Ipsenstewart4444

The aim To establish the role of allelic polymorphisms NOS3-T-786C, MTHFR-C667T, P2RY12--744C, (GPIbα)-C482T in the development of vascular lesions in patients with hypertension and diabetes mellitus type 2.

Materials and methods The study included 100 patients with hypertension and diabetes mellitus type 2 (main group) and 50 patients without type 2 diabetes (control group). Patients underwent echocardiography, color duplex scanning of extracranial, brachiocephalic and femoral vessels. The distribution of allelic polymorphisms was investigated by isolation DNA from leukocytes and polymerase chain reaction (PCR).

Results The risk of vascular damages increases 2-fold when carrying all 4 risk alleles in monozygotic genotypes of polymorphic loci in patients with hypertension with concomitant type 2 diabetes (p<0,05). In gene-gene interaction, the values of contributions and directions of interaction between alleles of polymorphic loci are established (p<0,05). Genes create a paired hierarchy of interaction according to their functional activity; the largest contribution to the probable vascular damage depends on the allelic polymorphism NOS3-786CT (p<0,05), the lowest - on the allelic polymorphism P2RY12-744CC (H2H2). The genetic polymorphism of the MTHFR gene is independent of the influence of other studied polymorphisms (p<0,05); the genes P2RY12-744CT and GPIbα 482CT act synergistically with the gene NOS3-786CT, being in a weak negative interaction with each other.

Conclusions Phenotypic manifestations of endothelial dysfunction may be modified by allelic polymorphism of genes associated with endothelial and platelet functions with the risk of vascular complications.

Conclusions Phenotypic manifestations of endothelial dysfunction may be modified by allelic polymorphism of genes associated with endothelial and platelet functions with the risk of vascular complications.

The aim The objective of our study was to evaluate the features of ultramorphometric characteristics of exocrine parenchyma and microvasculature of the pancreas in the presence of moderate dehydration by means of an experiment in laboratory rats.

Materials and methods The experiment involved 20 mature white male rats divided into 2 groups control and experimental (10 rats each). In the experimental group, moderate dehydration was simulated, i.e. the animals were deprived of water for 7 days, while the control rats were provided with a normal water supply during the study. Pancreatic parenchyma samples were fixed in phosphate-buffered glutaraldehyde solution and post-fixed in osmium tetroxide solution, dehydrated and embedded in a mixture of epoxy resins. Ultrastructural analysis was performed using JEOL JEM-1230 transmission electron microscope (Japan).

Results Pancreatic electron microscopy in the presence of moderate dehydration demonstrated statistically significant changes in exocrinocytes area and exocrinocyte nucleus area which increased by 8.02% (p = 0.028) and 40.28% (p < 0.001), respectively. Among the vessels of microcirculation, the largest changes occurred in the capillaries their lumen narrowed by 22.34% (p = 0.002) as compared with the control group. The cytoplasm of endothelial cells contained a large number of vacuoles and micropinocytotic vesicles.

Conclusions Among the organelles of exocrinocytes, mitochondria appeared the most vulnerable to the effects of dehydration. They demonstrated polymorphic changes a part of the mitochondria was hypotrophic and had partially reduced cristae, and another part was hypertrophic.

Conclusions Among the organelles of exocrinocytes, mitochondria appeared the most vulnerable to the effects of dehydration. They demonstrated polymorphic changes a part of the mitochondria was hypotrophic and had partially reduced cristae, and another part was hypertrophic.

The aim To determine the difference between body composition and hemodynamics indices at baseline and after the weight loss program.

Materials and methods The subject of this study was 13 young women. The weight and body composition were measured by the bio-impedance method. Hemodynamics indices were measured by the method of the thoracic rheography. The measurement of body composition and indices of hemodynamics were performed at the beginning of the weight loss program and 2 months later. The participants underwent 45 minutes per day of moderate-intensity physical activity 3 times a week.

Results The percentage of body fat decreased in 3.9±0.37% from baseline (p=0.01) and the level of visceral fat - 1.54±0.14 units (p=0.001) respectively. Indices of hemodynamics were improved after the weight loss program. Firstly, the index of cardiac output was reduced in 1.43±1.09 l/min (p=0.019) after 2 months of the weight loss program. Secondly, the indices of peripheral resistance also have been improved. Moreover, the index of workload of left ventricle has decreased from 3.56 to 2.7 kg/m/m2 (p=0.035).

Conclusions Our results demonstrated the improvement of indices of hemodynamics due to the normalization of body composition among young women after weight loss program.

Conclusions Our results demonstrated the improvement of indices of hemodynamics due to the normalization of body composition among young women after weight loss program.

The aim Determination of circulation interrelations between antibiotic-resistant microorganisms of Enterobacteriaceae family and their resistance genes in clinical strains and potable water samples taken in Uzhhorod and Uzhhorod district.

