Krygerluna5214

22 J/cm2 , irradiation time=50 s). The bonding agent and PEEK opaque were applied on the surface of samples and they were veneered with a composite resin using a hollow plastic cylinder with an internal diameter of 4 mm. The SBS was then measured and the data were analyzed using one-way ANOVA, Tukey HSD test and Dunnett's test at 0.05 level of significance. Results The SBS of the 3 groups was significantly different (P less then 0.001). The Tukey HSD test revealed that the ErYAG laser had higher SBS than the CO2 laser group (P less then 0.001). The Dunnett's test showed that both ErYAG and CO2 laser groups yielded higher SBS than the control group (P less then 0.001). Conclusion The ErYAG and CO2 laser treatments can increase the SBS of PEEK to composite resin veneers, although the ErYAG laser seems to be more effective for this purpose. Copyright © 2020 J Lasers Med Sci.Introduction Remineralizing agents may be used for the treatment of white spot lesions (WSLs) prior to bracket bonding. However, some concerns exist regarding their possible interference with the etching and bonding process, negatively affecting the bond strength. This study aimed to assess the effect of two remineralizing agents with/without CO2 laser irradiation on the mechanical properties and shear bond strength (SBS) of demineralized enamel to the orthodontic bracket. Methods This study evaluated 60 premolar teeth in 6 groups (n=10) as follows (I) sound enamel, (II) demineralized enamel, (III) Nupro remineralizing agent (N), (IV) Nupro and CO2 laser (N/L), (V) Teethmate remineralizing agent (T), and (VI) Teethmate and CO2 laser (T/L). The remineralizing agents were applied to the enamel surfaces after their immersion in a demineralizing solution for 5 days. In groups IV and VI, the CO2 laser with a 10.6 μm wavelength, 10 ms pulse duration, a 50 Hz repetition rate, 0.3 mm beam diameter and 0.7 W power wasgents. The Ca/P ratio was the highest in the Nupro group and the lowest in the demineralized group. Conclusion Remineralizing agents can significantly improve the microhardness and structural properties of demineralized enamel to a level similar to that of sound enamel with no adverse effect on SBS to orthodontic brackets. Copyright © 2020 J Lasers Med Sci.Introduction In recent years, laser irradiation in the near-infrared ray (NIR) area has been reported to promote bone healing. There are also reports that laser irradiation accelerates orthodontic tooth movement. In this study, we investigated the effect of NIR laser irradiation and mechanical stimulation on osteoblasts. selleck chemicals Methods We seeded osteoblast-like cells and laser irradiation was performed 24 hours after cell seeding. In addition, a control group not receiving anything, a group receiving only Nd YAG (neodymium-doped yttrium aluminum garnet) laser irradiation, a group receiving only centrifugal loading, and a group receiving both Nd YAG laser irradiation and centrifugal force loading were set, and after 24 hours and after 48 hours, cells were collected and quantitative real-time polymerase chain reaction (PCR) was performed. Results 24 hours after laser irradiation, the gene expression of alkaline phosphatase (ALP), the receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) was significantly higher in the 2.0 W group than in the control group. In addition, the RANKL/OPG ratio was higher in the 2.0 W group than in the control group. Also, in the group using laser irradiation and centrifugal loading in combination, 24 hours after laser irradiation, ALP and OPG showed significantly higher values than those in the centrifugal load only group. Furthermore, the RANKL/OPG ratio also showed high values. Conclusion These results suggest that osteoblast-like cells activate genes related to bone metabolism by combining mechanical stimulation and laser irradiation. This helps to elucidate the influence of laser irradiation during tooth movement. Copyright © 2020 J Lasers Med Sci.Introduction As hair removal has become most popular in aesthetics, the management of its side effects is crucial for every practitioner. Available studies describe the effectiveness of the diode laser hair removal for all skin types according to the Fitzpatrick scale independently, but the question of the occurrence of side effects and adverse effects remains unanswered. This study aims to illustrate aspects of side effects for patients of various ethnicities and the impact of those on the effectiveness of the treatment. Methods The research was carried out in Poland and the United Kingdom from March 2016 to March 2019. 217 people of various ethnic origins were qualified for the study, 206 sessions were completed and statistical analysis was performed. The procedure was performed in the pubic area with diode lasers with a wavelength of 805 nm, minimum peak power of 2100 W and pulse duration between 15 and 400 ms. ET sapphire cooling assisted handle 9 x 9 mm large and pulse energy density between 10 and 100 J cases. The occurrence of side effects such as sensitivity and hyperpigmentation did not depend on the number of treatments. link2 However, the number of treatments had a significant impact on the incidence of erythema (P less then 0.001) and burns (P=0.005). More than half of the respondents (58.33%) had erythema after more than six procedures, whereas in the 6 treatments the erythema occurred only in 6.7% of cases. Conclusion The treatment with the use of diode lasers for all subjects with different ethnicity and thus the recognized scale according to Fitzpatrick is effective and safe. The occurring side effects are transient and do not impact the effect of the whole therapy. Copyright © 2020 J Lasers Med Sci.Introduction Dentin hypersensitivity is a