Hopperlindsay6726

A growing demand exists on the monitoring of both uranium mining activities and their environmental impacts. In order to help understanding and modelling both these aspects, a thermodynamic database dedicated to uranium mining activities is developed the PRODATA database. Relevant species and phases for uranium and radium are chosen from existing compilations of data, complemented with important missing data for the application to mining activities and environmental monitoring. Important major anions and cations chemistry are included, as well as secondary pollutants such as arsenic, lead, or nickel. Applications of the PRODATA extracted database file for PhreeqC to theoretical speciation calculations of uranium and radium for actual water compositions - either linked to uranium mining activities, or under monitoring for environmental survey - are presented. Wider applications to other available water compositions from different geochemical concepts are also tested. For the tested cases, the major radium and uranium species obtained using PRODATA are compared with other available thermodynamic database (Thermochimie, LLNL, Wateq4f, Minteq, PSI/NAGRA). The choice of the database file - and of the ionic strength correction - can strongly impact the final speciation results. Sulphate complexes of radium and uranium are of particular importance in mining exploitation context, and carbonate uranium complexes - particularly [Formula see text] complexes - are crucial for environmental monitoring. The latter complexes are key species for the aqueous speciation of uranium, even in reducing environment where U(IV) low solubility usually governs uranium mobility. Fipronil (FP) is an emerging insecticide, however, its occurrence in drinking water and source water is limited. In this work, a total of 789 tap water and 95 ground water samples were collected from China in June 2019 in order to assess occurrence of FP and its derivatives (FPs). FPs were also analyzed in source, treated (n = 10, July), and tap water samples (n = 81, July and October 2019) originating from the central Yangtze River and its tributary, the Hanshui River in Wuhan. The sum concentrations of FPs (ΣFPs) in the tap water in China ranged from not detected (ND) to 5.07 (median 0.03 ng/L), with FP found in 55.3% of the samples, and other targets ≤ 50.0%. Significant regional variations in the ΣFPs values were found between East China (75th percentile 0.31 ng/L) and Northwest China (0.04), as well as between East China and North China (0.04). Similar ΣFPs values were found for ground water and tap water. The estimated daily intake of ΣFPs via water ingestion was below 200 pg/kg-bw/day for all age groups and was lower than the reference dose for FP (0.2 μg/kg-bw/day). Additionally, FPs were found in all of the source water samples collected in Wuhan with concentrations in the range of 0.84-2.72 ng/L for ΣFPs (median 2.39). Most of these FPs were removed during water treatment. Higher concentration of ΣFPs in tap water was observed in July (median 0.04 ng/L) compared to that in October (ND). This is the first study on the occurrence of FPs in the Yangtze River, the fate of FPs during the tap water treatment, and the regional distribution of FPs in tap water from China. In this study, the effects of excess nickel (Ni) (100 μM and 200 μM) on growth, antioxidant production, fatty acid, organic and amino acids profiles were examined in Lemna minor L. After 7 days of Ni treatment, chlorosis, growth inhibition and ROS overproduction were observed, accompanied by Ni accumulation. Interestingly, decreased malondialdehyde (MDA) levels were recorded in fronds upon Ni exposure. Fatty acid profiles in Ni-treated L. minor were characterized by increases in saturated- and decreases in unsaturated fatty acids. Ni excess increased the activities of antioxidant enzymes such as superoxide dismutase (SOD), guiacol peroxidase (GPX), and glutathione reductase (GR), and non-enzymatic antioxidants such as glutathione (GSH) and ascorbic acid (AsA); however, deactivation of ascorbate peroxidase (APX) and catalase (CAT) activities were also observed. Disruption of amino acid metabolism in Ni-exposed fronds was evidenced by the accumulation of cysteine, arginine, threonine, valine, isoleucine, leucine, lysine and phenylalanine, as well as reduced levels of tyrosine, alanine, aspartate and proline. Approximately 299%-396%, 139%-254% and 56%-97% concentration increments in citric, malic and oxalic acids, respectively, were concomitantly observed with significant decreases in tartaric, acetic, and fumaric acids in fronds subjected to Ni stress. Taken together, these results indicated that Ni stress induced negative effects on plant physiological, biochemical and morphological processes; however, it is likely that the coordination of metabolites and antioxidants may ameliorate the damaging effects of Ni accumulation. 1-Nitropyrene (1-NP) is a representative nitro-polycyclic aromatic hydrocarbon from diesel exhaust. Recently, we found that maternal 1-NP exposure caused fetal growth retardation and disturbed cognitive development in adolescent female offspring. To investigate long-term 1-NP exposure on spermatogenesis and steroidogenesis, male mice were exposed to 1-NP (1.0 mg/kg/day) by gavage for 70 days. There was no significant difference on relative testicular weight, number of testicular apoptotic cells and epididymal sperm count between 1-NP-exposed mice and controls. Although long-term 1-NP exposure did not influence number of Leydig cells, steroidogenic genes and enzymes, including STAR, P450scc, P45017α and 17β-HD, were downregulated in 1-NP-expoed mouse testes. Correspondingly, serum and testicular testosterone (T) levels were reduced in 1-NP-exposed mice. Additional experiment showed that testicular GRP78 mRNA and protein were upregulated by 1-NP. selleck products Testicular phospho-IRE1α and sliced xbp-1 mRNA, a downstream molecule of IRE1α, were elevated in 1-NP-exposed mice. Testicular phospho-PERK and phospho-eIF2α, a downstream molecule of PERK pathway, were increased in 1-NP-exposed mice. Testicular NOX4, a subunit of NAPDH oxidase, and HO-1, MDA, two oxidative stress markers, were increased in 1-NP-exposed mice. Testicular GSH and GSH/GSSG were decreased in 1-NP-exposed mice. These results suggest that long-term 1-NP exposure induces reactive oxygen species-evoked ER stress and disrupts steroidogenesis in mouse testes.