Gunnboone0996
The lipolytic yeast Yarrowia lipolytica produces cell-wall-associated lipases, namely Lip7p and Lip8p, that could have interesting properties as catalyst either in free (released lipase fraction-RLF) or cell-associated (cell-bound lipase fraction-CBLF) forms. Herein, a mixture of waste soybean frying oil, yeast extract and bactopeptone was found to favor the enzyme production. Best parameters for lipase activation and release from the cell wall by means of acoustic wave treatment were defined as 26 W/cm2 for 1 min for CBLF and 52 W/cm2 for 2 min for RLF. Optimal pH and temperature values for lipase activity together with storage conditions were similar for both the free enzyme and cell-associated one pH 7.0; T = 37 °C; and > 70% residual activity for 60 days at 4, - 4 °C and for 15 days at 30 °C.
The purpose of the study was to investigate the prevalence of poor health behaviors (low dietary quality, low physical activity (PA), and high body mass index (BMI)) in cancer patients and the general population and its relationship with receipt of patient-physician recommendations.
We analyzed data from the National Health and Nutrition Examination Survey (NHANES) 2005-2014 to compare 1846 patients with a history of cancer to 16,641 with no cancer history. BMI was measured during physical exam. Dietary quality and PA were obtained from a questionnaire, along with receipt of physician recommendations for each behavior.
Cancer patients had dietary quality that "needs improvement," were not meeting PA recommendations, and were overweight. Compared to the general population, dietary quality (54 vs. 54, p = .80), prevalence of physical inactivity (34% vs. 31%, p = .01), and BMI (28 vs. click here 28, p < .01) were similar. Among cancer patients, prevalence of physician recommendations to improve dietary quality (33n health behavior recommendations, especially in cancer patients, to improve patient outcomes.The prediction of dispersion of gases emitted from rooftop stacks in a built environment is important for preventing or minimizing their harmful effects on human health. In this study, the wind flow and dispersion of exhaust gas emitted from rooftop stacks on buildings in an urban environment under different atmospheric thermal stabilities were investigated using numerical simulations. The wind flow field and dispersion contaminants were simulated using a computational fluid dynamics model with the k-ε turbulent schemes being resolved by the Reynolds-averaged Navier-Stokes approach. An isolated building was modeled under conditions of varying thermal stratification of the boundary layers (neutral, unstable, and stable conditions). The diffusion flow field within the building wake zone was investigated for various stack sites (center, right side, and left side). Experiments were conducted in a wind tunnel to validate the numerical simulation results, by using the data qualitatively and quantitatively. The numerical simulation results were consistent with the experimental observations. The results indicated that the pollutant concentration of the plume spread was high near the stack and decreased with increasing distance from the stack. Under stable conditions, the flow motion and separation increased in the wake zone, and the pollutant concentration of the lateral spread at the average human height decreased. Under unstable conditions, the flow of the vortex circulation was fast and strong, and the pollutant concentration of the vertical spread was high.Hybrid aerogels are promising candidates for energy storage, biosensing, and medical applications, but the conventional fabrication methods, being time-consuming and complex, limit their widespread utilization. The critical issues affecting their functionality include the un-controllable particle dispersity, loading of active materials, and the porosity. We report a simple and efficient method to synthesize in situ reduced Au nanoparticles@graphene (Au@graphene) hybrid aerogel using near-infrared radiation (NIR), resulting the uniform loading of well-dispersed Au nanoparticles (Au-NPs) as well as in situ reduction of graphene oxide (GO) with enhanced conductivity. The concentration of iso-propylacrylamide and GO can be adjusted to control the aerogel pore size during the freeze-drying process. Reduction of HAuCl4 and GO to high extent under NIR light was confirmed with advanced characterization techniques. Density functional theory based calculations with generalized gradient-corrected functional (GGA/PW91) in the hybrid aerogel system, and dnd basis sets are used for the confirmation of possible interactions between the GO, Au-NPs, and the polymer. The as-designed highly porous and conductive aerogel shows an excellent humidity response (30-97%) and successfully removes the methylene blue pollutant from the aqueous solution to a high extent (90%). Therefore, Au@graphene hybrid aerogel is potentially an exciting candidate for a wide range of applications in the humidity sensing and biomedical disease detection.In the current study, we deal with the crucial role of 5'-deoxyadenosyl radical and water in the mechanism of the conversion of L-lysine into L-β-lysine. The DFT (density functional theory)-B3LYP method coupled with 6-31G(d) basis set has been performed to investigate the optimized structures of transition states (TSs) and intermediates (IMs) of two processes in water (i) the attack of 5'-deoxyadenosyl radical to complex PLP-L-lysine and (ii) hydrolysis to liberate L-β-lysine. Meanwhile, M062X/6-311++g(3df,2p) level of theory is applied to compute the relative Gibbs energy ΔG. Procedure (i) has undergone various steps but includes two main structural aziridinyl rings TS2 (ΔG = 4.1 kcal/mol) and TS3 (ΔG = 2.3 kcal/mol). In stage (ii), hydroxy group of water would help to break the bond between β-NH2 group of L-lysine and PLP better than that of Tyr389.
Camelina biotypes had different responses to freezing stress, which was mainly inherited by additive gene effects and can be reliably used in breeding programs and for a better understanding of freezing tolerance mechanisms in camelina plants. Camelina [Camelina sativa (L.) Crantz] is a frost-tolerant oilseed plant that is cultivated as an autumn crop in semi-arid regions. However, camelina establishment in these areas is limited by low temperatures in winter that results in decreased seed yield. In the present study, genetic basis of freezing tolerance (FT) in spring and winter biotypes of camelina was analyzed at seedling stage using a diallel cross experiment. The parents consisted of two winter doubled haploid (DH) lines with high (DH34 and DH31), two spring lines with medium (DH19 and DH26), and two spring lines with low FT (DH08 and DH91). For this purpose, the parents along with F
entries were subjected to freezing stress and survival percentage, electrolyte leakage, and lethal temperature for 50% mortality (LT
) of the lines were measured.
