Klosteroliver5446

37 kg/s. With a deeper insight into the law of gas transfer and an elaborate consideration of the whole reservoir, this study is expected to provide a new approach and technical support to estimate the CO2 emissions accurately in reservoirs.Aquifers supply water to millions of farms, thousands of cities, and billions of people worldwide. Water use and economic activity in aquifer-dependent regions cannot be sustained if groundwater levels are not stabilized. This article addresses a question relevant to these regions internationally how can water scarce areas reduce aquifer depletion while supporting the many economically and institutionally important uses of groundwater, which serve as a critical source of supply in many parts of the world with limited or seasonal precipitation which could become more pronounced in the face of future climate stress. Facing that challenge, this work presents a framework for discovering measures to hydrologically stabilize aquifers that control economic losses while respecting local institutional constraints. It advances our capacity to discover measures to efficiently, equitably, and sustainably allocate burden sharing that protect aquifers while adapting to hydrologic, economic, and institutional characteristics of an affected community. Results of this work show that for the aquifers investigated, present practices of groundwater use are unsustainable and finds that alternative practices are possible. It provides scenarios describing such practices and also determines their hydrological and economic consequences. Finally, it shows how these results can feed into policy debates over the several water-sharing arrangements. This work makes several incremental contributions calibrating modelled pumping patterns to the historical baseline, controlling economic costs of achieving hydrologic sustainability, respecting institutional constraints governing equitable burden sharing, presenting an approach with powers of generalizability, and using routinely collected data. While the approach and findings are illustrated for two aquifers in Africa, its approach carries some generalizability. All data, variables, equations, constraints, and results are included as appendices.As a core component of the biomass, the important role of extracellular polymeric substances (EPS) on treatment performance has been recognized. However, the comprehensive understanding of its correlation with nitrogen removal remains limited in biofilm-based reactors. In this study, the relevance between EPS and advanced nitrogen removal in a novel step-feed three-stage integrated anoxic/oxic biofilter (SFTIAOB) was specifically investigated. The operation showed as high as 81% TN removal was achieved under optimal conditions. Among the whole reactor, 2nd anoxic (A2) zone was the largest contributor for nitrogen removal, followed by the 3rd anoxic (A3) and 2nd oxic (O2) zones. EPS composition analysis found that high content of polysaccharides in tightly bound-EPS (A2 and A3) and protein in loosely bound-EPS and tightly bound-EPS (O2). Fourier transform infrared spectroscopy, three-dimensional fluorescence spectrum further verified stratified EPS subfractions containing different secondary protein structures, while 3-turn helix and tryptophan-like protein was the main reason for nitrogen removal. High-throughput sequencing revealed the co-existence of nitrogen removal-associated genera accomplished nitrification/denitrification combined with aerobic denitrification and anammox. Moreover, the correlation of EPS and microbial composition with nitrogen removal was clarified by redundancy analysis (RDA). Finally, potential mechanism for nitrogen removal was illuminated. This research gives more insight into EPS characteristics in enhancing nitrogen removal during the operation and optimization of a step-feed multi-stage A/O biofilm process.Increasing anthropogenic pressures have affected the status of surface freshwater ecosystems. Eutrophication, water browning, acidification, and several other processes may be channelled through the food web. In this study, we evaluate the role of hydrology impacting anthropogenic pressures, flows from urban, farmland and ditched forest areas, and how they explain the physico-chemical quality of lakes and ponds in the boreal biome of Finland. We study the long-term effect around 445 waterfowl survey sites that had physico-chemical measurements (total phosphorus, total nitrogen, pH, water clarity and colour) produced by Finnish environmental authorities done in years 1986-2020. Furthermore, we investigate whether a long-term national-level citizen science study focusing on rather robust visible habitat structures measured by the volunteers can reveal physico-chemical water quality using data from >270 lakes where the waterfowl habitat survey and physico-chemical measurements could be spatio-temporally matched.nderstanding of biogeochemical processes in lake ecosystems and the primary sources of the nutrient and sediment loading.Dissolved organic matter (DOM) is an important organic matter fraction that plays a key role in many biological and chemical processes in soil. The effect of biochar addition on the content and composition of soil organic matter (SOM) and DOM in an agricultural soil in central Italy was investigated within a two-year period. UV-Vis spectroscopy and analytical pyrolysis have been applied to study complex components in DOM soil samples. selleck compound Additionally, analytical pyrolysis was used to provide qualitative information of SOM at molecular level and the properties of biochar before and one year after amendment. A method was developed to quantify biochar levels by thermogravimetric analysis that enabled to identify deviations from the amendment rate. The