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This study aimed to find a new source of anti-leukemia agents from Vietnamese medicinal plants.

The human leukemia cell lines TCCY, KU-812, TCC-S, KOPB-26, and HL60 were used. The crude ethanol extracts of 17 medicinal plants were collected and evaluated for their cytotoxicity against these leukemia cell lines by the trypan blue dye exclusion test. Morphological changes of cells were observed under phase-contrast inverted microscope. Bioactive compounds were evaluated using the method described by Ciulei. 2,2-diphenyl-1-picrylhydrazyl (DPPH) method was carried out for evaluating the antioxidant effect.

Among the tested samples, Artemisia vulgaris (A.vulgaris) crude ethanol extract effectively inhibited the viability of leukemia cell in both dose and time-dependent manner. The IC50 value was different for cell lines and ranged from 18.07±1.64 µg/ml to 45.87±3.49 µg/ml. Moreover, the phytoconstituents analysis results showed coumarin, flavonoid, anthocyanin, cardiac glycoside, tannins, reduced sugar compounds were present in the A.vulgaris extract. The total polyphenol and flavonoid contents of the dry extract were calculated as 3.81 mg GAE/g dry weight and 11.64 mg RUE/g dry weight of A.vulgaris. A.vulgaris exhibited antioxidant activity with IC50 is 145.10 ± 6.34 µg/ml.

Among the 17 Vietnamese plants used to treat a variety of cancer-related diseases, A.vulgaris has been able to suppress the growth of leukemia cells.

Among the 17 Vietnamese plants used to treat a variety of cancer-related diseases, A.vulgaris has been able to suppress the growth of leukemia cells.

This study was designed to explore the effect of miR-122a on the biological behavior of laryngeal carcinoma cells and its role.

Thirty-two patients with laryngeal carcinoma who were diagnosed and treated in our hospital from March 2013 to November 2015 formed the research group (RG), and 30 normal people who underwent physical examination in our hospital during the same period formed the control group (CG). We observed the expression of miR-122a in cells and its effect on cell biological function, examined the expression level of miR-122a in laryngeal carcinoma tissues, and further drew receiver operating characteristics (ROC) curve to analyze the diagnostic value of miR-122a in laryngeal carcinoma; we divided them into high and low expression groups according to the expression of miR-122a, and also registered their 3-year survival rate.

miR-122a showed low expression in cancer tissues (p<0.05). ROC curve analysis revealed that miR-122a had a sensitivity of 82.22%, specificity of 68.75%, and area under the curve (AUC) of 0.770. The 3-year survival rate of the high expression group was 56.25%, and that of low expression group was 25.00%. The survival rate of high expression group was significantly better than that of low expression group (p=0.024). The proliferation ability of AMC-HN-8 cells transfected with miR-122a-mimics sequence was obviously inhibited, and its apoptosis rate increased.

Upregulation of miR-122a expression can reduce proliferation of laryngeal carcinoma cells and increase their apoptosis, and it can be used as a potential diagnostic index and therapeutic target for laryngeal carcinoma.

Upregulation of miR-122a expression can reduce proliferation of laryngeal carcinoma cells and increase their apoptosis, and it can be used as a potential diagnostic index and therapeutic target for laryngeal carcinoma.

To explore the efficacy of nedaplatin combined with docetaxel in patients with nasopharyngeal carcinoma and its influence on the expressions of esophageal cancer-related gene 4 (ECRG4) and vascular endothelial growth factor (VEGF).

86 patients with nasopharyngeal carcinoma, admitted to and treated in our hospital from March 2016 to February 2018, were selected and randomly divided into control group (n=43) and observation group (n=43). Chemotherapy combining cisplatin with fluorouracil was administered in the control group, while nedaplatin combined with docetaxel was given in the observation group. Then the efficacy, adverse reactions, the levels of serum hypoxia-inducible factor-1α (HIF-1α) and VEGF in patients before and after treatment, and the change in the expression level of ECRG4 in foci after treatment were compared between the two groups. After 1-year follow-up, the improvement in quality of life was compared between the two groups of patients.

The objective remission rate and disease control h milder adverse reactions, and it can reduce the levels of serum HIF-1α and VEGF, and up-regulate ECRG4 expression in patients, exerting an anti-carcinoma effect.

To observe the postoperative efficacy of low-temperature plasma radiofrequency ablation in treating elderly patients with laryngeal carcinoma and its influence on the tumor markers, cyclooxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) expressions in laryngeal carcinoma tissues.

A total of 50 elderly laryngeal carcinoma patients undergoing low-temperature plasma radiofrequency ablation in our hospital from January 2015 to January 2018 were enrolled as observation group, and another 50 elderly laryngeal carcinoma patients receiving conventional surgical treatment as control group. At the end of the treatment course, the efficacy and postoperative complications in the patients were observed, the operation time and pain visual analogue scale (VAS) and mucosal recovery scores were recorded. Besides, the levels of the tumor markers carbohydrate antigen 125 (CA125), CA199 and carcinoembryonic antigen (CEA) were determined, and the expressions of COX-2 and VEGF in laryngeal carcinoma tissues in l practice.

Low-temperature plasma radiofrequency ablation has better efficacy in treating elderly laryngeal carcinoma patients, with few postoperative complications, and decreased expression levels of postoperative tumor markers as well as COX-2 and VEGF in laryngeal carcinoma tissues, and it offers better overall effect and notable application value, therefore it is worth trying it in clinical practice.

Pancreatic cancer is a fatal malignant tumor with no obvious characteristics in the early stage of onset and high metastatic ability which results in a low survival. find more Understanding the detailed process of pancreatic cancer contributes to new treatments to prolong patients' survival.

We carried out an in-depth analysis by modularization while seeking for critical genes in the pathogenesis of pancreatic cancer so as to identify the molecular mechanisms of the condition using differential analysis, co-expression module analysis, enrichment analysis, and network connectivity analysis. In light of the hypergeometric test, ncRNA (non-coding RNA) and transcription factors that regulate the module would be predicted.

Conclusively, seven co-expression modules were obtained, in which CPA2 and A1BG were significantly differentially expressed in patients who had pancreatic cancer with active regulation in dysfunction modules. The modular genes significantly participated in second-messenger-mediated signaling as well as cellular calcium homeostasis and also controlled the interactions of neuroactive ligand-receptor.