common oral problem that occurs as a short and sharp pain. There are many techniques to treat this condition, the latest of which is laser treatment. The aim of this study was to evaluate the effect of two types of low-power diode lasers (660 nm and 810 nm) on dentin hypersensitivity in order to achieve an acceptable clinical application by adjusting the effective parameters. Methods In this randomized, double-blind clinical trial, sensitive teeth of 7 patients were divided into three groups with a randomized matching method group I, treated with 660-nm diode laser irradiation, group II, treated with diode laser 810-nm, and group III, the control group. Irradiation parameters for 660-nm and 810-nm diode lasers were the power of 30 mW and 100 mW respectively, in contact and continuous modes, perpendicular to the tooth surface with a sweeping motion. Treatments were carried out in four sessions at weekly intervals. The data obtained were analyzed with SPSS 22, using oner on reducing the dentin hypersensitivity was more long-lasting than that of the 660-nm laser. Copyright © 2020 J Lasers Med Sci.Introduction Low back pain (LBP) is a very common musculoskeletal disorder. The big burden of disease necessitates investigating a more effective modality of treatments with more persistence and also fewer side effects. Low power laser has been proved as a pain reducing modality, but there is a lack of studies comparing it with other treatments and also among the Iranian race and society. The purpose of this study was to evaluate the effect of low-level laser on patients with LBP. Methods Our study was a single-blind, randomized controlled trial. Forty subjects, aged between 20 to 70 with LBP participated in the study. Their pain severity scale was 3-10 according to the visual analogue scale of pain (VAS). link3 They were randomly assigned to two groups, a case group (true laser) and a control group (sham laser). Naproxen was prescribed with a free dose (250-1000 mg/ daily) to both groups. We evaluated patients' subjective pain, functional status (using the Roland Morris disability questionnaire), spinal range of mhs only in the case group (P less then 0.001). Regarding spinal tenderness, it was disappeared in 89.47% of the patients in the true laser group at the end of one month but remained unchanged in 73.33% of the subjects of the sham laser group. Conclusion We concluded that laser therapy (in combination with NSAIDs) is an effective and long-lasting therapeutic strategy in bringing relief from LBP without any significant side effect. Copyright © 2020 J Lasers Med Sci.Introduction The widespread application of lasers in medicine, especially in the treatment of diseases implies more investigations to understand the precious molecular mechanism of the laser effect on the human body. In the present study, the prominent role of HERC6 in response to CO2 Laser therapy of human skin is investigated. Methods The numbers of 16 gene expression profiles before and after the treatment with the CO2 laser are downloaded from Gene Expression Omnibus (GEO), and differentially-expressed genes (DEGs) are analyzed to find the significant DEGs. Gene ontology analysis revealed that HERC6 and a set of its neighbors played a significant role in response to laser application. Results The expression changes of 52 significant DEGs were compared via heat map analysis and 27 significant DEGs were introduced as the critical genes which are involved in response to laser irradiation. "Thymidylate kinase activity" among 9 clusters of biological terms was highlighted as an important biological process related to the identified DEGs. HERC2 was proposed as a critical DEG which was related to several essential cellular processes in response to laser application. Conclusion The findings from the present study indicate that HERC6 and the numbers of its first neighbors are involved in the essential cellular response to laser therapy of human skin. Copyright © 2020 J Lasers Med Sci.Nuclear factor- (erythroid-derived 2-) like 2 (Nrf2) is a regulator of many processes of life, and it plays an important role in antioxidant, anti-inflammatory, and antifibrotic responses and in cancer. This review is focused on the potential mechanism of Nrf2 in the occurrence and development of ocular diseases. Also, several Nrf2 inducers, including noncoding RNAs and exogenous compounds, which control the expression of Nrf2 through different pathways, are discussed in ocular disease models and ocular cells, protecting them from dysfunctional changes. Therefore, Nrf2 might be a potential target of protecting ocular cells from various stresses and preventing ocular diseases. Copyright © 2020 Ming-xuan Wang et al.Glutamic acid (Glu) is a worldwide flavor enhancer with various positive effects. However, Glu-induced neurotoxicity has been reported less. Tetrastigma hemsleyanum (TH), a rare herbal plant in China, possesses high medicinal value. More studies paid attention to tuber of TH whereas vine part (THV) attracts fewer focus. In this study, we extracted and purified flavones from THV (THVF), and UPLC-TOF/MS showed THVF was consisted of 3-caffeoylquinic acid, 5-caffeoylquinic acid, quercetin-3-O-rutinoside, and kaempferol-3-O-rutinoside. In vitro, Glu caused severe cytotoxicity, genotoxicity, mitochondrial dysfunction, and oxidative damage to rat phaeochromocytoma (PC12) cells. Conversely, THVF attenuated Glu-induced toxicity via MAPK pathways. In vivo, the neurotoxicity triggered by Glu restrained the athletic ability in Caenorhabditis elegans (C. elegans). The treatment of THVF reversed the situation induced by Glu. In a word, Glu could cause neurotoxicity and THVF owns potential neuroprotective effects both in vitro and in vivo via MAPK pathways.