Knudsenbuchanan5953
Following from the relatively large nitrogen abatement targets, the overall effect of assuming catchment homogeneity is to underestimate the abatement costs and to overestimate the potential of wetlands to reduce nitrogen.Congenital erythrocytosis (CE) can be classified as primary and secondary and 82 consecutive patients of erythrocytosis who were JAK-2 mutation negative, were further investigated. The genomic DNA was extracted from all the patients and the EPO-R, VHL, EGLN1 and EPAS1 genes were PCR amplified and sequenced. The sequence analysis showed (28/82) 34.14% patients had mutations. Among them, (19/28) 67.86% patients had mutations in exon 8 of EPO-R gene, of which six were novel missense mutations, p.(Gly418Ala), p.(Gly390Ala), p.(Ala411Thr), p.(Gly475Val), p.(Glu490Asp), p.(Glu362Gln) and three were novel frameshift mutations, p.(Glu336*), p.(Pro327Hisfs*68), p.(Gly479Alafs*37). All these EPO-R patients were heterozygotes and were forming endogenous erythrocyte colonies (EEC). Some patients (8/28) 28.57% had mutations in VHL gene, out of which 3 novel homozygous missense mutations in exon 1 of VHL gene, p.Gly80Asp, p.Gln107Glu and p.Gln113Glu, were identified. In addition, (1/28) 3.5% patients had one reported heterozygous missense mutation in exon 12 of EPAS1 gene p.Gly537Arg and one novel frameshift mutation p.(Ala553Glyfs*58). Further, in silico analysis indicated most of the mutations, probably, were damaging the protein structures, causing the CE in these patients. In this study the mutations in EPO-R and EPAS1 genes were identified for the first time in India.Mindfulness meditation might improve a variety of cognitive processes, but the available evidence remains fragmented. This preregistered meta-analysis (PROSPERO-CRD42018100320) aimed to provide insight into this hypothesis by assessing the effects of brief mindful attention induction on cognition. Articles were retrieved from Pubmed, PsycInfo and Web of Science up until August 1, 2018. A total of 34 studies were included. The outcomes were categorized into four cognitive domains attentional functioning, memory, executive functioning and higher-order function. Selleck PHI-101 A small effect was found across all cognitive domains (Hedges' g = 0.18, 95% IC = 0.07-0.29). Separated analyses for each cognitive domain revealed an effect only in higher-order cognitive functions (k = 10, Hedges' g = 0.35, 95% IC = 0.20-0.50). Results suggest that mindfulness induction improves cognitive performance in tasks involving complex higher-order functions. There was no evidence of publication bias, but studies generally presented many methodological flaws.In this paper, theoretical study on molecular geometry, vibrational, pharmaceutical and electronic properties of the monomeric and dimeric structures of 1-benzothiophene-2-carboxylic acid (2BT) were carried out using B3LYP hybrid functional with 6-311++G(d,p) as basis set. The structural study show that the stability of 2BT crystalline structure arising from O-H…O, C-H…O as well as S-H…O hydrogen bonding interactions. Vibrational analysis, for monomer and dimer species, show a good compatibility between experimental and theoretical frequencies. Then, the 1H and 13C NMR chemical shifts were calculated using Gauge Independent Atomic Orbital (GIAO) technical. In addition, the UV-Vis spectrum was simulated in gas phase and in water throughout TD-DFT calculation. The electronic transitions were identified based on HOM-LUMO energies. However, donor-acceptor interactions and charge delocalization has been studied via natural bond orbital (NBO). The nucleophilic and electrophilic site localization is identified by molecular electrostatic potential. Hirshfeld surface analysis has been discussed based on color code demonstrating the various non covalent interactions. Besides, molecular docking analysis was reported to evince the pharmaceutical properties of the studied molecule.The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to the coronavirus disease 2019 (COVID-19) worldwide pandemic. This unprecedented situation has garnered worldwide attention. An effective strategy for controlling the COVID-19 pandemic is to develop highly accurate methods for the rapid identification and isolation of SARS-CoV-2 infected patients. Many companies and institutes are therefore striving to develop effective methods for the rapid detection of SARS-CoV-2 ribonucleic acid (RNA), antibodies, antigens, and the virus. In this review, we summarize the structure of the SARS-CoV-2 virus, its genome and gene expression characteristics, and the current progression of SARS-CoV-2 RNA, antibodies, antigens, and virus detection. Further, we discuss the reasons for the observed false-negative and false-positive RNA and antibody detection results in practical clinical applications. Finally, we provide a review of the biosensors which hold promising potential for point-of-care detection of COVID-19 patients. This review thereby provides general guidelines for both scientists in the biosensing research community and for those in the biosensor industry to develop a highly sensitive and accurate point-of-care COVID-19 detection system, which would be of enormous benefit for controlling the current COVID-19 pandemic.Particle-induced gamma-ray emission (PIGE), which measures prompt gamma rays at 428 keV from 10B (p, p'γ) 7Be, was used to confirm the boron distribution within 2hr-BPA-exposed cells. Distribution images of potassium, phosphate, and boron and the whole spectrum showed the ratios of boron counts to total (%) as follows control group 1.35 ± 0.073%; 2hr boron exposure group 2.33 ± 0.35%; and boron exposure/wash group 1.58 ± 0.095%. Micro-beam PIXE/PIGE can be a promising tool for visualization of intracellular Boron.The Nuclear