Jenkinsgrantham2510

nagement to wheat yield; adaptation measures may be more effective in planting areas where crop management contributes more, which will help stakeholders optimize crop management and adaptation strategies. © 2020 Society of Chemical Industry.

To report clinical, hormonal and structural effects of CYP11B1 pathogenic variations in Indian patients with 11β-hydroxylase deficiency (11βOHD) and find hormonal criteria that accurately distinguish 11βOHD from 21α-hydroxylase deficiency (21OHD).

Retrospective record review of genetically diagnosed patients with 11βOHD.

Clinical features, hormonal parameters at diagnosis (by immunoassay) and recent follow-up of 13 genetically proven 11βOHD patients managed at our centre were retrospectively reviewed. ACTH-stimulated serum adrenal steroids (measured by LC-MS/MS) of 11βOHD were compared with those of simple virilizing and non-classic 21OHD. Structural analysis of the observed pathogenic variations was performed by computational modelling.

Nine (four females) and four (all females) patients had classic and non-classic disease, respectively. All 11βOHD patients had elevated ACTH-stimulated serum 11-deoxycortisol (26.5-342.7nmol/L) whereas none had elevated serum 17-hydroxyprogesterone (4.2-21.2nmol/L); bight novel pathogenic variants were identified in our study, highlighting regional genetic heterogeneity. Measurement of ACTH-stimulated adrenal steroids by LC-MS/MS will help avoid the misdiagnosis of 11βOHD as 21OHD and has potential to distinguish classic from non-classic 11βOHD.Using attachment theory as the framework, this qualitative study focused on the romantic relationship experiences of regular mindfulness meditators and their meditation-naïve or nonregularly meditating partners (NMPs). The sample consisted of eight heterosexual and two same-sex couples. Thematic analysis (TA) yielded seven themes on three levels. Three intrapersonal themes were (a) meta-awareness of one's emotional strategies; (b) shift in emotion regulation strategies; and (c) shift in relationship with oneself. The relationship-level themes of meditators emerged as (d) experiential shift in stressful situations; (e) transformation of the emotional bond; and (f) transformation of the romantic relationship; the nonmeditating partners confirmed the relational and individual shifts due to their partner's practice. Integrating attachment theory with meditation practice can thus be a valuable approach for couple and family therapy. A regular meditation practice of even just one of the partners may be beneficial for the couple relationship.

Wnt/β-catenin signalling, in the microenvironment of pluripotent stem cells (PSCs), plays a critical role in their differentiation and proliferation. Contradictory reports on the role of Wnt/β-catenin signalling in PSCs self-renewal and differentiation, however, render these mechanisms largely unclear.

Wnt/β-catenin signalling pathway in human-induced pluripotent stem cells (hiPSCs) was activated by inhibiting glycogen synthase kinase 3 (GSK3), driving the cellsinto a mesodermal/mesenchymal state, exhibiting proliferative, invasive and anchorage-independent growth properties, where over 70% of cell population became CD 44 (+)/CD133 (+). Wnt/β-catenin signalling activation also altered the metabolic state of hiPSCs from aerobic glycolysis to oxidative metabolismand changed their drug and oxidative stress sensitivities.These effects of GSK3inhibition were suppressedinHIF1α-stabilised cells.

Persistentactivation of Wnt/β-catenin signalling endows hiPSCs with proliferative/invasive 'teratoma-like'states, shifting their metabolic dependence and allowing HIF1α-stabilisation to inhibit their proliferative/invasive properties.

The hiPSC potential to differentiate into 'teratoma-like' cells suggest that stem cells may exist in two states with differential metabolic and drug dependency.

The hiPSC potential to differentiate into 'teratoma-like' cells suggest that stem cells may exist in two states with differential metabolic and drug dependency.The proliferation of misuse of prescription and non-prescription opioids, in recent years, has caused an opioid epidemic in the United States. Selleck 2-MeOE2 Forensic toxicology laboratories often encounter implications of abuse in both driving under the influence of drugs and death investigation cases. The Virginia Department of Forensic Science has seen over a 190% increase in the number of reported opioids in death investigation cases over the past several years. Traditionally, analyses are completed by individual drug class, which subsequently requires an individual case to be evaluated using multiple analytical techniques for comprehensive analysis. To ease the impact of increasing case submissions and case complexity, two liquid chromatography-tandem mass spectrometry (LCMSMS) methods for the quantitative analysis of opioids, cocaine, and cocaine metabolites in biological matrices have undergone a fit-for-purpose validation. The methods were compared to determine the advantages and disadvantages of each analytical technique. Two sample preparation techniques, protein precipitation and solid-phase extraction, were employed for quantitative validation. Validation aspects evaluated included accuracy and precision, sensitivity, linearity, matrix effects, recovery, carryover, interferences, dilution integrity, and post-extraction stability. The accuracy of the protein precipitation method ranged from 80 ± 3% to 101 ± 10%. The accuracy of the solid-phase extraction method ranged from 88 ± 9% to 119 ± 3%. Additionally, within-run and intermediate precision was within ±20% for both extraction techniques. Although ionization suppression was noted, it was determined to have minimal effect on the methods. The newly developed methods require less sample volume and combine four analytical techniques into one method, which significantly impacts laboratory productivity.

Glycidyl esters (GEs) have attracted worldwide attention for their potential harm to human health. The GEs in edible oils mainly form during the deodorization of the oil refining processes. We used sesamol and sesamolin to inhibit the formation of GEs in model corn oil (MCO), model palm oil (MPO) and model rice bran oil (MRO) during a deodorization process.

The results showed that, in the three model oils, the total GE content was in the following order from highest to lowest MRO (1437.98 μg kg

) > MPO (388.64 μg kg

) > MCO (314.81 μg kg

). The inhibitory effect of the three antioxidants on the formation of GEs in the MCO was in the following order from strongest to weakest tert-butylhydroquinone (TBHQ) > sesamol > sesamolin.

When the mass percentage of sesamol was 0.05%, its inhibition percentage on GEs was close to the inhibition percentage of 0.02% added TBHQ. The present study provides a foundation for understanding how to inhibit the formation of GEs in oils by adding sesamol during the deodorization process.