVFA g-1VSfed and 0.58 gVFA g-1VSfed with raw and pretreated extract, respectively). In parallel, anaerobic digestion of solid residue in 10 L reactors showed process stability and higher conversion rate for the pretreated residue (0.31 against 0.26 Nm3CH4 kg-1VSfed). The mass-transfer balance coupled with the economic assessment, calculated in terms of direct gross added value, indicated promising revenues by integrating the thermal upstream treatment.This study aimed to detoxify and degrade the organometallic pollutants from distillery wastewater by using an autochthonous microbial community via biostimulation and bioaugmentation process. Results revealed that the wastewater contained high concentrations of the metals i.e. Fe-2403; Zn-210.15; Cr- 22.825; Cu-73.62; Mg-27.30; Ni-14.425; and Pb-17.33 (mg L-1). The biostimulation and bioaugmentation process resulted from a substantial reduction (50-70%) in the pollution load. Scanning electron microscopy analysis showed bacterial community and their relationship with complex organometallic pollutants during the chemical reactions. The major identified organic pollutants in the control (untreated) samples were acetic acid, Oxo-,trimethylsilyl ester [CAS], Hydrocinnamic acid, p-[Trimethylsiloxy]-trimethylsilyl ester and tetradecanoic acid, trimethylsilyl ester [CAS] while some new metabolic products were generated as a by-product in bioaugmentation process. Therefore, the study showed that biostimulation and bioaugmentation were successful bioremediation strategies for the detoxification of distillery wastewater and restoration of organometallic polluted sites.The acquisition during biomass saccharification of elevated levels of fermentable sugars with lower cellulase concentration is central to ensuring an economically viable and industrially relevant hydrolytic process. Thus, using a new cellulase preparation (LT4) at low cellulase loading (2 mg protein/g dried substrate), this study assessed the possible boosting effect of integrating accessory enzymes and additives on high-solids hydrolysis of sugarcane bagasse via fed-batch feeding. Hydrolysis which commenced with initial 8% solids loading and subsequent substrate feeding of 4% solids at 6 h, 18 h, and 24 h respectively, proved optimal for the 20% high-solids saccharification producing 158 g/L total sugars and 83% glucose yield after 72 h with the combined optimized additives and accessory enzymes. The results obtained indicate that the integration of accessory enzymes and additives offers a benignant approach to minimizing the enzyme load and cost of high solids saccharification of lignocellulosic heteropolymers while also boosting enzyme hydrolytic performance.The integration of anaerobic digestion (AD) and pyrolysis (Py) could be a solution to economically utilize the organic fraction of municipal solid waste (OFMSW). However, it is not clear whether the environmental impact of the integrated pathway always outperforms the two single technologies. In this study, two integrated pathways (AD-Py, Py-AD) were compared with single AD and Py from the life cycle environmental impacts point of view. The results indicate that the environmental impacts of the four pathways are heavily dependent on their energy inputs and outputs. AD-Py is more environmentally friendly (-11.53 of total environmental impact /kg OFMSW) than single AD or Py. Py-AD exhibites the heaviest environmental burden (2.75 of total environmental impact /kg OFMSW) in all pathways. Therefore, AD-Py can be the top priority of treating OFMSW among the four pathways from the environmental viewpoint. This work could provide a theoretical support for the utilization of OFMSW.Carbon materials are promising in improving the performance of anaerobic digestion, however, interactive mechanisms between the carbon-based enhancement and operating parameters remained unclear. Using anaerobic digested sludge as inoculum, the effects of Taihu blue algae biochar (ABC) on methanogenesis at different inoculation ratios were investigated during sludge anaerobic digestion. Results showed that ABC enhanced methane productions at the lower inoculation ratios (4% and 1%, v/v), but not at the higher ratio (10%, v/v). Mechanism analysis demonstrated methanogenic improvements at the lower inoculation ratios were not owing to initial organic loading rate increments. Otherwise, ABC addition at the lower inoculation ratios were more favorable for the enrichment of Methanosarcina than the higher ratio, which might be benefit for methanogenesis through directed interspecies electron transfer. Thus, for the improvement of sludge anaerobic digestion, the microbial enrichments at different inoculation ratios would be more important than the merely biochar addition.

Perceived legitimacy associated with rules and authorities is an important element for understanding and encouraging compliance with rules in the field of road safety, often more so than with a deterrence approach. Despite a growing interest in legitimacy in recent decades and in the psychological field in particular, its definitions and measurements appear to be heterogeneous, subject to debate and in need of a common theoretical framework. Therefore, one can expect these limitations to also concern the definitions and measurements of legitimacy in the field of road safety. MEK inhibition However, no literature review related to this issue is currently available. This contribution therefore aims to fill this void by proposing a scoping review investigating, in the field of road safety, how legitimacy is defined, measured, related to compliance and what are its main determinants.

