Keenekrogsgaard3955

suberin-related genes was induced by ShMYB78 heterologous expression in Nicotiana benthamiana leaves. ShMYB78 was shown to be a nuclear protein based on its presence in sugarcane internode nuclear protein extracts, and protoplast transactivation assays demonstrated that ShMYB78 activates the promoters of the sugarcane suberin biosynthetic genes β-ketoacyl-CoA synthase (ShKCS20) and caffeic acid-O-methyltransferase (ShCOMT). Our results suggest that ShMYB78 may be involved in the transcriptional regulation of suberin deposition, from fatty acid metabolism to phenylpropanoid biosynthesis, in sugarcane internodes.

The crop yield losses induced by phytoviruses are mainly associated with the symptoms of the disease. DNA modifications as methylation can modulate the information coded by the sequence, process named epigenetics. Viral infection can change the expression patterns of different genes linked to defenses and symptoms. CDDP in vivo This work represents the initial step to expose the role of epigenetic process, in the production of symptoms associated with plants-virus interactions. Small RNAs (sRNAs) are important molecules for gene regulation in plants and play an essential role in plant-pathogen interactions. Researchers have evaluated the relationship between viral infections as well as the endogenous accumulation of sRNAs and the transcriptional changes associated with the production of symptoms, but little is known about a possible direct role of epigenetics, mediated by 24-nt sRNAs, in the induction of these symptoms. Using different RNA directed DNA methylation (RdDM) pathway mutants and a triple demethylase mutant; elationship between viral infections as well as the endogenous accumulation of sRNAs and the transcriptional changes associated with the production of symptoms, but little is known about a possible direct role of epigenetics, mediated by 24-nt sRNAs, in the induction of these symptoms. link2 Using different RNA directed DNA methylation (RdDM) pathway mutants and a triple demethylase mutant; here we demonstrate that the disruption of RdDM pathway during viral infection produce alterations in the plant transcriptome and in consequence changes in plant symptoms. This study represents the initial step in exposing that DNA methylation directed by endogenous sRNAs has an important role, uncoupled to defense, in the production of symptoms associated with plant-virus interactions.Haematological malignancies induce important alterations of the immune system, which account for the high frequency of autoimmune complications observed in patients. Cutaneous immune-mediated diseases associated with haematological malignancies encompass a heterogeneous group of dermatoses, including, among others, neutrophilic and eosinophilic dermatoses, autoantibody-mediated skin diseases, vasculitis and granulomatous dermatoses. Some of these diseases, such as paraneoplastic pemphigus, are associated with an increased risk of death; others, such as eosinophilic dermatoses of haematological malignancies, run a benign clinical course but portend a significant negative impairment on a patient's quality of life. In rare cases, the skin eruption reflects immunological alterations associated with an unfavourable prognosis of the associated haematological disorder. Therapeutic management of immune-mediated skin diseases in patients with haematological malignancies is often challenging. Systemic corticosteroids and immunosuppressive drugs are considered frontline therapies but may considerably augment the risk of serious infections. Indeed, developing a specific targeted therapeutic approach is of crucial importance for this particularly fragile patient population. This review provides an up-to-date overview on the immune-mediated skin diseases most frequently encountered by patients with onco-haematological disorders, discussing new pathogenic advances and therapeutic options on the horizon.The first psychiatric chairs and university departments of psychiatry in Austria were founded in Vienna and Graz in 1870 hence they celebrate their 150th anniversary this year 2020. These foundations represent a peak in the development of psychiatry as a scientific medical discipline. It is the aim of this paper to sketch that step by step development in its connection with the development of care institutions of the time and with reference to important personalities in the field. Vienna-as the metropole of the state and the most prominent place in the field-is taken as an example. One can distinguish four developmental periods (1) The establishment of care institutions (asylums), at first without an own medical staff and unqualified male and female keepers (late 18th century up to early 19th). (2) The growing independance of these institutions-in Vienna the 'Tower of Fools'-and the emergence of 'alienists' who began to reflect on their activities and to share their experiences on academic grounds and the publication of a first Austrian textbook (1st half of 19th century). (3) From the 1850s on several alienists habilitated, partly with special emphasis on forensic psychology or cerebral anatomy and physiology, and developed a systematic academic teaching, again with a new 'modern' textbook, in some connection with a new care institution, and the foundation of a scientific association of psychiatry and forensic psychology in 1868. (4) An early activity of that association was to call for the institution of a chair and a university department of psychiatry within the asylum, both realised in 1870.The traditional approach to the diagnosis of primary adenohypophyseal cell proliferations uses hormone immunohistochemistry to classify pituitary neuroendocrine tumors (PitNETs). The routine application of immunolocalization