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cessfully. During a follow-up of 50±24 months, 65% had no VT recurrences. Among the seven patients with recurrences, three underwent redo ablation and four, with fewer VT episodes, received appropriate ICD therapy. There were five hospital readmissions due to heart failure decompensation, one patient died in the first week after unsuccessful ablation of a VT storm and three died (stroke and pneumonia) >1 year after ablation.

Catheter ablation based on substrate modification is feasible and safe in patients with frequent VTs and severe LV dysfunction. This approach may be of clinical relevance, with potential long-term benefits in reducing VT burden.

Catheter ablation based on substrate modification is feasible and safe in patients with frequent VTs and severe LV dysfunction. This approach may be of clinical relevance, with potential long-term benefits in reducing VT burden.

The use of mechanical circulatory support is increasing in cases of cardiogenic shock (CS) and high-risk percutaneous coronary intervention (HR-PCI). The Impella® is a percutaneous ventricular assist device that unloads the left ventricle by ejecting blood to the ascending aorta. We report our center's experience with the use of the Impella® device in these two clinical settings.

We performed a single-center retrospective study including all consecutive patients implanted with the Impella® between 2007 and 2019 for CS treatment or prophylactic support of HR-PCI. Data on clinical and safety endpoints were collected and analyzed.

Twenty-two patients were included 12 were treated for CS and 10 underwent an HR-PCI procedure. In the CS-treated population, the main cause of CS was acute myocardial infarction (five patients); hemolysis was the most frequent device-related complication (63.7%). In-hospital, cumulative 30-day and one-year mortality were 58.3%, 66.6% and 83.3%, respectively. In the HR-PCI group, all patients had multivessel disease (mean baseline SYNTAX I score 44.1±13.7). In-hospital, 30-day and one-year mortality were 10.0%, 10.0% and 20.0%, respectively. There were no device- or procedure-related deaths in either group.

The short- and long-term results of Impella®-supported HR-PCI were comparable to those in the literature. In the CS group, in-hospital and short-term outcomes were poor, with high mortality and non-negligible complication rates.

The short- and long-term results of Impella®-supported HR-PCI were comparable to those in the literature. In the CS group, in-hospital and short-term outcomes were poor, with high mortality and non-negligible complication rates.

Heart failure (HF) secondary to acute myocardial infarction (AMI) is still a worldwide problem with a high mortality rate. The current study aimed to explore early and reliable predictive biomarkers of HF following AMI.

The gene expression profile GSE59867 was downloaded from GEO. Array data from peripheral blood mononuclear cells (PBMCs) was used from 46 control patients and 111 patients with AMI at four time points (i) first day of AMI; (ii) 4-6 days after AMI; (iii) one month after AMI; and (iv) six months after AMI. Among the 111 AMI patients, nine with HF and eight without HF were studied. CIBERSORT was used to analyze the relative proportions of immune cells in PBMCs. The proportions of immune cells in different groups were compared. Differentially expressed genes (DEGs) were analyzed with R language packages.

The percentages of monocytes and neutrophils increased significantly on the first day of AMI, and then decreased gradually. The percentage of regulatory T cells increased significantly 4-6 d in the development of HF following AMI.

Ventricular arrhythmias are caused by scar tissue in patients with ischemic dilated cardiomyopathy. The gold standard imaging technique for detecting scar tissue is magnetic resonance imaging (MRI). However, MRI is not feasible for use as a screening test, and also cannot be used in patients who have received an implantable cardioverter-defibrillator (ICD). In this study, we aimed to assess the association between levels of galectin-3 (Gal-3), which is known to be secreted by scar tissue, and the history of ventricular arrhythmias in patients with ischemic dilated cardiomyopathy who received an ICD.

Nineteen healthy controls and 32 patients who had previously undergone VVI-ICD implantation due to ischemic dilated cardiomyopathy were enrolled in the study. Patients were divided into three groups the first group including patients who had received no ICD therapies, the second including patients with arrhythmia requiring therapies with no arrhythmia storm, and the third including patients who had arrhythmia storm. We assessed the association between Gal-3 levels and the history of ventricular arrhythmias in these patients.

Gal-3 levels were significantly higher in the patient groups than in the control group (p<0.01). Gal-3 levels of patients with arrhythmias requiring ICD therapies were significantly higher than in patients with ICD not requiring therapies (p=0.02). They were also higher in patients with a history of arrhythmia storm than in patients without shocks (p=0.05). Receiver operating curve analysis showed with 84% sensitivity and 75% specificity that Gal-3 levels over 7 ng/ml indicated ventricular arrhythmia that required therapies.

Gal-3 may be used to further improve risk stratification in patients with ischemic cardiomyopathy who are more prone to developing life-threatening arrhythmias.

Gal-3 may be used to further improve risk stratification in patients with ischemic cardiomyopathy who are more prone to developing life-threatening arrhythmias.

The Internet is a fundamental aspect of health information. However, the absence of quality control encourages misinformation. We aim to assess the relevance and quality of acute myocardial infarction videos shared on YouTube (www.youtube.com) in Portuguese.

