Kramermendoza1949

Additionally, we also summarize the ongoing research focussed on the improved efficacy of chemotherapy and immunotherapy using the host microbiome.It was developed a material to act as an antimicrobial and antiparasitic agent through a modification reaction in the gum structure extracted from the plant Sterculia striata. This material was characterized, the oxidant activity was evaluated and the antimicrobial activity against Candida albicans, Escherichia coli, Pseudomonas aeruginosa, Salmonella Typhimurium and Klebsiella pneumoniae was investigated, in addition to the effect against Leishmania amazonensis, testing its acute toxicity and its cytotoxicity in human cells. Characterization techniques proved the success of chemical modification. The modification led to an increase in antioxidant activity, with excellent antibacterial activity, reaching almost 100% inhibition for P. aeruginosa and S. Typhimurium, and inhibitory effect above 70% against L. amazonensis, with an affinity far superior to the parasite than macrophages. The derivative showed no acute toxicity, it was non-hemolytic, increased cell viability in macrophages and fibroblasts, and stimulated cell proliferation of keratinocytes, thus being a strong candidate to be used as an antimicrobial and antiparasitic agent in biomedical applications.In this study, chitosan and gelatin materials incorporated with grape seed (Vitis vinifera L.) (VSE) and jabuticaba peel (Plinia cauliflora) (PPE) extracts were developed with potential application as food coatings. It was evaluated how the concentration of the extracts and their addition order in the polymeric matrix affect its properties. Samples with VSE presented a higher total phenolic content and also a more elastic behavior than samples with PPE. The addition order effect over viscosity was the opposite for the extracts, and for the samples with VSE a lower viscosity was obtained when the extract was added before gelatin. All samples were hydrophilic, a good result for application as coatings. Films with PPE were less soluble than chitosan/gelatin film, and CG5P sample was chosen as the most suitable for the desired application, due to its lower water vapor permeation value. The microbial permeation test showed that all samples avoid microorganism growth, extending shelf life of food. The results of this study revealed the extracts concentration was the main factor which influenced the studied parameters; however, their addition order had significant importance on rheological and barrier properties, the ones most influenced by the availability of extract compounds in the polymeric system.The reoccurrence of breast cancer is a major concern due to presence of cancer stem cells (CSCs). Considering the key role of hyaluronic acid (HA) in modulating the inflammation and cellular migration in cancer, the response of high molecular weight (HMW) and low molecular weight (LMW) HA towards various subtypes of breast cancer and breast cancer stem cells remain elusive. The aim of this study is to determine the effect of exogenous HMW-HA and LMW-HA on stemness of CSCs and epithelial-to-mesenchymal transition which may help in designing HA based therapeutic strategies. LMW-HA induces EMT in MCF-7 more prominently as compared to MDA-MB-231. However, HMW-HA did not show significant changes in the expression of EMT genes. Surprisingly, both HMW-HA and LMW-HA have shown to decrease the expression of EpCAM in MCF-7 cells and decrease the expression of CD44 in MDAMB-231 cells. HA has maintained the native stem cells phenotype of bCSCs isolated from MCF-7 only. The bCSCs isolated form MDAMB-231 showed a decrease in CD44. Luminal subtype has shown to follow Wnt/β-catenin whereas in the basal subtype localization of CD44 from surface to cytosol was observed in response to HA. NADPH-oxidase inhibitor Our study has demonstrated that bCSCs in luminal and basal cells follow differential intracellular signaling mechanisms in response to HA. This study could significantly influence the therapeutics involving HA in breast cancer.The multi-responsive controlled-release system could enhance crop yield while improving utilization efficiency of agrochemicals, and minimize environmental pollution caused by agrochemicals overuse. This work reports a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release. Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis. Moreover, the comprehensive performances, including photothermal conversion, water absorbency, water retention, and controlled-release agrochemicals behaviors, were systematically investigated. The results indicated that the composite microsphere was a prosperous water and agrochemicals manager to effectively retain water and control the release of agrochemicals by external stimulation. Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.The purpose of this study was to study polysaccharides isolated from Polygonatum sibiricum to establish the structure-activity relationships of the active substances and to discover the optimal fraction for further development and application. Four polysaccharides fractions (PSP1, PSP2, PSP3 and PSP4) from P. sibiricum were obtained by DEAE-Sepharose Fast Flow ion-exchange chromatography. Acid hydrolysis and FT-IR spectral and NMR spectral analyses were employed for structural analysis. Our results illustrated that PSP with different chemical structure and monosaccharide composition showed different abilities to activate phagocytic activity in vitro. According to the preliminary screening results in vitro, the newly identified water-soluble polysaccharides of PSP3 were selected for further evaluation in vivo. The results demonstrated that PSP3 possessed an immunomodulatory function and could be regarded as a promising candidate as an immunomodulator.