Kjeldgaardirwin8111

85 ± 19.51µg/mL). Further, the flow-cytometric analysis revealed the increase in sub G1 population as well as early apoptotic populations dose-dependently. The results from confocal microscopy showed the DNA fragmentation in MCF-7 upon DCME treatment. Finally, the western blotting study revealed the induction of tumor suppressor protein, p53 which results in increasing Bax/Bcl-2 ratio and activation of caspase-cascade pathways. CONCLUSION The activation of caspase-3, -8, -9 and PARP degradation led us to conclude that DCME induces apoptosis in MCF-7 through both intrinsic and extrinsic mechanism. The LC-MS analysis showed the presence of various bioactive compounds. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.BACKGROUND Colorectal Cancer (CRC) is one of the most common fatal diseases with high morbidity. Alteration of the glucose metabolism is one of the hallmarks in the development of CRC. The Glucose Transporter 1 (GLUT1) is a key rate-limiting protein in hyperactive glucose metabolism and up-regulated in CRC, however, the underlying mechanism of the altered metabolism in CRC is still unknown. METHODS In this study, immunohistochemical staining was used to evaluate the expression of GLUT1 and FOXM1 in 135 paired CRC and adjacent normal tissues. The association between the expression of GLUT1/FOXM1 and clinicopathological factors was determined and the correlation between GLUT1 and FOXM1 in CRC was investigated. RESULTS Our results revealed that regardless of tumor location, GLUT1 and FOXM1 were overexpressed in CRC tissues, especially in patients with positive lymph node metastasis and TNM stage III-IV. Furthermore, GLUT1 showed a significantly strong link with FOXM1 in CRC tissue. CONCLUSIONS Overexpression of GLUT1 and FOXM1 may play critical roles in CRC that lead to a poor prognosis. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.BACKGROUND Xanthene doubted, but their derivatives have promising anticancer properties. OBJECTIVE To develop a few xanthene derivatives (a family of fifteen novel 3,4,6,7-tetrahydro-3,3- dimethyl-9-phenyl-2H-xanthene-1,8(5H, 9H)-diones encoded as 4a-4m), which were effectively prepared through regioselective synthesis approach and test their anticancer effects. METHODS A series of cell lines used in this study to assess the cytotoxicity initially and then the drug efficacy of target compounds, consecutively. Prior to MTT assay, the compounds subjected to estimate their antioxidant properties since oxidative stress is taking an important part in the development of many cancers. The anticancer properties of 4a-m assessed over in silico (molecular docking and ADMET assessments) and in vitro (MTT assay) methods. RESULTS Compounds 4h and 4i found to have established a relative percentage anticancer activity of 86.25±1.25 & 89.74±1.64 against BT474 (ER+HER2+), and 90.56±1.18 & 93.24±1.80 against MCF-7 (ER-HER2), respectively. CONCLUSION The need for the animal model and pre-clinical studies for 4h and 4i recognized in order to develop them as future anticancer agents. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.BACKGROUND Tectaria coadunata (T. coadunata) is an important fern species with a number of medicinal properties. It's been evidently found for its effectiveness in ethanomedicinal usage, which can also emerge as a one of the most promising sources for nutraceuticals. OBJECTIVE This study aims to examine the phytochemistry of the whole crude extract of T. coadunata for the first time with evaluation of antibacterial, antioxidant and anticancer activity. METHODS High Resolution Liquid Chromatography Mass Spectrometry analysis (HR-LCMS) was performed for confirming the presence of biologically active constituents in the extract of T. coadunata followed by antibacterial, antioxidant and anticancer activity. RESULTS With the detailed Mass spectra data, absorbance spectra and retention times; chemical composition of T. coadunata hold diverse group of bioactive/chemical components; sugars, sugar alcohol, flavonoids, terpenoids and phenolics. The results for antioxidant activity showed that T. coadunata crude extract had higher scavenging potential against 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals than H2O2 molecules, which was followed by positive antibacterial activity against several pathogenic bacteria; Shigella flexneri, Staphylococcus aureus and Salmonella typhi. The ethanolic extract of T. coadunata showed favourable antiproliferation activity against three leukemic (KG1, MOLT-3 and K-562) cells in a dose dependent manner, especially for KG142.850 ± 1.24µg/ml. CONCLUSION This study has provided a better understanding regarding the presence of biologically active phytochemical constituents in the extract of T. coadunata, which can be the reason for its bioactive potential. Moreover, T. ONO-7300243 research buy coadunata has significant anticancer activities against human leukemic cancer cell lines, indicating it a potential anticancer agent. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.Chronic obstructive pulmonary disease (COPD) and obstructive sleep apnoea syndrome (OSAS) are among the most prevalent chronic respiratory disorders. Accumulating data suggest that there is a significant burden of cardiovascular disease (CVD) in patients with COPD and OSAS, affecting negatively patients' quality of life and survival. Overlap syndrome (OS), i.e. the co-existence of both COPD and OSAS in the same patient, has an additional impact on cardiovascular system multiplying the risk of morbidity and mortality. The underlying mechanisms for the development of CVD in patients with either OSAS or COPD and OS are not entirely elucidated. Several mechanisms, in addition to smoking and obesity, may be implicated, including systemic inflammation, increased sympathetic activity, oxidative stress and endothelial dysfunction. Early diagnosis and proper management of these patients might reduce the cardiovascular risk and lead to improved survival. In this review, we summarize the current knowledge regarding epidemiological aspects, pathophysiological mechanisms and present point- to- point specific associations between COPD, OSAS, OS and components of CVD, namely, pulmonary hypertension, coronary artery disease, peripheral artery disease and stroke.