Hyllestedglud1177
Nano-chitosan (NCH), nano-cellulose (NCL) and cellulose derivative are biodegradable biopolymer. Nano-chitosan or nano-cellulose at different concentrations (0.1, 0.5 and 1%) incorporated in carboxymethyl cellulose (CMC) film solution using casting methods. Both CMC/NCH and NCL decreased physical properties (water solubility, moisture content and moisture absorption) especially in concentration of 1%. However, these properties in CMC/NCH were significantly (p less then 0.05) lower than CMC/NCL. Water vapor permeability of polymer and nanofiller decreased when nanocomposite concentration increased. Tensile strength and Elongation at break improved in nanocomposite film by increasing concentration. Thermal properties of CMC/NCH were significantly (p less then 0.05) lower than CMC/NCL. Emersion of crystalline peaks in X-ray analyses certified the presence of nanofiller in polymer. However, in high content (1%) cause to create aggregation of nanofiller in CMC film. Finally antibacterial activity against five pathogens was studied and good effective inhibition on CMC/NCH was observed while CMC/NCL had no inhibitory effect. These results show that use of CMC/NCH as a biocompatible film has more advantages than CMC/NCL biopolymer.The aim of the paper was to investigate the ability of an eco-friendly luminescent xerogel prepared by chitosan crosslinking with a phenothiazine luminogen to detect and remove heavy metals. Its ability to give a divergent morphological and optical response towards fifteen environmental relevant metals was investigated by naked eye and UV lamp, fluorescence spectroscopy and scanning electron microscopy. A distinct response was noted for mercury, consisting in the transformation of the xerogel into a rubber-like material accompanied by the red shifting of the color of emitted light from yellow-green to greenish-yellow domain. The particularities of the metals anchoring into the xerogel were analyzed by FTIR spectroscopy and X-ray diffraction. The morphological changes and the metal uptake were analyzed by SEM-EDAX, swelling and gravimetric methods. It was concluded that mercury has a superior affinity towards this heteroatoms rich system, leading to a secondary crosslinking. This directed a great absorption capacity of 1673 mg/g and a specific morphological response for mercury ion concentrations up to 0.001 ppm.We herein report the synthesis of CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) via a greener method followed by sodium alginate (SA) passivation and encapsulation into mesoporous channels of amine modified silica (SBA15-NH2) for improved photostability and biocompatibility. The as-synthesized CIS/ZnS QDs exhibited near infrared emission even after SA passivation and silica encapsulation. Transmission electron microscopy (TEM) and Small angle X-ray diffraction (XRD) revealed the mesoporous nature of the SBA-15 remained stable after loading with the SA-CIS/ZnS QDs. The effective encapsulation of SA-CIS/ZnS QDs inside the pores of SBA15-NH2 matrix was confirmed by Brunauer-Emmett-Teller (BET) pore volume analysis while the interaction between the QDs and SBA15-NH2 was confirmed using Fourier transform infrared (FTIR) spectroscopy. The photostability of the QDs was greatly enhanced after these modifications. The resultant SA-CIS/ZnS-SBA15-NH2 (QDs-silica) composite possessed remarkable biocompatibility towards lung cancer (A549) and kidney (HEK 293) cell lines making it a versatile material for theranostic applications.The global health emergency generated by coronavirus disease 2019 (COVID-19) has prompted the search for preventive and therapeutic treatments for its pathogen, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). There are many potential targets for drug discovery and development to tackle this disease. One of these targets is the main protease, Mpro or 3CLpro, which is highly conserved among coronaviruses. 3CLpro is an essential player in the viral replication cycle, processing the large viral polyproteins and rendering the individual proteins functional. We report a biophysical characterization of the structural stability and the catalytic activity of 3CLpro from SARS-CoV-2, from which a suitable experimental in vitro molecular screening procedure has been designed. By screening of a small chemical library consisting of about 150 compounds, the natural product quercetin was identified as reasonably potent inhibitor of SARS-CoV-2 3CLpro (Ki ~ 7 μM). Quercetin could be shown to interact with 3CLpro using biophysical techniques and bind to the active site in molecular simulations. Quercetin, with well-known pharmacokinetic and ADMET properties, can be considered as a good candidate for further optimization and development, or repositioned for COVID-19 therapeutic treatment.To promote the application of probiotics that are beneficial to hosts, calcium-alginate (Ca-Alg) coated whey protein isolate microcapsules were prepared for protection and delivery of L. bulgaricus and L. paracasei. The internal layer was formed with transglutaminase-induced gelation of whey protein isolate (WPI). Sodium alginate (SA) was applied to form outer layer with external Ca2+ gelation method. The microcapsules loaded with probiotics were characterized by scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FT-IR), and differential scanning calorimetry (DSC). The results showed that the co-encapsulation efficiency was 90.54% and 84.46% of WPI micro-beads and Ca-Alg-coated microcapsules, respectively. The trehalose was added as cryoprotectant to improve the survival rate of probiotics in freeze-dried Ca-Alg-coated microcapsules from 3% to 41.26%. Ca-Alg-coated microcapsules have regular morphology and intensified structure. The protection of Ca-Alg-coated microcapsule for probiotics was improved under simulated gastrointestinal and thermal conditions. Ca-Alg-WPI microcapsules showed a useful way for protection and delivery of L. bulgaricus and L. #link# paracasei.
