Heideballe0978

Collectively, this study has linked the PTEN/AKT signaling axis to deregulated epigenetic changes in myeloid malignancies. It also provides a rationale for mechanism-based therapy for patients with ASXL1 mutations. © The Author(s) 2020. Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS.Colletotrichum species are the most important postharvest spoilage of papaya fruit. The objective of this research was to evaluate the effect of temperature and relative humidity on growth rate and time for growth to become visible of five strains of Colletotrichum gloeosporioides isolated from papaya fruit in a complex medium. this website As a primary model, the radial growth rates were estimated using the Baranyi and Roberts model in papaya agar. The Solver Excel function was used to obtain the time for visible mycelium (tv). Secondary models obtained with the Rosso's cardinal model with inflection were applied to describe the effect of temperature on radial growth rate (µ, mm/d). The Arrhenius-Davey model was used to model tv. The obtained models seem to be satisfactory for describing the µ and time tv. The relative humidity (RH) had influence on µ and time tv for all tested C. gloeosporioides isolates, but no model is able to describe the behavior of the fungus. External validation of models on papaya fruit was performed. Growth models were developed with the same models used in vitro. The bias (Bf) and the accuracy factors (Af), as indices for performance evaluation of predictive models in food microbiology, as a function of temperature and RH, were 1.22 and 1.33 for growth rate, and 1.18 and 1.62 for time for visible mycelium respectively indicating good predictions. The supply chain of papaya is complex and require constant conditions and mismatches in can cause damage to fruit. Knowledge of the behavior of C. gloeosporioides on papaya fruit and application of the developed models at the supply chain level will help to establish transport control strategies against these molds. This research has contributed to the first models of growth for C. gloeosporioides in Mexico.OBJECTIVES Patients with complex congenital heart disease carry an increased risk of damage to retrosternal structures each time they undergo redo sternotomy. The aim of this study was to evaluate the safety and efficacy of neck cannulation for peripheral cardiopulmonary bypass to alleviate the risks in high-risk redo sternotomy patients. METHODS Children and adults with congenital heart disease undergoing high-risk redo sternotomy were included in this retrospective study. The primary outcome was the safety and efficacy of neck cannulation for cardiopulmonary bypass. The secondary outcome was to assess preoperative risk factors as an indication for neck cannulation. The right common carotid artery and right internal jugular vein were cannulated and full cardiopulmonary bypass was initiated with vacuum-assisted venous drainage. Redo sternotomy was performed on a decompressed heart, and bifrontal regional cerebral oxygen saturation was monitored via near-infrared spectroscopy. RESULTS In total, 35 patients were included. No mortality, neurological or vascular complications occurred postoperatively. Mean left- and right-sided near-infrared spectroscopy were 70.0% (±10.5) and 64.2% (±12.0), respectively, and the mean difference was 5.7% (±6.9). Main preoperative risk factors were; adherent ascending aorta (45.7%), adherent conduit (40%), severely dilated retrosternal right ventricle (17.1%) and skeletal deformations (14.3%). CONCLUSIONS Cannulation of the right neck vessels for peripheral cardiopulmonary bypass prior to high-risk redo sternotomy in children and adults with congenital heart disease is a safe and effective strategy. In combination with near-infrared spectroscopy monitoring, adequate cerebral oxygenation can be ensured while the risk of catastrophic haemorrhage is minimized. © The Author(s) 2020. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.A best evidence topic in cardiac surgery was written according to a structured protocol. The question addressed was 'Is it safe and effective to reduce the target anticoagulation range for patients with mechanical aortic valves?' Altogether 922 papers were found using the reported search, of which 7 represented the best evidence to answer the clinical question. Only studies that compared high (target international normalized ratio 2-3) versus low (target international normalized ratio less then 2-3) intensity anticoagulation were included. The authors, journal, date and country of publication, patient group studied, study type, relevant outcomes and results of these papers are tabulated. We conclude that there is growing evidence for the reduction of the target anticoagulation range for patients with mechanical prosthetic aortic valves, especially bileaflet valves with presumed better haemodynamic properties. Several large randomized controlled trials and a meta-analysis have concluded that reducing the target international normalized ratio range (below the conventional range of 2-3) for mechanical aortic valves in patients with no thrombogenic risk factors produces less bleeding and does not increase thromboembolic events. © The Author(s) 2020. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.OBJECTIVES The interstage mortality rate after a Norwood stage 1 operation remains 12-20% in current series. In-hospital interstage facilitates escalation of care, possibly improving outcome. METHODS A retrospective study was designed for hypoplastic left heart syndrome (HLHS) and HLHS variants, offering an in-hospital stay after the Norwood operation until the completion of stage 2. Daily and weekly examinations were conducted systematically, including two-dimensional and speckle-tracking echocardiography. Primary end points included aggregate survival until the completion of stage 2 and interstage freedom from escalation of care. Moreover, we calculated the sensitivity and specificity of speckle-tracking echocardiographic myocardial deformation in predicting death/transplant after the Norwood procedure. RESULTS Between 2015 and 2019, 33 neonates with HLHS (24) or HLHS variants (9) underwent Norwood stage 1 (31) or hybrid palliation followed by a comprehensive stage 2 operation (2). Stage 1 Norwood-Sano was preferred in 18 (54.