Gonzalezbusk1587

Recent studies indicated that 'inert ingredients' exert negative effects on the environment. Puromycin clinical trial Herbicide safeners are classed as 'inert ingredients', which increase the selectivity and detoxification of herbicides. However, little attention has been focused on the environmental behavior of herbicide safeners. AD-67 (AD), fenclorim (FM), and flurazole (FZ) are three commercially available herbicide safeners. In this study, zebrafish embryos were used as a model to investigate the potential developmental toxicity of these three safeners. The results showed that AD, FM, and FZ 96 h-LC50 values were 2.52, 1.26, and 2.01 mg/L, respectively. Significant decreased body lengths were observed in embryos after 96 h of exposure to 0.2 mg/L FM and FZ. Hatching rates significantly increased at 24 h and decreased at 96 h in all treatment groups (0.02 mg/L AD, 0.2 mg/L AD, 0.02 mg/L AD, 0.2 mg/L FZ, as well as 0.01 and 0.1 mg/L FM). No significant (p > 0.05) changes in heartbeat numbers (60 s), but clearly increased malformation rates were observed in response to safeners exposure. Furthermore, embryos showed signs of oxidative stress, such as decreased activities of superoxide dismutase, altered activities of glutathione reductase and catalase and cell apoptosis. The gene transcription related to body malformation (egf, krt 17, and tbx 16) and cell apoptosis (bcl 2 and bax) changed in treated groups. These genes have been connected to potential toxicological mechanisms. These results indicate that the herbicide safeners AD, FM, and FZ induced developmental toxicities in zebrafish embryos. This study is the first report of the toxicity of safeners in the development of zebrafish embryos. The results may be useful for assessing the risk of herbicides safeners in the aquatic ecosystem.In this work, green tea extracts synthesized nanoscale zero-valent iron/nickel (GT-nZVI/Ni) was prepared and the Cr(VI) contaminated soil column was remediated by GT-nZVI/Ni suspension. The influence factors including the concentration, pH value and flow rate of GT-nZVI/Ni suspension were studied. Under the conditions of pH = 4, concentration of 0.15 g/L and flow rate of 1.25 mL/h, GT-nZVI/Ni suspension had the best reduction and immobilization effect on Cr(VI) in the soil column. Na+ and Ca2+ can promote the immobilization of Cr (VI) in soil, while humic acid weakened the immobilization of Cr (VI). After GT-nZVI/Ni is injected into the soil column, the content of weak acid extractable and reduced chromium is significantly reduced, and the toxic hazard of hexavalent chromium in the soil is greatly reduced. The 1D-CDE model was used to fit the breakthrough curves of Fe(tot), Fe(aq) and Fe(0), and the migration of GT-nZVI/Ni in Cr(VI) contaminated soil was simulated and predicted. Compared with the inert solute Cl-, the breakthrough curves of Fe (tot), Fe (aq) and Fe (0) in Cr (VI) contaminated soil column were significantly lagged, with delay coefficients of 2.465, 2.322 and 3.288, respectively. The reaction of GT-nZVI/Ni with Cr (VI) led to the decrease of Fe mobility. Finally, the outflow concentration of Fe (tot) was 0.064 g/L, and the loss was mainly due to reaction and retention in the soil. About 57.89% of GT-nZVI/Ni was retained in the soil.Marine microalgae with high removal efficiency of phenol are needed for the remediation of polluted seawater in cases involving phenol spills. To achieve this purpose, adaptive laboratory evolution (ALE) was performed by a microalga Isochrysis galbana Parke MACC/H59, which is capable of degrading phenol at concentrations of less than 100 mg L-1 in 4 d. Two acclimation conditions were used (i) 90 d at 100 mg L-1 phenol, and (ii) 90 d at 100 mg L-1 phenol followed by another 90 d at 200 mg L-1 phenol. By doing so, two strains (PAS-1 and PAS-2) could be obtained respectively. They grew rapidly at phenol concentrations up to 200 mg L-1 and 300 mg L-1, respectively, with a specific growth rate 2.52-3.40 times and 1.93-3.23 times that of the control (without phenol). Also, both strains had a higher removal capacity of phenol than the unacclimated alga. Phenol at an initial concentration of 200 mg L-1 was completely removed in 5 d thereby. For 300 mg L-1 phenol, a removal efficiency of 92% was achieved in 10 days by using PAS-2, with a removal rate constant of 30.01 d-1 (about twice that of PAS-1) and a half-life of 4.90 d (about half that of PAS-1), showing that a better strain may be obtained by extending the acclimation time. The enhancement of phenol biodegradation can be explained by the elevated activity of phenol hydroxylase (PH) in both strains. These results indicated that ALE could be an efficient tool used to enhance the tolerance and biodegradation of marine microalgae to phenol in seawater.Biochar plays an essential role in soil remediation, but its effect on the arsenic remediation has been controversial. In this study, hexadecyl trimethyl ammonium bromide (HDTMA-Br) modified or unmodified biochar on As mobility and bioavailability in soil were studied. The sequential extraction experiment showed that As in the original soil mainly existed in the occluded form (78.24%), followed by Fe‒As (20.72%) and Al‒As (0.88%) forms. With the addition of the modified and unmodified biochars, the contents of Ca‒As and Fe‒As increased by 0.36 - 0.95% and 2.06 - 3.36%, respectively, suggesting the increased potential toxicity of As. The NaH2PO4 extraction result showed that the unmodified biochar increased the As availability by 3.23 - 22.76%, whereas the HDTMA-modified biochar reduced the As availability by 4.80 - 13.41%. Pot experiment showed that the unmodified and modified biochar increased the biomass of Brassica pekinensis, and the modified biochar (HB5) decreased the uptake of As by plants by 80.77% compared to the unmodified biochar. In particular, the plant achieved better growth in the modified biochar treatment (average height 8.31 cm) than in the unmodified biochar treatment (average height 6.97 cm). Therefore, both biochars facilitated phase transformation of As from the stable to the mobile states in the soil. Nevertheless, the HDTMA-modified biochar had an effect on alleviating As bioavailability and toxicity.