Gleasonmouritzen5429

This study compared bovine serum albumin (BSA) adsorption onto octacalcium phosphate (OCP) materials prepared from two wet preparations in the absence (w-OCP) and presence (c-OCP) of gelatin. Raman spectroscopy was used to analyze the BSA adsorption onto OCPs in a 150 mM Tris-HCl buffer containing 0.5 mM calcium and inorganic phosphate (Pi) ions at pH 7.4 and at 37°C. The degree of supersaturation of the supernatants after the adsorption was determined by measuring the ion composition. The results showed that BSA adsorption onto w-OCP was higher than that for c-OCP. The calcium ion concentration of the supernatant decreased for both w-OCP and c-OCP, whereas the Pi ion concentration increased, approaching OCP equilibria at different saturation levels. BSA adsorbed even onto c-OCP, which included a small amount of gelatin during c-OCP preparation. These results indicate that the biodegradability of w-OCP and c-OCP may be modulated through interactions with serum proteins.This study evaluates a bioactive titanium membrane with alkali treatment for stimulating apatite formation and promoting bone regeneration. The titanium thin membranes were either treated with NaOH (alkali-group) or untreated (control). Each sample were incubated in simulated body fluid. Subsequently, the composition of the surface calcium deposition, its weight increase ratio, and optical absorbance were evaluated. Then, the bone defect was trephined on the rats calvaria and covered with each sample membrane or no membrane, and the bone tissue area ratio (BTA) and bone membrane contact ratio (BMC) were evaluated. The spherical crystalline precipitates formed in both groups. In the alkali-group after 21 days, the precipitates matured, forming apatite-like precipitates. The alkali-group showed higher Ca and P contents and weight increase ratios than the control. The alkali-group exhibited a higher BMC than the control in the central area. Thus, this novel membrane has high apatite-forming and bone regeneration abilities.To investigate the effect of luting agent type on fracture loads of implant-supported ceramic prostheses in premolar region. Ninety-nine implant-abutment complexes were divided into three different implant-supported prostheses monolithic yttria-partially stabilized zirconia (Y-PSZ) restorations (MPZ specimens), porcelain layered on yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) restorations (PLZ specimens), and monolithic lithium disilicate ceramic restorations (MLD specimens). Implant-supported prostheses were luted with adhesive resin luting agent (RLA), glass ionomer cement (GIC), or zinc phosphate cement (ZPC). For MPZ and MLD specimens, fracture loads were significantly higher for RLA group than for GIC and ZPC groups. For PLZ specimens, fracture loads did not significantly differ in relation to luting agent. Fracture loads were significantly higher for MPZ specimens than for other test specimens, regardless of luting agent. Use of an adhesive resin luting agent is recommended for placement of premolar implant-supported monolithic Y-PSZ and lithium disilicate ceramic prostheses.It has been shown that strontium (Sr) promotes bone formation, reduces bone resorption. In the study, magnetron sputtering method was used for preparing Sr covered on sandblasted and acid-etched (SLA) titanium surface. Surface morphology was examined by scanning electron microscopy (SEM), chemical composition of surface was investigated by X-ray energy dispersive spectrometry (EDS). MC3T3-E1cells were cultured on Sr-SLA and SLA disks. Cell morphology was studied through confocal laser scanning microscope and SEM. Cell proliferation were measured by MTT assay. Degrees of mineralization and alkaline phosphatase (ALP) activity were calculated and compared. SP 600125 negative control mouse EDS and SEM results indicated that Sr ions were successfully loaded in Sr-SLA surface, whereas, Sr-SLA and SLA surfaces demonstrated similar typical isotropic irregular indentations. The MC3T3-E1 cells developed on Sr-SLA surface showed improved morphology, better proliferation as well as greater differentiation.These findings suggest that the modification with Sr incorporated in moderately rough surface has a favorable biocompatibility.In the oral environment dental materials are subject to a wet condition what might in time change their elastic properties. In this article, we evaluated the influence of the storage condition (dry versus wet) on the Young's modulus and the Poisson ratio in compression of three composite materials. The data of the Young's modulus and Poisson ratio published of dental composite materials are not always comparable, due to different test methods and sample dimensions influencing the results. Therefore, we established the degree of exactness of the results out of the test set-up used. Since the present study depicted differences of the properties after dry and wet storage, the elastic properties should be measured after wet storage. The bonding between the matrix and the filler particles showed to have an influence on the elastic properties and on the influence of a wet environment.The purposes of this study were to evaluate the shear bond strength and perform the computational analysis for silane coupling treatments on the adhesion between CAD/CAM composite resin and resin cement. As silane coupling agents, γ-methacryloyloxy propyltrimethoxysilane, 8-methacryloyloxyoctyl trimethoxysilane, and 3-methacryloyloxypropyl trichlorosilane were used. The shear bond strengths were influenced by the silane coupling agent used, its application method, and acid addition. There was no correlation between the contact angle and shear bond strengths. The steric energy difference between a silane coupling agent and its corresponding hydrolyzed trisilanol compound, ∆E, was calculated by the molecular mechanics method. There was a moderate or strong linear correlation between ∆E and shear bond strengths in treatment without acid addition and a weak correlation between them in treatment with acid addition. Computational analysis could suggest the different path of silane coupling treatments of CAD/CAM composite resin in the presence or absence of acid.