Materials and methods We carried out generic identification of the microorganisms isolated from clinical samples of the oral cavity of 64 patients suffering from periodontal inflammatory diseases, and potable water samples taken from sources of public centralized and decentralized water supply; the isolated microorganisms were tested for antibiotic sensitivity by the Kirby-Bauer disc diffusion method according to EUCAST. With the help of molecular-genetic methods, the total DNA of potable water was isolated and tested for the presence of the following genetic resistance determinants carbapenems blaNDM; blaOXA48-like; tetracyclines blaTet-M; cephalosporins blaCTX-M.

Results In the microbiota of the clinical material and potable water samples, the same spectrum of microorganisms belonging to Enterobacteriaceae family dominated; the isolated bacteria showed a high resistance level to beta-lactam antibiotics and to natural antibiotic preparations. The highest level of resistance was established for microorganisms isolated from well water samples, where genetic resistance determinants to blaCTX-M cephalosporins and blaTet tetracyclins were also revealed.

Conclusions The obtained results proved high probability of the spread of antibiotic-resistant microorganisms and their genetic resistance determinants via potable water.

Conclusions The obtained results proved high probability of the spread of antibiotic-resistant microorganisms and their genetic resistance determinants via potable water.

The aim To determine the features of changes in serum ghrelin levels and its relationship with the body mass index in patients with GERD and spondyloarthritis (SpA) with lesions of the cervical and thoracic spine.

Materials and methods The examined patients included 80 patients with SpA with cervical and thoracic spine lesions in combination with GERD. The examined patients with SpA with predominant cervical and thoracic spine lesions were divided into two groups depending on the clinical course of GERD, namely group I included 33 (41.2%) patients with typical esophageal manifestations of GERD (13 males (39.4%), 20 females (60.6%)), and group II consisted of 47 (58.8%) patients with atypical extraesophageal manifestations of GERD (among them were 17 (36.2%) males and 30 (63.2%) females).

Results All patients were tested for serum ghrelin by enzyme-linked immunosorbent assay. Analysis of clinical manifestations of atypical GERD (group II patients) revealed that most often patients with SpA of the cervicalevels was found, namely in group II patients with overweight and obesity a direct correlation was found, and in group I patients with underweight an inverse correlation was fund.

Conclusions 1. In patients with SpA with cervical and thoracic spine lesions, GERD often has atypical symptoms (mostly dental and otolaryngological forms in 40.4% and 25.5% of patients). 2. check details In patients with SpA with esophageal clinical signs of GERD, normal weight or underweight is more common, while in patients with extraesophageal forms of GERD overweight or obesity of varying severity prevails. 3. In patients with SpA and GERD, an increase in serum ghrelin levels was found in patients with cardiac manifestations of reflux disease (355.02 ± 4.75 ng/ml). 4. The relationship between BMI changes in patients with SpA and GERD and increased serum ghrelin levels was found, namely in group II patients with overweight and obesity a direct correlation was found, and in group I patients with underweight an inverse correlation was fund.

The aim To investigate the status and possibilities of markers of the inflammatory response of organism in infants with identified IUI born to mothers diagnosed with TORCH infection.

Materials and methods The study group included infants diagnosed with IUI (n = 40), born to mothers (age 31.31 ± 2.08 years) with the diagnosis of TORCH infection and a control group (n = 25 infants). Childbirth in all newborns was physiological. The average weight of newborns was 1877.69 ± 981.78 g (min - 600 g; max - 4000 g). Gestational age 32.25 ± 5.15 weeks. Observation and treatment of newborns lasted up to 7 days (included stay in the emergency department of the Uzhhorod maternity hospital in the Zakarpattia region). Cytokine profile, γ-IFN, TNF-α, Pg E2, serum neopterin and procalcitonin levels were studied.

Results The values of the parameters of the cytokine profile (IL-1, IL-6, IL-8, IL-10) varied within the reference values, but with significant differences with the values of the control group, which was 1,2; 4;erefore curent changes in infectious circumstances make new demands on research. It has been proven that intrauterine infection has a negative effect on the homeostatic parameters of infants, in particular, on the indicators of the inflammatory response of the child's organism. Symptomatic inflammatory biomarkers can be used to identify the pathological condition of the infant, in addition to routine laboratory tests, for early correction of VUI. This delay in identifying affected infants can lead to long and unnecessary therapy, the emergence of resistant strains of microorganisms, increased treatment costs and, in particular, a higher risk of complications such as cerebral palsy or intraventricular hemorrhage.

The aim To investigate the effectiveness of complex protocol treatment with the additional inclusion of a course of the sublingual form of hepatoprotector on the clinical manifestations of patients with chronic pancreatitis in combination with type 2 diabetes mellitus.

Materials and methods We studied 57 outpatients with chronic pancreatitis in the phase of stable or unstable remission in combination with diabetes mellitus in the phase of stable or unstable remission. Two groups were formed according to randomization principles to study the effectiveness of the proposed correction programs 1stgroup (30 patients) took protocol treatment for one month, 2nd group (27 patients) - received protocol treatment with a course of hepatoprotector.

Results It was found the results of the impact of two treatment programs on some clinical symptoms and syndromes in patients with chronic pancreatitis. Positive dynamics of clinical symptoms/syndromes were found in both groups of patients, but the therapeutic effect in the 2nd group was more significant.