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The enhanced toxicity under field conditions is at least partially due to activation of colonial nutritional responses missed by lab tests. Pristine® causes colonies to respond to perceived protein malnutrition by increasing colony pollen collection. Additionally, Pristine induces much earlier transitioning to foraging in individual workers, which could be the cause of shortened lifespans. These findings demonstrate that Pristine® can negatively impact honey bee individual and colony health at concentrations relevant to what they experience from pollination behavior under current agricultural conditions.To meet human food and fiber needs in an environmentally and economically sustainable way, we must improve the efficiency of waste, water, and nutrient use by converting vast quantities of agricultural and food waste to renewable bioproducts. This work converts waste cherry pits, an abundant food waste in the Great Lakes region, to biochars and activated biochars via slow pyrolysis. Biochars produced have surface areas between 206 and 274 m2/g and increased bioavailability of Fe, K, Mg, Mn, and P. The biochars can be implemented as soil amendments to reduce nutrient run-off and serve as a valuable carbon sink (biochars contain 74-79% carbon), potentially mitigating harmful algal blooms in the Great Lakes. CO2-activated biochars have surface areas of up to 629 m2/g and exhibit selective metal adsorption for the removal of metals from simulated contaminated drinking water, an environmental problem plaguing this region. XL177A molecular weight Through sustainable waste-to-byproduct valorization we convert this waste food biomass into biochar for use as a soil amendment and into activated biochars to remove metals from drinking water, thus alleviating economic issues associated with cherry pit waste handling and reducing the environmental impact of the cherry processing industry.Copper-based (nano)pesticides in agroecosystems may result in unintended consequences on non-target soil microbial communities, due to their antimicrobial broad spectrum. We studied the impact of a commercial Cu(OH)2-nanopesticide, over 90 days, at single and season agricultural application doses, in the presence and absence of an edaphic organism (the isopod Porcellionides pruinosus), on microbial communities' function, structure and abundance. Results were compared to the effects of Cu(OH)2-ionic. The nanopesticide application resulted in significant changes on both bacterial and fungal communities' structure, particularly at the season application. The exposed bacterial community presented a significantly lower richness, and higher diversity and evenness while the exposed fungal community presented lower diversity and richness. At the functional level, a significant increase on microbial ability of carbon utilization and a significant decrease on the β-glucosidase activity was observed for communities exposed to the nanopesticide. Regarding Cu forms, less pronounced effects were observed in soils spiked with Cu(OH)2-ionic, which might result from lower Cu concentration in porewater. The presence of P. pruinosus did not induce significant changes in diversity indexes (fungal community) and community-level physiological profiling, suggesting an attenuation of the nanopesticide effect. This study revealed that Cu(OH)2-nanopesticide, at doses applied in agriculture, impact the soil microbial community, possibly affecting its ecological role. On the other hand, invertebrates may attenuate this effect, highlighting the importance of jointly including different interacting communities in the risk assessment of nanopesticides in soils.In Europe, emerging organic compounds (EOCs) in groundwater is a growing research area. Prioritisation for monitoring EOCs in Europe was formalised in 2019 through the development of the first voluntary groundwater watch list (GWWL). Despite this, groundwater occurrence data in the peer reviewed literature for Europe has not been reviewed to date. Questions surrounding the effect, toxicity, movement in the subsurface and unsaturated zone make the process of regulating EOC use difficult. The aim in Europe is to develop a unified strategy for the classification, and prioritisation of EOCs to be monitored in groundwater. This paper compiles evidence from the recent published studies from across Europe, since 2012, when the last major literature global review of EOCs in groundwater took place. A total of 39 studies were identified for review based on specific selection criteria (geography, publication date, sample size>10, inclusion of EOCs data). Data on specific compounds, and associated meta-data, are compiled and reviewed. The two most frequently detected EOCs, carbamazepine and caffeine, occurred in groundwater at concentrations of up to 2.3 and 14.8 μg/L, respectively. The most frequently reported category of compounds were 'Pharmaceuticals'; a highly studied group with 135 compounds identified within 31 of the 39 studies. In Europe, the majority of reviewed studies (23) were at a regional scale, looking specifically at EOCs in a specific city or aquifer. The use of analytical methods is not uniform across Europe, and this inevitably influences the current assessment of EOCs in groundwater. A correlation between the number of compounds analysed for, and the number detected in groundwater highlights the need for further studies, especially larger-scale studies throughout Europe. For the development of EU and national regulation, further work is required to understand the occurrence and impacts of EOCs in groundwater throughout Europe and elsewhere.Diazinon is a common organophosphate pesticide widely used to control parasitic infections in agriculture. Excessive use of diazinon can have adverse effects on the environment and aquatic animal health. In the present study, the toxic effects of diazinon on the histology, antioxidant, innate immune and intestinal microbiota community composition of crucian carp (Carassius auratus gibelio) were investigated. The results showed that diazinon at the tested concentration (300 μg/L) induced gill and liver histopathological damages. Hepatic total superoxide dismutase (T-SOD), catalase (CAT), and glutathione S-transferase (GST) activities significantly decreased (P less then 0.05) by 32.47%, 65.33% and 37.34%, respectively. However, the liver tissue malondialdehyde (MDA) content significantly (P less then 0.05) increased by 138.83%. The 300 μg/L diazinon significantly (P less then 0.05) downregulated the gene expression of TLR4, MyD88, NF-kB p100 and IL-8 but had no significant effect TNF-α (P = 0.8239). In addition, the results demonstrated that diazinon exposure could affect the intestinal microbiota composition and diversity.

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