Kromanndowns5060

Effective seizure control remains challenging for about 30% of epilepsy patients who are resistant to present-day pharmacotherapy. Novel approaches that not only reduce the severity and frequency of seizures, but also have limited side effects are therefore desirable. Accordingly, various neuromodulation approaches such as cortical electrical stimulation have been implemented to reduce seizure burden; however, the underlying mechanisms are not completely understood. Given that the initiation and spread of epileptic seizures critically depend on cortical excitability, understanding the neuromodulatory effects of cortical electrical stimulation on cortical excitability levels is paramount. Based on observations that synchronization in the electrocorticogram closely tracks brain excitability level, the effects of low-frequency (1 Hz) intracranial brain stimulation on the levels of cortical phase synchronization before, during, and after 1 Hz electrical stimulation were assessed in twelve patients. Analysis of phal excitability in the human brain. Low-frequency stimulation of the epileptic focus may therefore contribute to the prevention of impending epileptic seizures.Overexpression of the Bcl-2 protein has emerged as a hallmark of carcinoma cells and can be employed as a biochemical biomarker of these cells. Therefore, some Bcl-2 protein fluorescence probes (BPFPs) were designed for Bcl-2 protein quantification and carcinoma cells labeling. The high Bcl-2 protein binding affinity (Ki less then 1 nM) and selectivity (over 50,000-fold Bcl-2 protein selectivity against Mcl-1 protein) of BPFP1 endow it with the ability to detect trace amounts of Bcl-2 protein. After being incubated with a range of concentrations of Bcl-2 protein, BPFP1 exhibited the desired fluorescence properties and its fluorescence intensity is proportional to Bcl-2 protein concentration. Therefore, BPFP1 provides a convenient approach for Bcl-2 protein quantification and we could determine the concentration of Bcl-2 protein based on the BPFP1's fluorescence intensity. Subsequent studies revealed that BPFP1 can fluorescently label carcinoma cells by binding to overexpressed Bcl-2 protein in living cells, and can distinguish carcinoma cells (HL-60 cells and ACHN cells) from normal-tissue cells (HUVECs) according to the different Bcl-2 protein expression levels between carcinoma cells and normal tissue cells. In the present study, BPFP1 represents a new tool for Bcl-2 protein quantification, carcinoma cell visualization and cell sorting. Moreover, BPFP1 can be used in the future for early cancer diagnosis by detecting carcinoma cells in patient tissues.

This study aimed to examine the pregnant women's and their partner's experience of the information given at the antenatal clinic regarding screening methods.

A qualitative interview study was conducted in the Gothenburg area in Sweden. Ten women and seven partners expecting their first child were interviewed adjacent to the second-trimester ultrasound examination. The data were analysed using qualitative content analysis.