of pituitary transcription factors (SF1, PIT1, TPIT, ERα, and recently GATA3) along with adenohypophyseal hormones has taught us critical lessons that are discussed in this communication. We point out that appropriate patient care requires accurate diagnosis and is critical in the era of precision medicine. A misdiagnosis can result in far greater health care costs than the cost of accurate tumor classification and may have other unintended consequences. We provide additional insights about confusing findings in genomic studies, emphasizing that high-quality pathology is essential for strong science and translational research.Neuroendocrine neoplasms (NENs) have traditionally been identified via expression of proteins associated to the regulation of secretory vesicles and granules. We report the clinical usage of the "second-generation" proteins ISL LIM homeobox 1 (ISL1), INSM transcriptional repressor 1 (INSM1), and secretagogin (SECG) as immunohistochemical markers of neuroendocrine differentiation since their introduction in clinical routine and compare the results with the established proteins chromogranin A (CGA) and synaptophysin (SYP). In total, 161 tumors, including 139 NENs and 22 "non-NENs" (unrelated tumors with an initial suspicion of NEN), were informatively stained for ISL1, and subsets were also interrogated for INSM1 and/or SECG. Diffuse or focal positive immunoreactivity was noted for ISL1 in 91/139 NENs (65%) and in 6/22 (27%) non-NENs, for INSM1 in 76/85 NENs (89%) and in 2/5 (40%) non-NENs, and for SECG in 49 out of 64 NENs (77%) and in 0/5 non-NENs (0%). Generally, ISL1, INSM1, and SECG exhibited sensitivities in line with or slightly below that of CGA and SYP-largely attributable to tissue-specific patterns regarding tumoral origin. Moreover, for pancreatic and small intestinal NENs, the two largest subgroups, ISL1 staining results were consistent irrespectively of tumor source and WHO grade. We verify previously suggested immunohistochemical schemes of neuroendocrine markers of first- and second-generations to facilitate the diagnostic process for NENs and confirm that the second-generation neuroendocrine markers display tissue-specific patterns. We therefore recommend their implementation in tertiary endocrine pathology centers, not least to aid in the identification of primary tumors when analyzing metastases.There are few stroke education programs focused on the South Asian population in the United States. The South Asian Health Awareness about Stroke (SAHAS) program was developed to provide culturally appropriate stroke education to South Asians from 2014 to 2017. Participants recruited for the SAHAS program were educated through a uniquely developed, culturally-specific, educational presentation. Each participant was asked to complete identical educational questionnaires both before (pre-) and after (post-) the intervention, which were then scored and evaluated. Overall, the 357 participants who completed the SAHAS program had a significant, modest 9% improvement in questionnaire score (p  60 (p  less then  0.0001). Having programs targeted and developed for specific minority groups with an emphasis on familial commitment and active participation may aid in raising awareness and reducing the elevated adverse stroke outcomes in South Asians.Wearable sensors are becoming increasingly popular in organizational research. Although validation studies that examine sensor data in conjunction with established social and psychological constructs are becoming more frequent, they are usually limited for two reasons first, most validation studies are carried out under laboratory settings. Only a handful of studies have been carried out in real-world organizational environments. Second, for those studies carried out in field settings, reported findings are derived from a single case only, thus seriously limiting the possibility of studying the influence of contextual factors on sensor-based measurements. This article presents a validation study of expressive and instrumental ties across nine relatively small R&D teams. The convergent validity of Bluetooth (BT) detections is reported for friendship and advice-seeking ties under three organizational contexts research labs, private companies, and university-based teams. link3 Results show that, in general, BT detections correlated strongly with self-reported measurements. However, the organizational context affects both the strength of the observed correlation and its direction. Whereas advice-seeking ties generally occur in close spatial proximity and are best identified in university environments, friendship relationships occur at a greater spatial distance, especially in research labs. We conclude with recommendations for fine-tuning the validity of sensor measurements by carefully examining the opportunities for organizational embedding in relation to the research question and collecting complementary data through mixed-method research designs.A new species of Acroeimeria Paperna & Landsberg, 1989 is described from the spotted house gecko, Gekko monarchus (Schlegel) from Peninsular Malaysia. Oöcysts of Acroeimeria grismeri n. sp. are spheroidal to subspheroidal with a smooth bi-layered wall, measure on average 18.4 × 17.3 µm, and have a length/width (L/W) ratio of 1.1; a micropyle and an oöcyst residuum are absent but variable polar granule(s) are present, commonly in Brownian movement. Sporocysts are ellipsoidal and measure on average 8.6 × 6.7 µm, L/W 1.3; Stieda, sub-Stieda and para-Stieda bodies are absent. The sporocyst residuum is composed of numerous spheroidal granules in the center of the sporocyst. This is the initial species of coccidian reported from G. monarchus and one of the few reported from any reptile from Peninsular Malaysia.