Goldenhigh1286
The same column geometry was tested for the purification of C-phycocyanin from clarified as well as cell-laden Arthrospira platensis feedstocks. This represents the first demonstration of one-step printed stationary phases to capture proteins directly from solid-laden feedstocks. We believe that the material presented here represents a significant improvement towards implementation of three-dimensional printed chromatography media in the field of separation science.
Clinical assessment of uterine artery (UtA) hemodynamics is currently limited to ultrasound (US) Doppler velocimetry. We previously demonstrated the feasibility of 4D flow magnetic resonance imaging (MRI), which allows flow quantification throughout the entire vessel, to evaluate UtA hemodynamics during pregnancy. In this study, we seek to further validate the physiologic relevance of 4D flow MRI measures of UtA blood flow rate by exploring their associations with pregnancy outcomes relative to US-based metrics.
This was a cross-sectional study of 87 singleton pregnant women, enrolled between May 2016 and April 2019, who underwent a 4D flow MRI scan to measure UtA pulsatility index (PI) and blood flow rate (in mL/min). UtA PI was also measured using US. The primary outcome was a composite (COMP) of either preeclampsia (PEC), small for gestational age (SGA), or both PEC+SGA. The secondary outcomes were PEC and SGA individually. Wilcoxon rank-sum tests were performed to study the ability of MRI-flow, MRI-PI=0.04). The AUCs for MRI-flow, MRI-PI, and US-PI in predicting COMP were comparable (0.69, 0.74, and 0.73) with non-significant differences among the three AUCs (p=0.87).
4D flow MRI can yield physiologic measures of UtA blood flow rate and PI that are associated with adverse pregnancy outcomes. This may open new avenues in the future to explore more robust MRI-based evaluation of UtA hemodynamics in pregnancy. This article is protected by copyright. All rights reserved.
4D flow MRI can yield physiologic measures of UtA blood flow rate and PI that are associated with adverse pregnancy outcomes. This may open new avenues in the future to explore more robust MRI-based evaluation of UtA hemodynamics in pregnancy. This article is protected by copyright. All rights reserved.
Pasta is a staple food that is consumed worldwide and is an excellent product for the addition of ingredients rich in bioactive compounds. The fortification of pasta with such compounds could represent a healthy choice for consumers.
In this study, fresh pasta was formulated by replacing durum wheat semolina with 0, 5, 10, and 15 g 100 g
of dried Moringa oleifera leaf powder (MOLP), rich in fibers, minerals, and antioxidant compounds. Increasing levels of MOLP influenced the technological and nutritional properties of wheat-based fresh pasta. Moringa oleifera reduced the optimum cooking time, the swelling index and firmness, while increasing the cooking loss and adhesiveness. From a nutritional viewpoint, the inclusion of MOLP enhanced the phenol content, the antioxidant activity, and the mineral content of fresh pasta. The products obtained had good sensorial acceptability and can make several nutritional claims due to MOLP richness minerals.
The fortification of fresh pasta with MOLP could represent a valuable strategy to increase the nutritional value of the product, preserving pasta technological properties without affecting sensory acceptability. © 2020 Society of Chemical Industry.
The fortification of fresh pasta with MOLP could represent a valuable strategy to increase the nutritional value of the product, preserving pasta technological properties without affecting sensory acceptability. © 2020 Society of Chemical Industry.Due to the shot-to-shot variability in tool mark reproduction on fired cartridge cases, a method of replication is needed for the creation of training and testing sets. Double-casting is one method that has been used for this application, but the accuracy and variability of this method needs to be characterized. Three firearms were used to fire 25 cartridges each to create the master cartridge cases. The double-casting method consists of creating a silicone mold of the master cartridge case. A plastic resin mix is then poured into the mold to create the double-cast reproduction. Fifteen double-casts of each of the 75 fired cartridge cases were created across different silicone molds to analyze within- and between-mold variability. The master cartridge cases and double-casts were scanned with a confocal microscope (Sensofar® S neox) to create three-dimensional representations of the surfaces. Two similarity metrics were used for the objective comparison of the double-casts to their master cartridge cases the areal correlation coefficient (ACCFMAX ) and the number of congruent matching cells (CMC). The ACCFMAX and CMC data, along with visual examinations, showed that the double-casting method produces accurate reproductions. Within-mold variability was found to be minimal, and between-mold variability was low. These results illustrate that double-casting can be applied for training and testing purposes.In order to assess the extent of agreement between forensic footwear examiners in the United States, a reliability study was performed by West Virginia University between February 2017 and August 2018. Over the span of 19 months, 70 examiners each performed 12 comparisons and reported a total of 840 conclusions. For each comparison, participants were queried on a number of factors in order to determine the degree to which different types of features were identified, evaluated, and weighted, before arriving at a final decision regarding the strength of the association or disassociation between questioned and test impressions. Preliminary results from this study are divided into a series of three summaries. This manuscript (Part I) describes participant demographics as well as community agreement in both feature identification/annotation, and final reporting. Results indicate considerable variation in feature identification/annotation (as low as 66.5% agreement), but higher consistency in the reporting of overall conclusions. For mated pairs, this agreement was 79.7% ± 14.1% (median of 85.7% and a 90% confidence interval between 75.9% and 83.2%). For nonmated pairs, the equivalent overall agreement was 89.8% ± 6.69% (median of 91.4% and a 90% confidence interval between 87.4% and 92.1%). These estimates of agreement are further compared with previous published findings, and collectively, the work extends the body of knowledge concerning reliability in forensic footwear comparisons and conclusions.