The findings present an overall theme, three main categories, and ten subcategories. The overall theme showed a nonhomogeneous and individually based picture concerning the meaning and purpose of the methods, need for improvement of information, parents own information seeking, and the encounter with the midwife was also experienced as an influential encounter. The main categories show that diversity relates to the level of information given, voluntariness, the possibility of abnormal findings, and varying needs to thorough information and pre-existing knowledge. The midwifés impact waions and discussion is as important as considering the parents' pre-existing knowledge, individual thoughts or questions, and a neutral attitude from the midwife is desirable.Slips, trips, and falls (STFs) are the second leading cause of non-fatal injuries and can lead to fatal incidents in the mining industry. Hazard identification is an essential first step in remediating STF hazards and creating a safer work environment. Previous research has identified industry-specific risk factors for STFs, evaluated exposures to those risk factors, and developed taxonomies of the hazards for the construction and farming sectors. In comparison, ErgoMine-a mobile device application-based ergonomics audit tool-is the only systematic evaluation tool that covers STF hazards in the mining industry. However, ErgoMine was not specifically developed to address STF hazards. This paper describes the development of a taxonomy that helps identify STF hazards at surface mining sites and provides recommendations to address these hazards to inform future evaluation tools. The objective was to develop a taxonomy that was self-explanatory, observable, repeatable, and solution oriented. In addition to current regulations, standards and guidelines were used to develop the taxonomy to ensure the focus was beyond basic compliance. A detailed description of how the STF hazard taxonomy was created for walkways, stairways, and fixed ladders is provided, along with two specific applications of its use. The STF hazard taxonomy can be used to develop tools like checklists and ergonomics audits to identify and remediate slip, trip, and fall hazards at surface mining facilities, thereby improving worker safety.Hazard perception assessment may benefit from VR-presentation by removing field-of-view restrictions imposed by single-screen tests. One concern is whether VR-induced 'cybersickness' will offset any benefits. Self-reported cybersickness ratings were recorded from 77 participants viewing two variants of a 360-degree hazard test hazard perception and hazard prediction. The latter was hypothesised to be particularly susceptible as clips abruptly cut to a probe question at hazard onset. Such sudden occlusions are thought to increase cybersickness. Overall cybersickness levels were low, with only four participants excluded for above-threshold sickness ratings. The remaining participants showed unexpectedly lower symptoms for the hazard prediction test and rated this test format as more comfortable and engaging. These findings mitigate concerns over the use of 360-degree videos in formative hazard assessments, even when clips involve sudden occlusions. Nonetheless, removal of any participants due to cybersickness raises problems for using VR for formal assessments of hazard perception skill.Globally, the walker is one of the most common assistive technologies used by older adults with mobility impairments, which is also the case in New Zealand. However, there is a scarcity of knowledge regarding their specific requirements in housing design. Adopting an ethnographic approach, the perceptions and spatial use of 16 older adults who used a walker were investigated as part of a larger study on quality of life and housing design. Five emergent themes for walker user perception and spatial use were identified, from which design considerations were distilled and then categorised into 10 design elements. The study highlights differences between the requirements for accommodating walkers and those for accommodating wheelchair users. It provides new insights into improved housing design for older adults, which have the potential to be incorporated into existing frameworks for accessible design and universal design thereby improving the independence and safety of older adults.Recently, zinc oxide nanoparticles (ZnONPs) are gaining much interest of nanobiotechnologists due to their profound biomedical applications. ZnONPs are used as antibacterial agents, which cause both gram-positive and negative bacterial cell death through the generation of reactive free radicals as well as membrane rupture. ZnONPs show excellent antioxidant properties in normal mammalian cells via the scavenging of reactive free radicals and up-regulation of antioxidant enzyme activities. Besides, it also shows hypoglycaemic effect in diabetic animals via pancreatic β-cells mediated increased insulin secretion and glucose uptake by liver, skeletal muscles and adipose tissues. Among the other potential applications, ZnONPs-induced bone and soft-tissue regeneration open a new horizon in the field of tissue engineering. Here, first we reviewed the complete synthesis routes of ZnONPs by physical, chemical, and biological pathways as well as outlined the advantages and disadvantages of the techniques. Further, we discussed the several important aspects of physicochemical analysis of ZnONPs. Additionally, we extensively reviewed the important biomedical applications of ZnONPs as antibacterial, antioxidant, and antidiabetic agents, and in the field of tissue engineering with special emphasis on their mechanisms of actions. Furthermore, the future perspectives of the ZnONPs are also discussed.Urban agriculture (UA) is a means for cities to become more resilient in terms of food sovereignty while shortening the distance between production and consumption. However, intensive soilless UA still depends on the use of fertilizers, which relies on depleting non-renewable resources such as phosphorous (P) and causes both local and global impact for its production and application. MF-438 With the aim to reduce such impacts and encourage a more efficient use of nutrients, this study assesses the feasibility of using struvite precipitated from an urban wastewater treatment plant as the unique source of P fertilizer. To do so, we apply various quantities of struvite (ranging from 1 to 20 g/plant) to the substrate of a hydroponic Phaseolus vulgaris crop and determine the yield, water flows and P balances. The results show that treatments with more than 5 g of struvite per plant produced a higher yield (maximum of 181.41 g/plant) than the control (134.6 g/plant) with mineral fertilizer (KPO4H2). On the other hand, P concentration in all plant organs was always lower when using struvite than when using chemical fertilizer. Finally, the fact that different amounts of struvite remained undissolved in all treatments denotes the importance to balance between a correct P supply to the plant and a decrease of P lost through the leachates, based on the amount of struvite and the irrigated water. The findings of this study show that it is feasible for UA to efficiently use locally recovered nutrients such as P to produce local food.Coagulation is the main process for removing natural organic matter (NOM), considered to be the major disinfection by-products (DBPs) precursor in drinking water production. In this work, k-means clusters analysis were used to classify influent waters from two different surface drinking water treatment plants (DWTPs) located in the Mediterranean region. From this, enhanced coagulation models based on response surface methodology (RSM) were then developed to optimise coagulation at two water catchments (river and reservoir). The cluster analysis classified the water quality of the raw waters into two groups related to baseline and peak organic loads. The developed enhanced coagulation models were based on the turbidity, total organic carbon (TOC) and UV254 removals. Sensitivity analysis applied to the models (after predictors selection) determined the factors relative individual contributions for each DWTP scenario. Then, profile plots for enhanced coagulation were studied to identify the optimal levels for each case.