water-soluble organic carbon (WSOC) in the amended soils were significantly lower than those in the control soils, indicating that biochar decreased the leaching of DOM. DOM in treated soils was characterized by a higher aromatic character according to analytical pyrolysis and UV-Vis spectroscopy. Moreover, a relatively high abundance of compounds with N was observed in pyrolyzed of treated soils, suggesting that biochar increased the proportion of microbial DOM. The results from thermal and spectroscopy techniques are consistent in highlighting significant changes in DOM levels and composition due to biochar application with important effects on soil carbon storage and cycling.Recent studies have shown that the relationships between ecosystem services (ES) and human wellbeing (HWB) can be positive, negative, or non-existent, but the underlying causes and processes remain unclear. This study aimed to investigate how and why the local level ES-HWB relationship would change geospatially and manifest on broad regions. Using data for Mainland China, we first calculated seven ES and Human Development Index (an indicator of HWB), then used geographically weighted regression and cluster analysis to quantify the county-level ES-HWB relationship, and finally adopted Wilcoxon test and random forest to investigate key influencing factors. We found that (1) the local-scale relationship between ES and HWB exhibited a great deal of spatial heterogeneity, varying from positive to negative or no correlations across broad regions; (2) the varying relationships merged spatially into three general types of regions Positive Correlation-Dominant Region, Negative Correlation-Dominant Region, and No Correlation-Dominant Region; and (3) the variations and spatial patterns of the ES-HWB relationships were influenced by a number of social-ecological factors (e.g., population density and land cover compositions), and generally corresponded to different stages of land use transition and socioeconomic development a positive ES-HWB relationship was found mainly in socioeconomically underdeveloped (rural or agricultural) regions with low ES production levels; a negative ES-HWB relationship occurred mostly in intermediately developed regions with abundant non-food ES; and ES and HWB had no relationships in socioeconomically well-developed (intensive agriculture/urbanized) societies with ample provisioning ES. These findings suggest that neither the "environmentalist's paradox" nor the "environmentalist's expectation" adequately accounts for the complexity of the ES-HWB relationship.Wastewater surveillance of SARS-CoV-2 has become a promising tool to estimate population-level changes in community infections and the prevalence of COVID-19 disease. Although many studies have reported the detection and quantification of SARS-CoV-2 in wastewater, remarkable variation remains in the methodology. In this study, we validated a molecular testing method by concentrating viruses from wastewater using ultrafiltration and detecting SARS-CoV-2 using one-step RT-qPCR assay. The following parameters were optimized including sample storage condition, wastewater pH, RNA extraction and RT-qPCR assay by quantification of SARS-CoV-2 or spiked human coronavirus strain 229E (hCoV-229E). Wastewater samples stored at 4 °C after collection showed significantly enhanced detection of SARS-CoV-2 with approximately 2-3 PCR-cycle threshold (Ct) values less when compared to samples stored at -20 °C. Pre-adjustment of the wastewater pH to 9.6 to aid virus desorption followed by pH readjustment to neutral after solid removal significantly increased the recovery of spiked hCoV-229E. Of the five commercially available RNA isolation kits evaluated, the MagMAX-96 viral RNA isolation kit showed the best recovery of hCoV-229E (50.1 ± 20.1%). Compared with two-step RT-qPCR, one-step RT-qPCR improved sensitivity for SARS-CoV-2 detection. Salmon DNA was included for monitoring PCR inhibition and pepper mild mottle virus (PMMoV), a fecal indicator indigenous to wastewater, was used to normalize SARS-CoV-2 levels in wastewater. Our method for molecular detection of SARS-CoV-2 in wastewater provides a useful tool for public health surveillance of COVID-19.Nitrous oxide (N2O) is considered a powerful greenhouse gas. Vegetated ditches are an important source of N2O emissions in the agricultural systems. However, few studies have examined on the relationship between N2O emissions and the water level in vegetated ditches. To investigate the effect of water level on the N2O emissions, three pilot-scale ditches vegetated with Myriophyllum aquaticum were constructed with low (LW), medium (MW), and high (HW) water levels. The examined results indicated that the M. aquaticum ditches decreased N2O emissions by 38.4% and 67.9% in MW and HW, respectively, as compared to the LW ditch. In addition, the N2O emission factor decreased with increasing water level in the order of LW (0.18%) > MW (0.11%) > HW (0.06%). The MW and HW ditches reduced the N2O emissions by controlling the sediment nitrogen contents, in which the ammonia nitrogen increased with increasing the level of water, while nitrate nitrogen decreased with increasing the level of water. The increase in the level of water significantly reduced the gene abundance of ammonia-oxidizing archaea (AOA), thereby reducing the N2O emissions in the MW and HW conditions due to the significant correlation between N2O emissions and AOA gene abundances. The unclassified_k_norank_d_Bacteria was the dominant denitrifying bacterial genus observed in the M. aquaticum ditches, and its highly relative abundance yielded low N2O emissions in the HW ditch. These findings indicate that reducing N2O emissions may be achieved by controlling the water level in vegetated ditches.