Hancockstraarup6207

This sustainable microbial biopolymer shows the possibility to be used as a bioink for 3D bioprinting.Grape pomace (GP) is a major by-product from the wine industry, known for its bioactive compounds and their impact upon gastrointestinal (GI) health. However, bioaccessibility is often poor due to their degradation during digestion. This work aimed to encapsulate bioactive GP extract (GPE) into chitosan (CS) and alginate (Alg) nanoparticles (NPs) to mitigate degradation in the GI tract. Alg and CS NPs were optimized using a rotatable central composite design and NPs were characterized for their size, polydispersity, zeta potential and total phenolics (TP) association efficiency. The best formulations showed sizes ranging 523-853 nm, polydispersity indexes of 0.11-0.36, zeta potential of -15.0-14.9 mV and TP association efficiencies of 68 and 65%. FTIR confirmed that there was no formation of new chemical groups after association of the polymers with GPE. Both formulations improved the bioaccessibility of different phenolics following in vitro GI digestion, leading to increased antioxidant and antimicrobial activities. Moreover, the permeability of bioactive compounds through a Caco-2/HT29-MTX co-culture was reduced, suggesting a higher residence time in the intestine. Cy5.5 was used for tracking the CS NPs, which did not affect the metabolic activity of Caco-2 and HT29-MTX cells. selleck chemical Confocal microscopy images confirmed the adsorption of NPs to the cellular layer and suggested a reduction of the tight junction protein occludin when cells were incubated with Cy5.5-CS in solution. This study suggests that encapsulation of GPE can offer protection against along the GI tract and improve its biological activity with significant impact for oral delivery applications, including functional foods.Recent advances in regenerative medicine have given hope in overcoming and rehabilitating complex medical conditions. In this regard, the biopolymer poly-ε-caprolactone (PCL) may be a promising candidate for tissue regeneration, despite lacking the essential bioactivity. The present study used PCL nanofibers (NFs) scaffold decorated with the extracellular matrix proteins fibronectin and laminin combined for neuronal regeneration. The potential for the dual proteins to support neuronal cells and promote axonal growth was investigated. Two NFs scaffolds were produced with PLC concentrations of 12% or 15%. Under scanning electron microscopy, both scaffolds evidenced uniform diameter distribution in the range of 358 nm and 887 nm, respectively, with >80% porosity. The Brunauer-Emmett-Teller (BET) test confirmed that the fabricated NFs mats had a high surface area, especially for the 12% NFs with 652 m2/g compared to 254 m2/g for the 15% NFs. The proteins of interest were successfully conjugated to the 12% PCL scaffold through chemical carbodiimide reaction as confirmed by Fourier-transform infrared spectroscopy. The addition of fibronectin and laminin together was shown to be the most favorable for cellular attachment and elongation of neuroblastoma SH-SY5Y cells compared to other formulations. Light microscopy revealed longer neurite outgrowth, higher cellular projected area, and lower shape index for the cells cultured on the combined proteins conjugated fibers, indicating enhanced cellular spread on the scaffold. This preliminary study suggests that PCL nanoscaffolding conjugated with matrix proteins can support neuronal cell viability and neurite growth.Polydopamine nanoparticles (PDA NPs), among nature-inspired building materials, show special functions for biomedical systems and exploring PDA derived nanostructures for future developments is a fast growing field. Herein, we demonstrated the first evaluation of the PDA NPs for the electrochemical determination of lung cancer cells. In the presented study, PDA NPs were synthesized in a mild and cost-effective fashion by self-polymerization of dopamine in an alkaline environment. The structural and chemical characterizations clearly demonstrated the formation of PDA NPs with controllable size (130 nm), hence applied as a suitable material to functionalize the pencil graphite electrode (PGE) surface to construct a cytosensing nanoprobe. The ability of the developed sensor (PDA NPs/PGE) for label-free electrochemical A-549 lung cancer cells detection was investigated. The designed PDA NPs based cytosensor exhibited good biocompatibility and sensitivity for impedimetric diagnosis of A-549 cells in a wide linear range (1.0 × 102-1.0 × 105 cells mL-1) with low detection limit (25 cells mL-1). Furthermore, the developed bioassay has great potential as liquid biopsy for early cancer detection.The proposed study aimed to develop topical hydrogel containing ketoconazole loaded cubosomes with lower surfactant concentrations using the 'Quality by Design' (QbD) approach. Risk assessment was performed, followed by screening and optimization of formulations by 32 factorial design using Design-Expert® software. Keeping the combination of constituents similar to that of the optimized batches as predicted post conduct of 'Design of Experiment' (DoE) studies, scale-up batches were prepared. The 32 factorial design model successfully predicted the composition of the optimized formulation within the confidence limits. In vitro drug release study was performed and analyzed by various mathematical models. Ex vivo permeation study was investigated using goat ear skin. These ketoconazole loaded cubosomes showed a release pattern similar to the Korsmeyer-Peppas model experiencing Fickian diffusion having 67% cumulative ketoconazole release within 24 h. Ex vivo permeation study of hydrogel containing ketoconazole loaded cubosomes revealed a sustained release pattern through the goat ear skin with around 92.73 % release within 24 h. Scale-up studies also gave the confirmatory results for the post characterization studies, whereby the particle size of ketoconazole loaded cubosomes was 198 nm with 45% ketoconazole entrapment efficiency. This hydrogel containing ketoconazole loaded cubosomes can be used for topical drug delivery.