Koksummers6312

This study provides a proteome perspective on TMCC 70007, which was considered to be an important strain in the production of Pu-erh tea. The systematic proteogenomics analysis not only made a better annotation on the genome of B. adeninivorans TMCC 70007 as previous proteogenomics study but also provided solution for fermentation process protection on valuable industrial species with species specific genes uniquely identified from proteogenomics study.We review existing explanations for drop pinning and the origin of the force required to initiate the sliding of a drop on a solid surface (depinning). Theories that describe these phenomena include de Gennes', Marmur's, Furmidge's, the related Furmidge-Extrand's, and Tadmor's theory. These theories are all well cited but generally do not address each other, and usually papers that cite one of them ignore the others. Here, we discuss the advantages and disadvantages of these theories and their applicability to different experimental systems. Thus, we link different experimental systems to the theories that describe them best. We describe the force laws that can be deduced should these theories be united and the major open problems that remain. We describe a physical meaning that can be extracted from retention force measurements, specifically, the interfacial modulus that describes the tendency of a solid to conform to the liquid. This has implications for various wetting phenomena such as adhesion robustness, drug penetration into biological tissues, and solid robustness/resilience versus solid degradation over time as a result of its contact with a liquid.By combining small-angle X-ray scattering, wide-angle X-ray scattering, and rheology, the effect of additional polyelectrolyte chains on interactions among spherical polyelectrolyte brushes (SPB) was systematically investigated both on microscopic and macroscopic levels. The negatively charged poly(acrylic acid) (PAA) chains and positively charged poly(dimethyl diallyl ammonium chloride) (PDDA) chains were used as additional polyelectrolyte chains to investigate the local ordered structure and the "polyelectrolyte peak" among SPB. Interestingly, coacervation appeared in the SPB emulsion while introducing additional free polyelectrolyte chains. The addition of excess positively charged PDDA chains would lead to the transformation of the SPB emulsion from the coacervation to the aggregation, while it has not been observed in the case of PAA chains. Moreover, it was further confirmed that the specific local ordered structure was caused by the electrostatic interaction among polyelectrolyte chains of adjacent SPB. This work could enrich our understanding of polyelectrolyte assembly in concentrated SPB, thereby greatly broadening the application fields of SPB.Citrullination is an important post-translational modification implicated in many diseases including rheumatoid arthritis (RA), Alzheimer's disease, and cancer. Neutrophil and mast cells have different expression profiles for protein-arginine deiminases (PADs), and ionomycin-induced activation makes them an ideal cellular model to study proteins susceptible to citrullination. We performed high-resolution mass spectrometry and stringent data filtration to identify citrullination sites in neutrophil and mast cells treated with and without ionomycin. We identified a total of 833 validated citrullination sites on 395 proteins. Several of these citrullinated proteins are important components of pathways involved in innate immune responses. Using this benchmark primary sequence data set, we developed machine learning models to predict citrullination in neutrophil and mast cell proteins. We show that our models predict citrullination likelihood with 0.735 and 0.766 AUCs (area under the receiver operating characteristic curves), respectively, on independent validation sets. In summary, this study provides the largest number of validated citrullination sites in neutrophil and mast cell proteins. The use of our novel motif analysis approach to predict citrullination sites will facilitate the discovery of novel protein substrates of protein-arginine deiminases (PADs), which may be key to understanding immunopathologies of various diseases.When proteins in aqueous solutions are exposed to solid substrates, they adsorb due to the dynamic interplay of electrostatic, van der Waals, and hydration interactions and do so in a rather irreversible fashion, which makes protein recovery troublesome. Here, we use a gold electrode as the solid substrate and modulate the surface potential to systematically induce protein adsorption as well as partial desorption. We use different methods such as surface plasmon resonance, atomic force microscopy, and electrowetting and show that biasing the electrode to more negative potentials (by -0.4 V compared to the open-circuit potential at pH 6) results in an increased adsorption barrier of 6 kJ mol-1 for the negatively charged protein β-lactoglobulin. Further, we clearly demonstrate that this is due to an increased double layer potential of -0.06 V and an increase in hydration repulsion. This indicates that an electric potential can directly influence surface interactions and thus induce partial β-lactoglobulin desorption. These observations can be the basis for biosensors as well as separation technologies that use only one trigger to steer protein ad- and desorption, which is low in energy requirement and does not generate large waste streams, as is the case for standard protein separation technologies.For lateral flow immunoassay (LFIA), it is an important challenge to enhance the detection sensitivity to the same level as polymerase chain reaction or enzyme-linked immunosorbent assay to make LFIA pervasive in the field of on-site environmental analysis. We recently demonstrated that the LFIA sensitivity is dramatically enhanced by using Pt-nanoparticle-latex nanocomposite beads (Pt-P2VPs) as probes for the detection of the influenza A (H1N1) antigen compared with using conventional Au colloids as probes. RIP kinase inhibitor Here, to further enhance the LFIA sensitivity using Pt-P2VPs, superparamagnetic iron oxide nanoparticles (SPIONs) were chemically conjugated to Pt-P2VPs (Pt-P2VP@SPION) to give them magnetic separation capability (enrichment and/or purification). To investigate the effect of magnetic enrichment on the LFIA sensitivity in a sandwich format, the C-reactive protein (CRP) was chosen as a model analyte and anti-CRP antibody (CRPAb)-conjugated Pt-P2VP@SPION (Pt-P2VP@SPION-CRPAb) beads were used as probes. The visual limit of detection (LOD) of LFIA was successfully lowered by increasing the magnetic enrichment factor φ.