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In this study, the antioxidant ability of peanut shell and skin extracts and their effects on the physical and structure properties of starch-chitosan film were investigated. The results showed that the DPPH radical scavenging ability of peanut skin extracts was significantly higher than the peanut shell extracts. This could be due to the rich rutin and 4-O-caffeoulquinic acid existed in the peanut skin extracts. When added the peanut skin and shell extracts into the starch-chitosan film, the apparent viscosity of film forming solution at 100 s-1 decreased. Moreover, water vapor permeability and swelling of film decreased with the addition of peanut skin and shell extracts. Two peanut extracts also increased the color L* and opacity of film. The tensile strength of film increased with the addition of peanut skin extracts, and decreased with peanut shell extracts. The addition of two extracts also resulted in the increase of endothermic temperature of starch-chitosan film. But there were no new peaks appeared in the FTIR image. Only the peaks at 3276 cm-1, 1382 cm-1, 1249 cm-1 shifted to 3273 cm-1, 1385 cm-1 and 1258 cm-1, which implied the peanut shell and skin extracts disturbed the hydrogen bond and vibration of molecular chain in film matrix. V.Polylactic acid (PLA) is a biodegradable and biocompatible polyester derived from renewable resources like corn starch, presenting great potential in clinical applications like tissue engineering, implants and drug delivery systems. However, the intrinsic brittleness restricts its real applications. In this work, PLA nanocomposites were prepared by incorporating a small amount of carboxyl functionalized multi-walled carbon nanotubes (CNTs) and surface compatabilized montmorillonite (MMT) via technologies of freeze-drying and masterbatch-based melt blending. In the resulting nanocomposites, a well-distributed nano-filler network with microstructures of 1-D CNTs/2-D MMT platelets is formed favored by the enhanced interfacial interaction between the organic modified fillers with PLA matrix. Thanks to the well dispersed organic modified nanofillers, a large number of microcracks and extremely stretched PLA matrix are induced during tensile process, dissipating amounts of energy. As a result, the filler networks reinforce PLA with increment of 19% in modulus, remarkably increase by 13.8 times in toughness relative to PLA control without sacrificing strength. Thus, the PLA nanocomposites with excellent properties prepared through the facile and effective route possess broad prospect in biomedical applications. V.This study reports encapsulation-vitrification of Leydig cells. The Leydig cells were encapsulated in sodium alginate beads of different sizes and cryopreserved by vitrification or slow freezing. read more -chemical characterization of beads was done by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Fluorescence Recovery after Photobleaching (FRAP) and in vitro biodegradation study. Surface morphology of cryopreserved cell-encapsulated beads was evaluated by Environmental Scanning Electron Microscopy (E-SEM), encapsulation efficiency and viability of cells were assessed by Trypan blue assay, mitochondrial activity (MTT assay) and cytoplasmic esterase enzyme activity (FDA assay), respectively. Results showed that vitrification gives better results than slow freezing with respect to surface morphology as well as cell viability of the cell-encapsulated beads (86.94 ± 2.20% vs. 67.94 ± 2.30%; p  less then  0.05). Encapsulation of cells in small diameter beads (1.8 mm) gave a better cell proliferation rate than large (2.1 mm and 2.7 mm). There was a significant difference in the population doubling time (47.9 ± 1.7 h vs. 67.1 ± 2.5 h) and cell proliferation rate (0.50 ± 0.24 vs. 0.36 ± 0.24 per day) of vitrified-warmed cell encapsulated beads with different diameter (p  less then  0.05). Encapsualtion in sodium alginate beads is a promising method for cryopreservation of Leydig cells by slow freezing as well as vitrification. In this study, a pectin was extracted from Akebia trifoliata var. australis fruit peel waste using water solution, and its physicochemical properties were evaluated. The pectin was rich in galacturonic acid (GalA) content (76.68%). The degree of esterification (DE) and molecular weight (Mw) were 37.60% and 29,890 Da, respectively. The pectin structure was determined using Fourier transform-infrared (FT-IR) and Hydrogen nuclear magnetic resonance (H-NMR). The pectin exhibited an amorphous nature, negative charge, and good solubility. #link# The pectin was then used as a wall-material to coat curcumin-loaded zein nanoparticles for the first time. The obtained nanoparticles (curcumin-loaded core-shell nanoparticle, CLCSNs) exhibited a core (zein)-shell (pectin) structure and a spherical shape with an average diameter of 230 nm. The electrostatic attraction, hydrogen bonding, and intermolecular interaction were involved in the CLCSNs formation. A high encapsulation efficiency (EE, 89.65%) and loading capacity (LC, 10.35%) of the CLCSNs were obtained for the curcumin. The solubility, stability, antioxidant activity, and in vitro bioavailability of the curcumin were significantly increased after loading into the CLCSNs. Therefore, this sustainable pectin from Akebia trifoliata var. australis fruit peel waste represents a promising natural macromolecule for use in the pharmaceutical and food industries. Bacteroides thetaiotaomicron (B. thetaiotaomicron), which resides in the human intestinal tract, has a number of carbohydrate enzymes, including glycoside hydrolase (GH) family 97. Only a few GH 97 enzymes have been characterized to date. In this study, a novel α-galactosidase (Bt_3294) was cloned from B. thetaiotaomicron, expressed in Escherichia coli, and purified using affinity chromatography. This novel enzyme showed optimal activity at 60 °C and pH 7.0. Enzyme activity was reduced by 94.4% and 95.7% in the presence of 5 mM Ca2+ and Fe2+, respectively. It is interesting that Bt_3294 specifically hydrolyzed shorter α-galactosyl oligosaccharides, such as melibiose and raffinose. The D-values of Bt_3294 at 40 °C and 50 °C were about 107 and 6 min, respectively. After immobilization of Bt_3294, the D-values at 40 °C and 50 °C were about 37.6 and 29.7 times higher than those of the free enzyme, respectively. As a practical application, the immobilized Bt_3294 was used to hydrolyze raffinose family oligosaccharides (RFOs) in soy milk, decreasing the RFOs by 98.