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23%; while the PCA-LDA model built using principal component variables achieved the best distinguish result with discrimination accuracy of 98.31%. It was concluded that SERS has the potential to be an inexpensive, rapid and effective method to detect and identify fungal species.The association between positive and negative attitudes and drug use profiles is examined. Participants were public middle school students (N = 5213; 49.9% girls; M age= 13.2 years) in three Brazilian cities. Analysis was performed using baseline surveys of a randomized controlled trial of the #Tamojunto2.0 drug use prevention program. We validated the attitudes construct via a confirmatory factor analysis and performed a simultaneous multinomial regression with a latent class analysis. Three latent classes were identified abstainers/low users (ABS) (63.4%), alcohol users/binge drinkers (ALC) (29.5%), and polydrug users (POL) (7.1%). An association gradient was found between attitudes and latent classes. Using ABS as reference, a direct association was identified between positive attitudes and ALC and POL. An inverse association was found between negative attitudes and POL only. Girls and older students were more likely to be ALC and POL. These findings, from a large probabilistic sample, provide guidance that drug use prevention programs should focus on deconstructing positive attitudes -mainly- and broadening negative attitudes, and should adapt their thematic content by considering the differential drug use possibilities among girls and older students.The unique shape of modern human feet, and how they change shape when loaded are thought to be integral to effective upright gait. This unique shape, and the natural variations therein, have previously been analysed using a range of methods; from visual assessments, anthropometric measurements, and footprints, to x-ray, ultrasound and magnetic resonance images. However, these methods are often limited by their use of linear two-dimensional measures. Only recently have advances in three-dimensional (3D) scanning technology and statistical shape analysis been applied to studying 3D foot shape variations. Given their novelty, information regarding the reliability and repeatability of 3D foot scanning and shape modelling is lacking. To investigate whether repeated foot scans captured by two examiners give the same 3D shape and produce consistent statistical shape models, 17 healthy adults' left feet were scanned while bearing half and full bodyweight, as well as minimal weight. Surface to surface distances between corresponding foot meshes and differences between shape model quality criteria were both found to be small and insignificant. The only exception being the specificity criterion for minimally loaded foot scans. Furthermore, Euclidean vectors were used to model the magnitude and direction of deformation that feet undergo as a consequence of increased loading. The deformation models showed that loading a minimally loaded foot results in greater, but less consistent, shape changes than when increasing the load on an already loaded foot. These results show that the utilized methods offer a valuable, reliable and repeatable approach to analysing foot shape and deformation.This study examined the validity of extended-field-of-view (EFOV) ultrasound imaging for evaluating the quantity (cross-sectional area [CSA]) and quality (accumulation of intramuscular fat) of trunk skeletal muscles (rectus abdominis, abdominal oblique and erector spinae) using magnetic resonance imaging (MRI) as a reference. Thirty healthy young men participated in this study. Cross-sectional images of the trunk at the height of the third lumbar vertebra were acquired and compared by EFOV ultrasound imaging and MRI. No significant differences were observed in CSAs between the two methods (0.74 ≤ R2 ≤ 0.85). Echo intensities significantly correlated with MRI-derived accumulation of intramuscular fat in each skeletal muscle group. However, the correlation coefficients were relatively low (0.37 ≤ r ≤0.47; p less then 0.05). These results indicate that EFOV ultrasound imaging is a reliable method for assessing trunk skeletal muscle CSA. Further research is warranted to find the optimal ultrasound setting for evaluating muscle quality.Left ventricular volumes (LVVs) and ejection fraction (LVEF) are key elements in the evaluation and follow-up of patients with heart failure with reduced ejection fraction (HFrEF). Therefore, a feasible and reproducible imaging method to be used by both experienced and in-training echocardiographers is mandatory. Our aim was to establish if, in a large echo lab, echocardiographers in-training provide feasible and more reproducible results for the evaluation of patients with HFrEF when using 3-dimensional echocardiography (3-DE) versus 2-dimensional echocardiography (2-DE). Sixty patients with HFrEF (46 males, age 58 ± 17 y) underwent standard transthoracic 2-D acquisitions and 3-D multibeat full volumes of the left ventricle. One expert user in echocardiography (expert) and three echocardiographers with different levels of training in 2-DE (beginner, medium and advanced) measured the 2-D LVVs and LVEFs on the same consecutive images of patients with HFrEF. Afterward, the expert performed a 1-mo training in 3-in echocardiography. In echo labs with a wide range of staff experience, 3-DE might be a more accurate method for the follow-up of patients with HFrEF.One day later, there was a marked increase in the vascular supply of the testis, and numerous vessels appeared to have breached the membrana propria of the tubules. Kochar NK, Harrison RG. The effects of x-rays on the vascularization of the mouse testis. Fertil Steril 1971;2253-57. The impact of temperature shock upon mammalian sperm is severe, and produces physical as well as biochemical alterations in the cells. Ackerman DR. Variation due to freezing in the citrate acid content of human semen. Fertil Steril 1971;2258-60.

To determine whether female body mass index (BMI) is associated with an increased risk of miscarriage after euploid embryo transfer.

A retrospective, observational, multicenter cohort study.

University-affiliated invitro fertilization center.

In this study, 3,480 cycles of invitro fertilization with preimplantation genetic testing for aneuploidy (PGT-A) in the blastocyst stage and euploid embryo transfer were divided into four groups according to patient BMI.

Invitro fertilization with PGT-A.

The primary outcome was the miscarriage rate, which included both biochemical and clinical miscarriages. Secondary outcomes were implantation, pregnancy, clinical pregnancy, and live birth rates.

Cycles were divided into four groups according to BMI (kg/m

) underweight (<18.5; n = 155), normal weight (18.5-24.9; n = 2,549), overweight (25-29.9; n = 591), and obese (≥30; n = 185). this website The number of PGT-A cycles per patient was similar in the four groups. Fertilization rate, day of embryo biopsy, technique of chromosomal analysis, number of euploid embryos, number of transferred embryos, and method of endometrial preparation for embryo transfer were similar in the four BMI groups.

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