A scoping review was conducted, based on research literature from eight databases and concerned with the perceived legitimacy of traffic rulesinvestigating the outcomes of perceived legitimacy beyond compliance was identified. Few empirical studies have investigated the determinants of perceived legitimacy. Most identified empirical studies were cross-sectional, which is a limitation to establishing causality in investigated relationships. Theoretical implications and research perspectives to improve studies on legitimacy in the field of road safety and develop a multidimensional model of the perceived legitimacy of traffic rules are therefore proposed and discussed.Protected or separated bike lanes or cycle tracks, are increasing in popularity in North America and Europe. However, despite their documented benefits, there are concerns about potential conflicts between bicycles and vehicles when they merge back together at an intersection. The concern is that following a period of separation, drivers are less likely to anticipate and scan for the presence of bicyclists. This research examines how transitions from fully separated to mixed-traffic environments and vice versa affect driver behavior. The goal is to assess whether certain segment-intersection treatment combinations can alert drivers of the presence of bicyclists and thus, encourage them to scan for bicyclists prior to a right turn, reducing potential right-hook conflicts. Driving simulation is utilized and driver performance for right-turning vehicles is recorded under the presence of various bicycle infrastructure treatments along segments and at intersections. The experimental design includes conventional and protected bike lanes and intersections with either intersection crossing markings or protected intersections. The results show that the presence of protected bike lanes motivates fewer glances towards bicyclists traveling on those bike lanes, therefore, reducing drivers' ability to detect and in turn, perceive bicyclists. Drivers developed slightly lower speeds while driving next to protected versus conventional bike lanes. It was also found that protected intersections result in a higher rate of right glances at the intersection prior to a right turn. Drivers glancing at the intersection were also found to have lower speeds, indicating a correlation between the presence of protected intersection elements and speed selection. This research can be used to guide decisions on bicycle infrastructure implementation for safer multimodal operations.Data-based research approaches to generate crash scenarios have mainly relied on conventional vehicle crashes and naturalistic driving data, and have not considered differences between the autonomous vehicle (AV) and conventional vehicle crashes. As the AV's presence on roadways continues to grow, its crash scenarios take on new importance for traffic safety. This study therefore obtained crash patterns using the United States Department of Transportation pre-crash scenario typology, and used statistical analysis to determine the differences between AV and conventional vehicle pre-crash scenarios. Analysis of 122 AV crashes and 2084 conventional vehicle crashes revealed 15 types of scenario for AVs and 26 for conventional vehicles. The two groups showed differences in type of scenario, and differed in the proportion of crashes when the scenario was the same. The most frequent AV pre-crash scenarios were rear-end collisions (52.46%) and lane change collisions (18.85%), with the proportion of AVs rear-ended by conventional vehicles occurring with a frequency 1.6 times that of conventional vehicles. An in-depth crash investigation was conducted of the characteristics and causes of four AV pre-crash scenarios, summarized from the perspectives of perception and path planning. The perception-reaction time (PRT) difference between AVs and human drivers, AV's inaccurate identification of the intention of other vehicles to change lanes, and AV's insufficient path planning combining time and space dimensions were found to be important causes for the AV crashes. By increasing understanding of the complex characteristics of AV pre-crash scenarios, this analysis will encourage cooperation with vehicle manufacturers and AV technology companies for further study of crash causation toward the goals of improved test scenario construction and optimization of the AV's automated driving system (ADS).The loss of DYNLL1 contributes to chemoresistance in ovarian cancer. DYNLL1 binds to MRE11, a component of MRN complex (MRE11-RAD50-NBS1), and limits its function in homologous recombination (HR) repair in BRCA1-mutant cells. Decreased activity of MRE11 results in less HR-repair events and thus leads to higher sensitivity against DNA-damaging agents such as cisplatin. Therefore, a better understanding of the cellular changes in DYNLL1-MRN axis in ovarian cancer is needed. Here, we showed that DYNLL1 overexpression leads to decreased chemoresistance even in BRCA-proficient ovarian cancer cells. ATMIN, a transcriptional activator of DYNLL1, showed decreased expression; however, two components of MRN complex, MRE11 and NBS1 (NBN), showed increased expression in high grade compared to low grade serous ovarian cancer. We found that the components of MRN complex (MRE11-RAD50-NBS1) have higher protein levels in sites of omental metastasis and serous tubal intraepithelial carcinoma (STIC) compared to surrounding non-malignant stromal cells in patients with high grade serous ovarian cancer.