Fraserrobb4273

Expression analysis of DND1 was found to be significantly overexpressed in gastric cancer tissues and cell lines. Suppression of DND1 suppressed the proliferation of gastric cancer cells. Wound healing and transwell assay revealed that miR-24 overexpression also inhibited the migration and invasion and also enhanced the chemosensitivity of the SNU1 gastric cancer cells. Conclusion Taken together, miR-24 may prove to be an important therapeutic target for the treatment of gastric cancer and warrants further studies.1Department of General Surgery, The No.1 Hospital of Shijiazhuang, Shijiazhuang, China.Purpose To explore the efficacy and safety of apatinib (an anti-angiogenic drug) combined with S-1 (a fluorouracil drug) in the third-line chemotherapy for advanced gastric cancer, and to analyze the factors influencing the prognosis. Methods Eighty-four patients with advanced gastric cancer, who did not respond to second-line or above chemotherapy and were treated in our hospital were enrolled and divided into Apatinib+S-1 group (n=42) and S-1 group (n=42), based on different treatments applied. Next, the clinical responses and adverse reactions of patients were observed and recorded. The patients were followed up through the outpatient service and telephone to record their survival and disease progression. Additionally, the factors affecting the prognosis of patients were analyzed. Results The objective response rate (ORR) and disease control rate (DCR) in the Apatinib+S-1 group were 9.5% (4/42) and 71.4% (30/42), respectively, which were significantly higher than those in the S-1 group. The main adverse rects after treatment with apatinib combined with S-1 in the third-line therapy, whose PFS is notably better than those treated with S-1 alone, and they are tolerant to adverse reactions. Highly differentiated tumors and post-treatment proteinuria and hand-foot syndrome are predictable factors for the PFS of patients.Purpose To explore the effects of aspirin (ASP) on the proliferation and apoptosis of HepG2 hepatocellular carcinoma (HCC) cells via the Wnt/β-catenin signaling pathway. Methods Human HCC cells were cultured and treated with ASP at different concentrations. Cell proliferation was determined with cell counting kit-8 (CCK-8) and colony formation, and the rate of apoptosis was measured by flow cytometry. Western blotting (WB) and quantitative polymerase chain reaction (qRT-PCR) assays were used to assess the changes in the expression levels of related proteins. Results ASP showed a time-and concentration-depented inhibitory effect on HepG2 cell proliferation. The number of colonies formed in ASP-treated HCC cells was significantly lower than in control cells. For HCC cells treated with ASP, the apoptosis rate enhanced with the increase of ASP concentration. The expression levels of TCF4 and LEF1, key molecules of the Wnt/β-catenin signaling pathway, were lowered in HCC cells treated with 4 mM ASP, and the nuclear translocation of β-catenin was weakened. The β-catenin activator exerted a negative influence on the anticancer effect of ASP. Conclusions ASP inhibits the proliferation and promotes the apoptosis of HCC cells through the Wnt/β-catenin signaling pathway.Purpose To investigate the level of long noncoding ribonucleic acid (lncRNA) MINCR in hepatocellular carcinoma (HCC), and to further investigate whether it can promote the development of HCC through modulating microRNA-107/β-catenin. Methods MINCR level in 52 pairs of HCC tumor tissues as well as adjacent tissues and HCC cell lines was detected by quantitative real-time polymerase chain reaction (qRT-PCR). MINCR knockdown model was constructed using lentivirus in the carcinoma cell lines. Furthermore, cell counting kit-8 (CCK-8), plate cloning and apoptosis assay were used to analyze the effect of MINCR on the biological function of HCC cells. Finally, cell recovery experiment was performed to explore its potential mechanism and the association between MINCR and microRNA-107/β-catenin. Results qPCR results showed that the level of MINCR in HCC was remarkably higher than that in adjacent tissues, and the difference was statistically significant. Compared with patients with low level of MINCR, patients with high level of MINCR had lower overall survival rate. Similarly, the cell proliferation ability of sh-MINCR group was remarkably decreased while the apoptosis ability was oppositely increased when compared with the short hairpin RNA (shRNA) group. In addition, studies have demonstrated that the levels of microRNA-107 and MINCR in HCC tissues were negatively related. In our study, dual-luciferase reporting assay verified that MINCR can be targeted by microRNA-107 through certain binding site. In addition, MINCR was confirmed to be able to further regulate the malignant progression of HCC through microRNA-107/β-catenin. Conclusions The level of MINCR was remarkably increased in HCC, which was associated with poor prognosis of HCC patients. T0901317 Moreover, MINCR was capable of promoting the proliferation and inhibiting apoptosis of liver cancer cells via regulating microRNA-107/β-catenin.Purpose To explore the efficacy and reliability of enhanced recovery after surgery (ERAS) applied in the perioperative period of precise hepatectomy for hepatocellular carcinoma (HCC). Methods The propensity score matching and a retrospective cohort study were employed. The clinical and pathological data of 122 hepatocellular carcinoma (HCC) patients with surgical indications admitted to our hospital from March 2014 to March 2016 were collected. These 122 patients were subjected to propensity score matching and divided into ERAS group and Control group. The surgical situation, postoperative recovery [postoperative alanine aminotransferase (ALT), total bilirubin (TBiL) and C-reactive protein (CRP) levels], postoperative complications, postoperative hospital stay, hospitalization costs and patient satisfaction score were observed and compared between the two groups. All patients were followed up to record their postoperative survival. Results The average drainage tube removal time, bowel sound time, postoperative flatus time and postoperative hospital stay of patients were overtly shorter in ERAS group than in Control group.