Gaybattle6974

G-Quadruplex (G4)-forming DNA sequences have a tendency to form stable multimeric structures. This can be problematic for studies with synthetic oligodeoxynucleotides. Here, we describe a method that quantitatively converts multimeric intermolecular structures of Pu27 sequence from c-myc promoter into desired monomeric G4 by alkaline treatment and refolding. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.The structural properties and binding motif of a strongly sigma-electron donating N-heterocyclic carbene are investigated on different transition metal surfaces. The examined cyclic (alkyl)(amino) carbene (CAAC) is found to be mobile on surfaces and molecular islands with short-range order could be found at high coverage. By combining scanning tunneling microscopy (STM), X-ray photoelectron spectroscopy (XPS) and density functional theory (DFT) calculations our study highlights how CAACs bind to the surface, which is of tremendous importance to gain understanding for heterogeneous catalysts bearing CAACs as ligands. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.π-Stacked polymers, which consist of layered π-electron systems in a polymer, can be expected to molecular electronic devices. However, the construction of a stable π-stacked structure in a polymer is considerably challenging, because it requires sophisticated designs and precise synthetic methods. Herein, we present a novel π-stacked architecture based on poly(quinolylene-2,3-methylene), bearing alanine derivatives as the side chain, obtained through the living cyclocopolymerization of an o -allenylaryl isocyanide. In the resulting polymer, the neighboring quinoline rings of the main chain form a layered structure with π-π interactions, which is stabilized by intramolecular hydrogen bonds. OSI-774 The vicinal quinoline units form two independent helices and the whole molecule is a twisted tape structure. This structure is established on the basis of UV/CD spectra, theoretical calculations, and atomic force microscopy. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.PURPOSE Iatrogenic bladder injury remains a major challenge. We compared the success and reliability of laparoscopic repair of intraperitoneal bladder rupture in patients who had undergone total laparoscopic hysterectomy. METHODS This retrospective study included patients who underwent total laparoscopic hysterectomy for benign gynaecological cases at a tertiary academic hospital between January 2018 and June 2019. All patient medical records included in the study were reviewed, and the causes, incidence and management of bladder injuries were assessed. RESULTS There were nine patients. The cause of all the ruptures was iatrogenic, and all were intra-operatively detected. In all patients, bladder injuries occurred in the posterior side of the bladder during vesicouterine dissection. Laparoscopic bladder perforation repair was performed successfully in all patients. No major complications had occurred in any patients after surgery. The foley catheters were removed 6.67 ± 0.7 (5-7) days after surgery. CONCLUSIONS If performed by well-trained laparoscopic surgeons, laparoscopic hysterectomy could be the best option for appropriate patients. Nevertheless, patients should be well aware of the potential complications in endometriosis and caesarean cases before the procedure, and care should be paid during dissection. When a urogenital injury is suspected or detected the condition must be adequately identified and proper treatment must be performed to avoid postoperative complications and long-term morbidity. © 2020 John Wiley & Sons Ltd.Promoting and retaining junior faculty are major challenges for many medical schools. High clinical workloads often limit time for scholarly projects and academic development, especially in anesthesiology. To address this, we created the East/West Visiting Scholars in Pediatric Anesthesia Program (ViSiPAP). The program's goal is to help "jumpstart" academic careers by providing opportunities for national exposure and recognition through invited lectures and collaborative opportunities. East/West ViSiPAP benefits the participating scholars, the home and hosting anesthesia departments, and pediatric anesthesia fellowship training programs. By fostering a sense of well-being and inclusion in the pediatric anesthesia community, East/West ViSiPAP has the potential to increase job satisfaction, help faculty attain promotion and reduce attrition. link2 Faculty and trainees are exposed to new expertise and role models. Moreover, ViSiPAP provides opportunities for women and underrepresented in medicine faculty. This program can help develop today's junior faculty into tomorrow's leaders in pediatric anesthesia. We advocate for expanding the concept of ViSiPAP to other institutions in academic medicine. This article is protected by copyright. All rights reserved.COVID-19 was first identified in Wuhan, China and is caused by the novel coronavirus SARS-CoV 2. It has now spread rapidly to over 190 countries and territories around the world and has been declared a global pandemic by the World Health Organization. The virus is spread through droplet transmission and currently has a mortality rate of over 4% globally. The pediatric population has been found to be less susceptible to the disease with the majority of children having milder symptoms and only one pediatric death being reported globally so far. Despite this, strategies need to be put in place to prevent further spread of the virus. We present a summary of the general measures implemented at a large adult and pediatric tertiary hospital in Singapore (National University Hospital) as well as the specific strategies in place for the operating room and pediatric intensive care unit. This article is protected by copyright. All rights reserved.OBJECTIVE Dystonia is a chronic and sometimes progressive neurological disorder causing abnormalities in movement and function. We conducted a preliminary survey to