We analyzed 1,000 videos corresponding to the first 100 search results on YouTube using the following terms (in Portuguese) "cardiac + arrest"; "heart + attack"; "heart + thrombosis"; "coronary + thrombosis"; "infarction - brain", "myocardial + infarction" and "acute + myocardial + infarction". Irrelevant (n=316), duplicated (n=345), without audio (n=24) or non-Portuguese (n=106) videos were excluded. Included videos were assessed according to source, topic, target audience and scientific inaccuracies. Quality of information was assessed using The Health on the Net Code (HONCode from 0 to 8) and DISCERN (from 0 to 5) scores - the higher the score, the better the quality.

242 videos were included. The majority were from independent instructors (n=95, 39.0%) and were addressed to the general population (n=202, 83.5%). One third of the videos (n=79) contained inaccuracies while scientific society and governmental/health institution videos had no inaccuracies. The mean video quality was poor or moderate; only one video was good quality without any inaccuracies. Governmental/health institutions were the source with the best quality videos (HONCode 4±1, DISCERN 2±1).

One third of the videos had irrelevant information and one third of the relevant ones contained inaccuracies. The average video quality was poor; therefore it is important to define strategies to improve the quality of online health information.

One third of the videos had irrelevant information and one third of the relevant ones contained inaccuracies. The average video quality was poor; therefore it is important to define strategies to improve the quality of online health information.A novel kind of phosphonate functionalized polythiophene microsphere was designed and fabricated via Friedel-Crafts reaction. Diethyl (thiophen-2-ylmethyl) phosphonate (DTYP) and thiophene were co-polymerized by Fe (III) catalysis, without any surfactant, stabilizer and initiator. Functional phosphonate group was directly introduced into the microsphere without redundant modification steps. The adsorption amount of the as-synthesized microsphere, Ti-poly(Th-co-DTYP), was as high as 66.7 mg/g, which was higher than that of commercial Ti4+-IMAC microsphere (49.7 mg/g). PF2545920 The microsphere was explored on the specific capture of phosphopeptides from either tryptic digests of milk or HeLa cell protein. As a result, 88 of unique phosphopeptides mapping to 21 phosphoproteins were identified from 150 μg of milk tryptic digest after enrichment, and a total of 2534 unique phosphopeptides mapping to 1087 phosphoproteins was identified from HeLa cell. It is expected that such a robust and facile approach will be explored in other functional microspheres to be commercialized in the future.In this work, a novel signal on/off ratiometric electrochemical sensor for the selective detection of chlorpromazine (CPZ) was developed. The sensor was constructed by electrodepositing dual-monomer molecularly imprinted polymer (DMMIP) film on the surface of Pt/Co3O4 nanoparticles modified glassy carbon electrode, using CPZ as template molecule, methylene blue and catechol as functional monomers. The copolymerization of two monomers increased the diversity of functional groups for binding template molecules, and enhanced stability. The quantitative detection of CPZ was performed by differential pulse voltammetry, using the peak current of poly (methylene blue) as reference signal and the peak current of CPZ as indicating signal. The results showed that the developed DMMIP sensor not only possessed high selectivity and sensitivity, but also exhibited satisfactory anti-interference ability. Under the optimum conditions, a linear detection range of 0.005-9 μmol L-1 (R2 = 0.9962) was obtained, and the limit of detection was 2.6 nmol L-1. Moreover, the sensor showed good reproducibility and stability toward CPZ detection. It was applied to detect CPZ in serum and pharmaceutical samples, and satisfactory recoveries (ranging from 95.3% to 108.0%) were achieved.Spectral imaging (SI) in analytical chemistry is widely used for the assessment of spatially distributed physicochemical properties of samples. Although massive development in instrument and chemometrics modelling has taken place in the recent years, the main challenge with SI is that available sensors require extensive system integration and calibration modelling before their use for routine analysis. Further, the models developed during one experiment are rarely useful once the system is reintegrated for a new experiment. To avoid system reintegration and reuse calibrated models, this study presents an intelligent All-In-One SI (ASI) laboratory system allowing standardised automated data acquisition and real-time spectral model deployment. The ASI system supplies a controlled standardised illumination environment, an in-built computing system, embedded software for automated image acquisition, and model deployment to predict the spatial distribution of sample properties in real-time. To show the capability of the ASI framework, exemplary cases of fruit property prediction in different fruits are presented. Furthermore, ASI is also benchmarked in performance against the current commercially available portable as well as high-end laboratory spectrometers.Environment-sensitive fluorescent probes have always been as forceful tools to understand the pathophysiological processes of relevant diseases. In this work, a new fluorescent probe with typical D-π-A structure was designed and showed high sensitivity to polarity and viscosity changes. DPAR could selectively detect human serum albumin (HSA) with turn-on orange emission in aqueous PBS buffer (pH 7.4), which showed advantages such as rapid response (4 min), high sensitivity (LOD 0.98 μg/mL). Therefore, it was successfully used for achieving HSA levels in urine samples and HSA imaging in HeLa cells. DPAR also exhibited the capability to recognize the cancer cells over the normal cells by lower polarity guided lipid droplets (LDs) imaging (in green emission channel). The detection mechanism for HSA and cancer diagnosis was convinced that DPAR encountered the lower-polarity and higher-viscosity microenvironment, resulting in the confinement of the TICT process and intramolecular rotation. These facts showed that DPAR had good application prospects in environment-related biomedical research and clinical diagnosis.