In in vitro fertilisation (IVF) with or without intracytoplasmic sperm injection (ICSI), selection of the most competent embryo(s) for transfer is based on morphological criteria. However, many women do not achieve a pregnancy even after 'good quality' embryo transfer. One of the presumed causes is that such morphologically normal embryos have an abnormal number of chromosomes (aneuploidies). Preimplantation genetic testing for aneuploidies (PGT-A), formerly known as preimplantation genetic screening (PGS), was therefore developed as an alternative method to select embryos for transfer in IVF. In PGT-A, the polar body or one or a few cells of the embryo are obtained by biopsy and tested. Only polar bodies and embryos that show a normal number of chromosomes are transferred. The first generation of PGT-A, using cleavage-stage biopsy and fluorescence in situ hybridisation (FISH) for the genetic analysis, was demonstrated to be ineffective in improving live birth rates. #link# Since then, new PGT-A methodologies havevidence of a difference in cumulative live birth rate, live birth rate after the first embryo transfer, or miscarriage rate between IVF with and IVF without PGT-A as currently performed. No data were available on ongoing pregnancy rates. The effect of PGT-A on clinical pregnancy rate is uncertain. Women need to be aware that it is uncertain whether PGT-A with the use of genome-wide analyses is an effective addition to IVF, especially in view of the invasiveness and costs involved in PGT-A. PGT-A using FISH for the genetic analysis is probably harmful. link2 The currently available evidence is insufficient to support PGT-A in routine clinical practice.
Soybean meal (SBM) is the most common protein source used in the poultry and livestock industries. It has high-quality protein, an excellent amino acid (AA) profile, and positive isoflavone properties. However, the antigen proteins in SBM are unsuitable for young animals. link3 The objective of this study was to identify a Bacillus strain that can degrade soybean antigen proteins, and to evaluate the feasibility of its application in SBM fermentation.
Bacillus velezensis DP-2 was isolated from Douchi, a fermented Chinese food. It degraded 96.14% and 66.51% of glycinin and β-conglycinin, and increased the trichloroacetic acid-soluble protein (TCAN) content by 5.46 times in the SBM medium. DP-2 could secrete alkaline protease and neutral protease, with productivities of 5.85 and 5.99 U mL
. It had broad-spectrum, antibacterial activities against Rhizopus nigricans HR, Fusarium oxysporum ACCC37404, Penicillium digitatum SQ2, Aspergillus flavus C1, Aspergillus niger ACCC30005, Trichoderma viride YZ1, Candida tropicalis CICC1630, and Salmonella sp. ZY. For SBM fermentation, the optimal inoculum rate, temperature, and fermentation time of DP-2 were 2.21 × 10
CFU g
, 37 °C, and 48 h, respectively. The fermented soybean meal (FSBM) was cream-colored and glutinous. Its crude protein (CP), soluble protein, and TCA-N content were improved by 13.45%, 12.53%, and 6.37 times, respectively. The glycinin and β-conglycinin content were reduced by 78.00% and 43.07%, respectively, compared with raw SBM.
Bacillus velezensis DP-2 has potential as a starter culture for SBM fermentation. © 2020 Society of Chemical Industry.
Bacillus velezensis DP-2 has potential as a starter culture for SBM fermentation. © 2020 Society of Chemical Industry.
To summarize in a systematic review the current evidence regarding definitions, diagnosis, prevalence, etiology, clinical relevance and impact of surgical treatment for T-shaped uterus not related to diethylstilbestrol (DES) exposure, and to highlight areas on which future research should focus.
AP20187 cost of PubMed, Scopus and EMBASE was performed on 9 April 2020 using the search terms 't-shaped OR t-shape OR infantile OR (lateral indentation) OR (diethylstilbestrol OR DES) AND (uterus OR uterine OR uteri) AND (anomaly OR anomalies OR malformation OR malformations)'. Additionally, the reference lists of the included studies were searched manually for other relevant publications. All studies presenting data on T-shaped uterus not associated with DES exposure and including at least 10 women were considered eligible. Studies regarding DES-related T-shaped uterus were excluded because DES has not been used since 1971. There were no restrictions on language, date of publication or status of publication.
Of 25for this condition. Expectant management should be considered the most appropriate choice for everyday practice until randomized controlled trials show a benefit of intervention. © 2020 International Society of Ultrasound in Obstetrics and Gynecology.
The prevalence, etiology and clinical relevance, with respect to reproductive outcome, of T-shaped uterus remain unclear and there is no consensus on the definition and diagnostic method for this condition. Expectant management should be considered the most appropriate choice for everyday practice until randomized controlled trials show a benefit of intervention. © 2020 International Society of Ultrasound in Obstetrics and Gynecology.
