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1 ± 8.6, 62.6 ± 14.2 and 54.3 ± 9.9 min, respectively. These difference between all groups, statistically were significant (p = 0.026). However, the clinical efficacy of abomasal emptying rate facilitating by tylosin or ivermectin administration in lambs remains to be determined.Nanoparticles (NPs) have unique properties, leading to their widespread application in industry, consequently increasing their concentration in aquatic ecosystems. Although environmentally significant concentrations are still low, they tend to increase because of the intense use, posing into risk microalgae communities. Microalgae are primary producers that support food chains in aquatic ecosystems; thus factors that interfere with their physiology can be propagated throughout the food web. The present research investigated the effects of copper nanoparticles (Cu-NPs) in the physiology of a cosmopolitan green microalgae, Ankistrodesmus densus. Here, we focused on environmental NPs levels, so an ample Cu-NPs range was used, 0.3-635 μg L-1. Considering that NPs dissolve into the medium releasing their constituent material, free Cu2+ ions were determined and considered as surrogate for NPs concentration, which varied from 2.1 × 10-9 to 8.4 × 10-9 mol L-1. The experiment was based in 72 h Cu-NPs exposure, and to access the physiology of A. densus, we monitored population growth, photochemistry of photosynthesis and the content of cell biomolecules (total proteins, carbohydrates and lipids). The results showed that 2.1 × 10-9 mol L-1 free Cu2+ was enough to decrease growth rate, but 2.5x higher Cu was necessary to affect the photosynthetic parameters. Inorganic carbon fixation rate calculated by absolute electron transport rates was affected. selleck Considering cell biomolecules, total proteins accumulated at 6.5 × 10-9 and kept increasing up to 8.4 × 10-9 mol L-1 free Cu2+. Because this was not related to biomass formation, we suggest a possible association with cell detoxification mechanisms. The most clear finding that emerged from this study is that environmental Cu-NPs concentrations affect vital functions in the green microalgae A. densus. An implication of this is the possibility of facing problems related to a increase of NPs in aquatic ecosystems in the near future.Vasoactive intestinal peptide (VIP) receptor (VPAC1, VPAC2) abundances in the myometrium and functions in the regulation of inflamed uterine contractility in pigs were studied. In the CON group with gilts, only laparotomy was performed. The gilts of SAL- and E. coli-treated groups were administered saline or E. coli into the uterine horns, respectively. The E. coli-induced endometritis resulted in a lesser myometrial relative abundance of VPAC1 and VPAC2 receptor mRNA transcripts and larger abundance of protein for these receptors. In the myometrium, treatment with VIP resulted in a lesser contractility amplitude than in the tissues of the CON- and SAL- and E. coli-treated groups and in frequency in the CON- and E.coli-treated group compared to the period before VIP treatment. Compared to when there was VIP treatment alone, treatment with VPAC1 and VPAC2 receptor antagonists resulted in a lesser inhibitory effect of VIP on contractility amplitude in the myometrium of the CON and SAL-treated groups and there was complete abolishment of the inhibitory VIP effect on frequency of myometrial contractility of the CON group. In the myometrium of E. coli-treated group, treatment with VPAC1 and VPAC2 receptor antagonists resulted in a reversal of the inhibitory effect of VIP on contractility amplitude, while treatment with VPAC2 receptor antagonist resulted in elimination of contractility and a lesser endometrium/myometrium inhibitory effect of VIP on frequency of these contractions. Results indicate VIP functions to decrease myometrial contractility of the inflamed pig uterus by having functions at VPAC1 and VPAC2 receptors.The present study was conducted to evaluate the effects in vitro on oocyte mitochondrial function of C-type natriuretic peptide (CNP) when treatments were imposed before in vitro maturation (IVM). Immature oocytes were either directly matured in vitro for 24 h (Control, no pre-IVM), or cultured in basic medium not supplemented or supplemented with CNP (100 nM) (Control pre-IVM and CNP pre-IVM, respectively) for 6 h, followed by IVM for 24 h. The results indicated treatment with CNP before IVM affected patterns of distribution of mitochondria, increased the mitochondrial content, membrane potential, and decreased the ROS content in cattle oocytes before and after IVM. Furthermore, treatment of immature cattle oocytes with CNP before IVM induced marked increases in the relative abundance of mRNA transcripts and proteins related to mitochondria development and antioxidative defense mechanisms. Treatment with CNP before oocyte IVM also resulted in an enhanced relative abundance of sirtuin-1 (SIRT1) mRNA transcript in cattle oocytes. Taken together, these results provide evidence that treatment of cattle oocytes with CNP before IVM improved mitochondrial function and antioxidant defense mechanisms in cattle oocytes. Findings in the present study provide insights into the potential mechanisms by which CNP has positive effects on oocyte cytoplasmic organelles, specifically mitochondria.The objective of this study was to evaluate effects of dehydration on sperm DNA with the aim of eventually using this method for preserving llama spermatozoa. Two experiments were conducted 1) sperm preservation at 5 °C for 60 days in different hyperosmotic solutions (500, 800, 1000 and 1200 mOsmol/l) (n = 6, replications = 2) and 2) sperm preservation at 5 and -20 °C for 60 days in the same hyperosmotic solutions, with supplementary antibiotics (n = 6, replications = 2). Sperm motility, membrane functional integrity, viability and morphology were evaluated at 0 and 48 h of the preservation period (Experiment 1) and at 30 min and 24 h (Experiment 2). Sperm DNA was evaluated at 0 or 30 min (Experiment 1 and 2, respectively) and on days 7, 14, 21, 30 and 60 of the preservation periods. Motility, membrane functional integrity and viability were less when sperm were dehydrated, while sperm cell morphology was not affected. There was a smaller percentage of sperm with condensed chromatin as duration of the preservation period increased when stored in the different hyperosmotic solutions.