investigate whether people with dystonia experience falls and to identify contributing factors to falls in this population. METHODS An online survey of people with dystonia was conducted in November 2015. Respondents were asked to complete demographic information, three questionnaires (the Falls Self-Efficacy Scale International [FES-I], the Activities-based Balance Confidence Scale [ABC] and the Functional Disability Questionnaire [FDQ]), and to report any falls sustained during the previous 6 months. RESULTS Thirty-nine percent of the 122 respondents reported falling in the previous 6 months and 65% of fallers were diagnosed with dystonia not affecting the lower limbs. Fallers reported lower falls self-efficacy and balance confidence with higher functional disability. Both falling scales correlated with self-reported functional disability. Linear regression analysis for falls prediction revealed the variables FES-I and FDQ accounted for almost 30% of the falls in this dystonia population. CONCLUSION This survey indicates that fear of falling and balance confidence are impaired in people with dystonia, possibly impacting on function and falls. Further investigation into balance, function and falls in this population is required. © 2020 John Wiley & Sons, Ltd.Unvegetated flats occupy a large area in the intertidal zone. However, compared to vegetated areas, the carbon sequestration of unvegetated tidal flats is rarely quantified, even though these areas are highly threatened by human development and climate change. We determined benthic maximum gross primary production (GPPm ), net primary production (NPP) and total respiration (TR) during emersion on seven tidal flats along a latitudinal gradient (from 22.48°N to 40.60°N) in winter and summer from 2012 to 2016 to assess the spatial and temporal variability of carbon dioxide flux. In winter, these processes decreased by 89%-104% towards higher latitudes. In summer, however, no clear trend was detected across the latitudinal gradient. Quadratic relationships between GPPm , NPP and TR and sediment temperature can be described along the latitudinal gradient. These curves showed maximum values of GPPm and NPP when the sediment temperatures reached 28.7 and 26.6°C respectively. TR increased almost linearly from 0 to 45°C. The maximum daily NPP across the latitudinal gradient averaged 0.24 ± 0.28 g C m-2  day-1 , which was only 10%-20% of the global average of NPP of vegetated coastal habitats. Multiplying with the global area of unvegetated tidal flats, our results suggest that the contribution of NPP on unvegetated tidal flats to the coastal carbon cycle is small (11.04 ± 13.32 Tg C/year). If the land cover of vegetated habitats is continuously degraded to unvegetated tidal flats, the carbon sequestration capacity in the intertidal zone is expected to reduce by at least 13.10 Tg C/year, equivalent to 1% of global carbon emissions from land-use change. © 2020 John Wiley & Sons Ltd.OBJECTIVES To evaluate the efficacy of aromatherapy on dental anxiety. link3 METHODS A detailed protocol was registered a priori (PROSPERO CRD42019141093). PubMed, EMBASE, CDSR, CENTRAL, CNKI and grey literature databases were searched from inception to 10 January 2020. Randomised controlled trials (RCTs) and quasi-randomised controlled trials (CCTs) that examined the effects of aromatherapy on dental anxiety, compare to either negative control (i.e. no aromatherapy) or other positive control approaches (e.g. music), were included. The risk of bias of the selected studies was evaluated using the Cochrane Collaboration's tool and ROBINS-I whilst the certainty of evidence was assessed using GRADE. A narrative synthesis was performed, rather than meta-analysis, due to the high level of heterogeneity across studies. RESULTS Eleven RCTs and six CCTs were included. The risk of bias was identified as either high or unclear in RCTs, and moderate to serious in CCTs. Performance bias and detection bias were the most prevalenign and large sample size are warranted to confirm our findings and to confirm the role of aromatherapy in relieving dental anxiety. This article is protected by copyright. All rights reserved.Agricultural soils have tremendous potential to sequester soil organic carbon (SOC) and mitigate global climate change. However, agricultural land use has a profound impact on SOC dynamics, and few studies have explored how agricultural land use combined with soil conditions affect SOC changes throughout the soil profile. Based on a paired soil resampling campaign in the 1980s and 2010s, this study investigated the SOC changes of the soil profile caused by agricultural land use and the correlations with parent material and topography across the Chengdu Plain of China. The results showed that the SOC content increased by 3.78 g C kg-1 in the topsoil (0-20 cm), but decreased in the 20-40 cm and 40-60 cm soil layers by 0.90 and 1.26 g C kg-1 , respectively. SOC increases in topsoil were observed for all types of agricultural land. Afforestation on former agricultural land also caused SOC decreases in the 20-60 cm soil layers, while SOC decreases only occurred in the 40-60 cm soil layer for agricultural land using a traditional crop rotation (i.e., traditional rice-wheat/rapeseed rotation) and with rice-vegetable rotations converted from the traditional rotations. For each agricultural land use, SOC decreases in deep soils only occurred in high relief areas and in soils formed from Q4 (Quaternary Holocene) grey-brown alluvium and Q4 grey alluvium that had a relatively low soil bulk density and clay content. The results indicated that SOC change caused by agricultural land use was depth-dependent and that the effects of agricultural land use on soil profile SOC dynamics varied with soil characteristics and topography. Subsoil SOC decreases were more likely to occur in high relief areas and in soils with low soil bulk density and low clay content. This article is protected by copyright. All rights reserved.