Jessenstefansen8979

A modular approach to synthesizing functional pressure sensitive adhesives (PSAs) was introduced, wherein a modifiable acrylic PSA copolymer was synthesized by copolymerizing common PSA monomers with 6 mol % glycidyl methacrylate, allowing for subsequent functional group modification via the pendant epoxide functionality. This postmodification technique has the advantage of allowing the installation of a variety of functional groups relevant to adhesion, without variation of molecular weight. Because comparisons of cohesive and adhesive performance of candidate PSAs can be complicated by molecular weight differences, this strategy simplifies direct comparisons of the effects of functional groups on performance. As a proof of concept, a mussel-inspired catecholic PSA was synthesized by postreaction of the epoxide scaffold polymer with a thiol-modified catechol, allowing the effect of catechol on underlying structure-property relationships to be determined without variation in molecular weight. The mechanical performance of catecholic PSA was compared to relevant control PSAs by using industry-standard 180° peel and static shear tests, revealing an increase in peel strength achieved through catechol modification. Moreover, we observed an unexpected enhancement in PSA cohesive strength attributed to oxidation of catechol, which cannot be attributed to differences in molecular weight, a common source of changes in PSA cohesive strength.A novel series of well-defined dicarboxylate dinuclear nickel complexes containing benzotriazole based 1,3-diamine-bisphenolate (1,3-DiBTP) ligands were readily synthesized through a one-pot procedure, which were highly active single-component catalysts for copolymerization of CO2 and epoxides. X-ray structural determination of dinickel complexes 1-11 indicates that the DiBTP ligand acted as a N,O,N,N,O,N-hexadentate framework to chelate two nickel atoms, and two carboxylates are nonequivalently coordinated. The best benzoate-bonded dinickel catalyst 6 displayed the effective activity for both high-pressure and 1 atm CO2-copolymerization of cyclohexene oxide (CHO) in a controllable manner. Noteworthily, a high turnover frequency up to 9600 h-1 could be reached at 140 °C and a CO2 pressure of 20.7 bar utilizing a low catalyst loading of 0.01 mol %, and the same copolymerization conditions were capable of producing narrowly dispersed poly(cyclohexene carbonate) (PCHC) having >99% polycarbonate selectivity. In addition to CO2/CHO copolymerization, 4-vinyl-1,2-cyclohexene oxide or cyclopentene oxide was also applied to efficiently copolymerize CO2 under conditions of 80 °C and 20.7 bar initial CO2 pressure. Kinetic studies of CO2/CHO copolymerization catalyzed by 6 were investigated. Such polymerization revealed first-order dependence for both catalyst 6 and CHO concentrations, and the activation energy for PCHC generation by 6 is 57.69 kJ mol-1. A possible polymerization mechanism for CO2-copolymerization of CHO was proposed based on kinetics and structural studies of the obtained polycarbonates.Hydrogel materials with high water content and good biocompatibility are drawing more and more attention now, especially for biomedical use. However, it still remains a challenge to construct hydrogel fibers with enough strength and toughness for practical applications. Herein, we report a bio-inspired lotus-fiber-mimetic spiral structure hydrogel bacterial cellulose fiber with high strength, high toughness, high stretchability, and energy dissipation, named biomimetic hydrogel fiber (BHF). The spiral-like structure endows BHF with excellent stretchability through plastic deformation and local failure, assisted by the breaking-reforming nature of the hydrogen bonding network among cellulose nanofibers. With the high strength, high stretchability, high energy dissipation, high hydrophilicity, porous structure, and excellent biocompatibility, BHF is a promising hydrogel fiber for biomedicine. The outstanding stretchability and energy dissipation of BHF allow it to absorb energy from the tissue deformation around a wound and effectively protect the wound from rupture, which makes BHF an ideal surgical suture.Processivity is an important feature of enzyme families such as DNA polymerases, polysaccharide synthases, and protein kinases, to ensure high fidelity in biopolymer synthesis and modification. Here, we reveal processive character in the family of cytoplasmic protein N-glycosyltransferases (NGTs). Through various activity assays, intact protein mass spectrometry, and proteomics analysis, we established that NGTs from nontypeable Haemophilus influenzae and Actinobacillus pleuropneumoniae modify an adhesin protein fragment in a semiprocessive manner. Molecular modeling studies suggest that the processivity arises from the shallow substrate binding groove in NGT, which promotes the sliding of the adhesin over the surface to allow further glycosylations without temporary dissociation. We hypothesize that the processive character of these bacterial protein glycosyltransferases is the mechanism to ensure multisite glycosylation of adhesins in vivo, thereby creating the densely glycosylated proteins necessary for bacterial self-aggregation and adherence to human cells, as a first step toward infection.Pufferfish is a traditional, delicious dish in Asia. However, eating wild or improperly processed pufferfish causes serious poisoning. This study aimed to exploit the high-resolution melting (HRM) method for authenticating four species of Takifugu pufferfish (Takifugu xanthopterus, T. fasciatus, T. flavidus, and T. rubripes). Candidate DNA barcodes, including the cytochrome c oxidase subunit I (COI), cytochrome oxidase b (Cytb), and the control region (D-loop), were analyzed, with COI selected as the optimal DNA barcode. An HRM method was developed to identify 57 commercial fish samples in China, including 33 commercial pufferfish products and 24 unlabeled fish products. The findings revealed that the pufferfish products were T. rubripes or T. fasciatus, and four T. xanthopterus samples were detected in unlabeled fish products. JAK cancer These results showed that DNA barcode coupled with HRM analysis was a rapid and efficient tool to identify pufferfish, which might aid in the prevention of consumer fraud or mislabeling of fish products.