(E)-2-(2-(2,3-dibromo-4,5-dimethoxybenzylidene)hydrazinyl)-4-(3,4-difluorophenyl) thiazole (DDH-1) is novel small molecule compound synthesized in our previous work. This study found that DDH-1 could inhibit the proliferation of various human lung cancer cells. Particularly, the IC50 for A549 cells was 8.59 μM. Interestingly, we found that DDH-1 inhibits eIF4E/eIF4G interaction. The flow cytometry (FACS) results have indicated that DDH-1 may induce G0/G1 cycle arrest by inhibiting the expression of Cyclin D1 and CDK4. DDH-1 may also cause apoptosis through the caspase-dependent pathway. Study of these mechanisms has shown that DDH-1 may prompt reactive oxygen species (ROS) generation, decrease the mitochondrial membrane potential (MMP), and stimulate DNA double-strand breaks (DSBs) in A549 cells. Study of the signal pathway has indicated that DDH-1 could activate JNK phosphorylation and inhibit ERK phosphorylation. Interestingly, NAC, which scavenges ROS, reversed the MMP decline, DNA damage, JNK phosphorylation activation, and ERK phosphorylation inhibition caused by DDH-1. link2 Overall, these findings provide evidence that the eIF4E/eIF4G interaction inhibitors DDH-1 induces DNA damage and apoptosis in human lung cancer A549 cells.Squid pen sample was treated by high hydrostatic pressure (HHP) prior to the extraction of chitosan. The physicochemical and antioxidant activities of the chitosan obtained with HHP (HHP-CS) were compared with chitosan of untreated squid pen sample (UT-CS). The chitosan extraction yield was optimized using response surface methodology, and the optimum condition was achieved at pressure of 500 MPa, extraction time of 10 min, and 1% (w/w) acetate concentration. The maximum yield of chitosan sample from the chitin of squid pens treated by HHP reached 81.9%. Among the process variables, the combined effects of pressure and acetate concentration significantly enhanced the extraction of chitosan from squid pens. The HHP-CS was found to be significantly effective in enhancing the fat binding capacity, water binding capacity, and water solubility index. SEM image analysis suggested that the HHP-CS had a rough surface with high porosity, while UT-CS exhibited a smooth surface. In vitro antioxidant assay suggested that HHP-CS had significantly higher DPPH radical scavenging activity, greater reducing power, and a stronger ferrous ion chelating effect than did UT-CS. Therefore, HHP can be an excellent alternative method for improving the physicochemical properties and antioxidant activities of chitosan from squid pens.We previously reported that chitinases reconstituted heat-inactivated stipe cell wall extension in a steady and continuous extension profile by cleaving chitins cross-linked to various polysaccahrides, whereas, endo-β-1,3-glucanases reconstituted heat-inactivated stipe wall extension in a profile of an initially fast extension and subsequent termination of extension due to its degradation of β-1,3-glucan but not other polysaccharides such as β-1,6-glucans cross-linked to chitins. link3 Thus, a novel endo-β-1,6-glucanase, GH30A, from Coprinopsis cinerea was cloned and characterized to study cross-linking of β-1,6-glucan and wall extensibility in stipe walls. GH30A had higher activity and better thermophilicity than reported β-1,6-glucanases. GH30A hydrolyzed pustulan having β-1,6-linkages but not other polysaccharides without β-1,6-linkages; GH30A did not cleave gentiobiose and single β-1,6-linkage branches in laminarin from Laminaria digitata but cut consecutive β-1,6-linkage branches in laminarin from Eisenia bicyclis. GH30A reconstituted heated-inactivated stipe cell wall extension with release of glucose and gentiobiose, indicating that β-1,6-glucans were present and cross-linked to chitins in stipe walls, and cleaving β-1,6-glucans cross-linked to chitins by GH30A led to wall loosening for extension. However, GH30A individually or in combination with endo-β-1,3-glucanase reconstituted-stipe wall extension profile was similar to individual endo-β-1,3-glucanase's, exploring that chitins were also cross-linked to other polysaccharides besides β-1,3-glucans and β-1,6-glucans.Recently, with the progression in tissue engineering, the importance of biocompatible nanocomposite film with suitable properties for potential applications in the biomedical area has been more developed. In this work, nanocomposite films of aldehyde-modified Carrageenan/Gelatin/halloysite nanotubes (AD-Carr/Gel/HNTs) nanocomposite films were successfully fabricated by the solution casting process. Halloysite nanotubes (HNTs) with different concentrations (0.5, 1.0, and 1.5 wt%) loaded into the aldehyde-modified Carrageenan/Gelatin (AD-Carr/Gel). Meanwhile, the developed AD-Carr/Gel/HNTs nanocomposite films were characterized by scanning electron microscopy (SEM), thermogravimetric analysis (TGA), mechanical property, water adsorption as well as in vitro degradation. The feasibility and capability of the nanocomposite films were further evaluated by hemocompatibility study, which showed that these nanocomposite films are hemocompatible. Besides, MTT assay revealed that the nanocomposite films are non-toxic, presenting the films as a good candidate to be used for tissue engineering purposes.