Engineering Department of the Military Institute of Engineering (SE/7-IME) is designing and simulation a neutron irradiator with 1 Ci of 241Am-9Be source. The objective of this irradiator is to generate a flux of neutrons to be used in research and teaching maintaining, for purposes of radiological protection, the rate of ambient dose equivalent, H*(10), below 10 μSv/h at 30 cm from the surface. This paper presents the proposed irradiator, values of H*(10) at different distances from the irradiator and the neutron flux in different points of the beam irradiation, all calculated using the MCNPX code.This experimental visualization study was conducted to investigate and define the phenomena of an initially pressurized liquid water target that can prevent the boiling of water when the target is irradiated with a 30-MeV proton beam produced using the MC-50 Cyclotron at Korea Institute of Radiological and Medical Sciences. At various initial pressures and proton beam currents, the behavior of the target water was investigated using a complementary metal-oxide-semiconductor camera. We confirmed that an appropriate initial pressure could indeed prevent local bulk boiling, and be determined by solving Rayleigh's equation and the Clausius-Clapeyron equation for homogeneous bubble growth using the measured bubble size generated at the Bragg-peak region. The saturation temperature of the initial pressure must be higher than the calculated local water temperature at the Bragg-peak region. The final pressure of the water target increased proportionally with the initial pressure and proton beam current. The penetration depth of the beam varied with beam current and slightly with the final pressure, as evidenced by the emission of blue light in all experimental cases.XRF is a suitable tool for studies of cultural heritage, since it provides valuable information about the chemical composition of an artefact by means of a non-invasive, multi-elemental and non-destructive in situ analysis. This work presents and discuss the technical examination of the Portrait of a Young Man with a Golden Chain (c. 1635), a wood panel historically attributed to Rembrandt van Rijn and/or atelier, from the São Paulo Museum of Art (MASP) collection. Rembrandt was a Dutch painter, considered one of the greatest visual artists in the history of art. Besides his great success as a historical painter and portraitist, Rembrandt ran large workshops and had a large number of pupils and assistants. In situ PXRF were performed for the elemental characterization of the pigments presents in the painting. The results make possible the characterization of the original materials presents in the painting. Some of the materials identified were Lead White, Chalk, Vermilion, Bone Black, Azurite, Smalt, and earth pigments, such as Red, Yellow and Brown Ochres.
To verify if differential diagnostic algorithms based on selected parameters of dynamic and diffusion-weighted MRI, clinical and demographic variables are accurate enough to differentiate between parotid malignancies, pleomorphic adenomas and Warthin tumors.
The study included 84 patients, among them 19 (23%) with postoperative diagnosis of a parotid malignancy, 37 (44%) with Warthin tumors and 28 (33%) with pleomorphic adenomas. Accuracy of dynamic and diffusion-weighted MRI parameters (T
, T
, WR and ADC), clinical parameters of the tumor (location in the superficial or deep parotid lobe, single vs. multiple lesion, concomitant lymphadenopathy) and demographic characteristics of the patient (age, sex) was determined on ROC analysis.
Compared to Warthin tumors, parotid malignancies showed higher T
, T
and ADC, lower WR, were more often located in the deep lobe of the parotid and presented as single lesions with concomitant lymphadenopathy. Consideration of all these variables provided 100% sensitivity and 89.2% specificity of the differential diagnostic algorithm. Compared to pleomorphic adenomas, parotid malignancies presented with lower ADC and T
, higher T
, were more often located in the deep lobe of the parotid, associated with concomitant lymphadenopathy, diagnosed in men and individuals older than 66years. Consideration of all these variables provided 100% sensitivity and 96.4% specificity of the differential diagnostic algorithm.
Combined analysis of clinical data and results of dynamic and diffusion-weighted MRI may provide nearly ideal accuracy in differential diagnostics of parotid malignancies and the two most common histological types of benign tumors.
Combined analysis of clinical data and results of dynamic and diffusion-weighted MRI may provide nearly ideal accuracy in differential diagnostics of parotid malignancies and the two most common histological types of benign tumors.
To evaluate the impact of progressively increasing margin distances on locoregional recurrence-free survival (LFRS) and determine an optimum margin distance for tongue cancers.
We retrospectively reviewed the records of patients with squamous carcinoma of oral tongue, surgically treated between January 2012 and December 2013. The patients were divided based on ROC derived optimal distance and differences in LRFS per millimeter margin distances with no significant difference in LRFS starting from 0mm onwards (Group-I), from upper limit of Group-I to ROC cut-off (Group-II), and all values above it (Group-III). Group-II and III were matched for clinicopathologic factors and type of adjuvant therapy received.
451 patients had a median LRFS of 29.4months that included 32.1% of Stage I/II and 51.7% of Stage III/IV. Group-I consisted of 0--2.0mm (2.0-2.1mm (p-0.029)), Group-II form 2.1 to 7.5 (7.5-7.6mm (p-0.042)) and Group-III were≥7.6mm. In the matched group, each millimeter increase in margin provided a 3.67months survival advantage from 2.
