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Cardiovascular diseases (CVDs) such as stroke, high blood pressure, peripheral vascular disease, ischemic heart disease and acute myocardial infarction are some of the leading causes of death. To treat CVDs, commercially available thrombolytic agents are widely used. However, these thrombolytic agents have various side effects. Alternatively, fibrinolytic enzymes from bacterial sources are highly safe and have direct blood clot lytic activity.
A fibrinolytic enzyme producing bacterial strain, Bacillus flexus BF12, was isolated from a solar saltpan in Kanyakumari District, Tamilnadu, India. Enzyme production was improved by optimizing physical factors and nutritional factors.
A novel fibrinolytic enzyme was isolated from a strain of the studied B. flexus BF12. Enzyme production was enhanced significantly by optimizing process parameters. The critical physical factors (pH and salinity) and influencing nutritional factors (carbon, nitrogen and ions) were optimized by one variable at a time approach, followntials in nutraceutical and food fortification applications. The application of fibrinolytic enzymes could prevent CVDs.
The Bacillus flexus BF12 fibrinolytic enzyme shows promising potentials in nutraceutical and food fortification applications. The application of fibrinolytic enzymes could prevent CVDs.
Zanthoxylum bungeanum Maxim., a traditional Chinese herbal medicine, has been reported to possess therapeutic effects on diseases induced by oxidative stress (DOS), such as atherosclerosis and diabetes complication. However, the active components and its related mechanisms are still not systematically reported.
The current study was aimed to explore the main active ingredients and its molecular mechanisms of Z. bungeanum for treating DOS using network pharmacology combined with molecular docking simulation.
The active components of Z. bungeanum pericarps, in addition to the interacting targets, were identified from the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database. These components were filtered using the parameters of oral bioavailability and drug-likeness, and the targets related to DOS were obtained from the Genecards and OMIM database. Furthermore, the overlapping genes were obtained, and a protein-protein interaction was visualized using the STRING database. Next, the Cytoscapes of Z. bungeanum for treating DOS.
The findings of this study not only indicate the main mechanisms involving in the oxidative stress-induced diseases, but also provide the basis for further research on the active components of Z. bungeanum for treating DOS.
Given the rapidly increasing number of molecular biology data available, computational methods of low complexity are necessary to infer protein structure, function, and evolution.
In the work, we proposed a novel mthod, FermatS, which based on the global position information and local position representation from the curve and normalized moments of inertia, respectively, to extract features information of protein sequences. Furthermore, we use the generated features by FermatS method to analyze the similarity/dissimilarity of nine ND5 proteins and establish the prediction model of DNA-binding proteins based on logistic regression with 5-fold crossvalidation.
In the similarity/dissimilarity analysis of nine ND5 proteins, the results are consistent with evolutionary theory. Moreover, this method can effectively predict the DNA-binding proteins in realistic situations.
The findings demonstrate that the proposed method is effective for comparing, recognizing and predicting protein sequences. The main code and datasets can download from https//github.com/GaoYa1122/FermatS..
The findings demonstrate that the proposed method is effective for comparing, recognizing and predicting protein sequences. The main code and datasets can download from https//github.com/GaoYa1122/FermatS..
The use of nanoparticles has markedly increased in biomedical sciences. The silver nanoparticles (AgNPs) have been investigated for their applicability to deliver chemotherapeutic agents in cancer treatment. However, the existing chemical and physical methods of synthesizing AgNPs are considered inefficient and expensive, and are fraught with toxicity.
Natural products have emerged as viable candidates for nanoparticle production, including the use of Terfezia boudieri (T. boudieri), a member of the edible truffle family. Accordingly, our goal was to synthesize AgNPs using the aqueous extract of T. boudieri (green synthesized AgNPs). Since certain infectious agents are linked to cancer, we further investigated their potential as anti-cancer and antibacterial agents.
The physico-chemical properties of green synthesized AgNPs were analyzed by UV-Vis, FT-IR, XRD, SEM and TEM. find more In addition, their potential to inhibit cancer cell (MCF-7 and AGS) proliferation as well as the growth of infectious bacteria were investigated. Synthesis of AgNPs was confirmed by the presence of an absorption peak at 450nm by spectroscopy.
The size of nanoparticles ranged between 20-30nm and exerted significant cytotoxicity and bactericidal effects in a concentration and time dependent manner compared to T. boudieri extract alone. Interestingly, synthesis of smaller AgNPs correlated with longer synthesis time and enhanced cytotoxic and bactericidal properties.
This study shows that synthesis of smaller AgNPs correlated with longer synthesis time and enhanced cytotoxic and anti-bacterial effects.
This study shows that synthesis of smaller AgNPs correlated with longer synthesis time and enhanced cytotoxic and anti-bacterial effects.
This study aimed at investigating the gastro-protective effects of Algerian Sahara (Sidr) honey from Apis mellifera intermissa against HCl/Ethanol-induced gastric ulcers in rats.
Total phenolic content, antioxidant activity and phenolic compounds were determined. Than, Three groups of rats (control, HCl/ Ethanol induced ulcer, and orally administered honey) were used for the determination of gastro protective effect of Sidr honey.
Total phenolic content, total flavonoid content, and DPPH activity of honey sample was determined as 47.35±3.35 mg GAE/ 100 g, 2.13±0.17 mg QE/ 100 g, and 229.24±0.02 mg/mL, respectively. Oral pretreatment of rats with honey (1.2 g/Kg body weight orally at interval of 2 days) protected gastric mucosa against HCl/Ethanol induced damage by decreasing ulcer score, the volume and acidity of gastric juice and increasing pH.
These results were confirmed by the histological assessment which demonstrated a significant gastro protective activity of Saharian (Sidr) honey against HCl/Ethanol-induced stomach ulcer.