To determine whether ballistic resistance training is feasible, safe, and effective in improving muscle strength, power generation, and mobility in adults with neurologic conditions.
Nine electronic databases were searched from inception to March 2019 in addition to the reference lists of included articles.
Articles were independently screened by 2 authors and were included if they were full-text; English-language articles published in a peer-reviewed journal; investigated ballistic resistance training for adults with a neurologic condition; and reported on feasibility, safety, strength, power, or mobility.
Two authors independently extracted data. Study quality was assessed using the McMaster critical review form and the Physiotherapy Evidence Database scale.
The search identified 1540 articles, with 13 articles describing 9 studies meeting the criteria for inclusion. Five studies were randomized controlled trials and 4 were cohort studies. Ballistic resistance training was feasible and safe with oe found to be positive but not conclusive.
To examine the effect of agitation, cognitive impairment, fatigue, and pain on physical therapy participation and outcomes during posttraumatic amnesia (PTA) after traumatic brain injury (TBI).
Prospective longitudinal study.
Inpatient rehabilitation hospital.
Participants (N=77) with moderate-to-severe TBI who were deemed to be experiencing PTA using the Westmead Post-Traumatic Amnesia Scale.
Not applicable.
The Pittsburgh Rehabilitation Participation Scale and time in therapy (min) were recorded twice daily after routine physical therapy sessions during PTA. The FIM-motor (select items related to physical therapy) score rated on admission and after emergence from PTA was used to calculate FIM-motor change.
selleck compound was associated with lower participation in therapy. The presence of agitation and pain both predicted lower FIM-motor change at emergence from PTA. Higher levels of cognitive impairment and fatigue were also associated with lower participation and less time in therapy.
The presenpromoting cognitive recovery and arousal during PTA to maximize physical gains. Further research is warranted to examine the factors associated with rehabilitation success across other therapeutic disciplines.
To summarize the effectiveness of physical therapy interventions to reduce fear of falling (FOF) among individuals living with neurologic diseases.
PubMed, Physiotherapy Evidence Database, Scopus, Web of Science, PsycINFO, Cumulative Index to Nursing and Allied Health, and SportDiscuss were searched from inception until December2019.
Clinical trials with either the primary or secondary aim to reduce FOF among adults with neurologic diseases were selected.
Potential articles were screened for eligibility, and data were extracted by 2 independent researchers. Risk of bias was assessed by the Cochrane Risk of Bias tool for randomized controlled trials and the National Institutes of Health Quality Assessment Tool for pre-post studies. A meta-analysis was performed among trials presenting with similar clinical characteristics. The Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) was used to rate the overall quality of evidence.
Sixty-one trials with 3954 participants were incluHowever, because of several limitations of the included studies, further research is needed to examine the effectiveness of FOF intervention among individuals with neurologic diseases.
Gait with lower limb training combined with balance training is effective in reducing FOF in individuals with PD. Also, home-based or leisure exercise is effective among individuals with MS. link2 However, because of several limitations of the included studies, further research is needed to examine the effectiveness of FOF intervention among individuals with neurologic diseases.With the rapid development of human's exploitation of nature and animal husbandry, zoonoses have become a major public health problem worldwide. It is necessary to establish a rapid, specific and sensitive detection method to screen several zoonotic pathogenic bacteria simultaneously. In this study, phage display technology was used to screen specific peptide of three common zoonotic pathogens, E. coli O157H7, L. monocytogenes and B. melitensis 16 M. Then, three peptide were obtained, named E2, L4 and B4, which can identify the three pathogens respectively. Three peptide modified with biotin were synthesized and were coupled to streptavidin magnetic beads (MBs) to form peptide-MBs, which enriched the above three pathogens from the samples. Three quantum dot (QD) probes were constructed by coupling three polyclonal antibodies to different fluorescent QD surfaces (QD540, QD580 and QD630). The simultaneous detection method based on peptide-MBs and QDs multicolor fluorescent labeling was established and could detect E. coli O157H7, L. monocytogenes and B. melitensis 16 M simultaneously. The detection method took about 100 min with the detection limits of 103, 102 and 102 CFU/mL, respectively. link3 The detection method could be also well utilized in real samples.The transfection of synthetic small interfering (si)RNA into cultured cells forms the basis of studies that use RNA interference (commonly referred to as "gene knockdown") to study the impact of loss of gene or protein expression on a biological pathway or process. In these studies, mock transfections (with transfection reagents alone), and the use of synthetic negative control (apparently inert) siRNA are both essential negative controls. This report reveals that three widely-used transfection reagents (X-tremeGENE™, HiPerFect, and Lipofectamine® 2000) and five commercially-available control siRNA (from Ambion, Sigma, Santa Cruz, Cell Signaling Technology, and Qiagen) are not inert in cell-culture studies. Both transfection reagents and control siRNA perturbed steady-state mRNA and protein levels in primary mouse lung fibroblasts and in NIH/3T3 cells (a widely-used mouse embryonic fibroblast cell-line), using components of the canonical transforming growth factor-β signaling machinery as a model system. Furthermore, transfection reagents and control siRNA reduced the viability and proliferation of both lung fibroblasts and NIH/3T3 cells.
