Francispierce7813

74 °C. Using 100, 300, and 500 g L-1 of d-fructose as substrate, 25.6, 74.4, and 115 g L-1 of d-mannose were produced respectively, corresponding to a conversion rate of 25.6 %, 24.8 %, and 23.0 % under optimal conditions. Taken together, our results provide evidence for a promising candidate d-LIase for producing d-mannose directly from d-fructose.A novel prolyl endopeptidase from Stenotrophomonas maltophilia, SmPEP, was discovered and characterized. The specific activity of the recombinant SmPEP expressed by Escherichia coli BL21 (DE3), was 68.3 U/mg at pH 8.0 and 37 °C. In order to improve the substrate specificity for long-chain peptide, rational design was applied based on the structure constructed by homology modeling. find more Inter-domain sites within the β-propeller domain were chosen for the mutation to weaken the inter-domain interaction and form an open conformation for long-chain substrate entering into the active site. The substrate specificity on a designed long-chain substrate, PQPQLPYPQPQLP, of the mutants F263A and E184 G increased 8.77 and 5.75 times respectively versus wild-type. After the saturated mutation of the both sites, the reactive rate of mutant F263 V on 13-mer peptide was 10.2 times higher than that of the wild-type. Then the mutant F263 V was used in the hydrolysis of casein, and the ACE inhibitory activity of the hydrolysate was significantly improved compared with wild type enzyme, which verified the efficiency of the design strategy.Ruminiclostridium josui Fae1A is a modular enzyme consisting of an N-terminal signal peptide, family-1 carbohydrate esterase module (CE1), family-6 carbohydrate-binding module (CBM6), and dockerin module in that order. Recombinant CE1 and CBM6 polypeptides were collectively and separately produced as RjFae1A, RjCE1, and RjCBM6. RjFae1A showed higher feruloyl esterase activity than RjCE1 towards insoluble wheat arabinoxylan, but the latter was more active towards small synthetic substrates than the former. This suggests that CBM6 in RjFae1A plays an important role in releasing ferulic acid from the native substrate. RjCBM6 showed a higher affinity for soluble wheat arabinoxylan than for rye arabinoxylan and beechwood xylan in native affinity polyacrylamide gel electrophoresis. Isothermal titration calorimetry analysis demonstrated that RjCBM6 recognized a xylopyranosyl residue at the nonreducing ends of xylooligosaccharides. Moreover, it showed exceptional affinity for 23-α-l-arabinofuranosyl-xylotriose (A2XX) among the tested branched arabinoxylooligosaccharides. Fluorometric titration analysis demonstrated that xylobiose and A2XX competitively bound to RjCBM6, and both bound to the same site in RjCBM6. RjCBM6's preference for the xylopyranosyl residue at the nonreducing end of xylan chains explains why the positive effect of CBM6 on RjFae1A activity was observed only during short incubation but not after extended incubation.Giant cell tumor (GCT) is a benign but locally aggressive neoplasm generally located in the epiphysis and metaphysis of the long bones. Its occurrence in the bones of the hand is rare. Giant cell tumors occurring in the hand account for only 2% of all the GCTs reported. The epimetaphyseal region of short long bones is a common site of GCTs in the hand; however, a phalangeal GCT originating in the distal phalanx is extremely rare. We present the clinical, radiological and histopathological findings of a case of GCT of the distal phalanx of the left ring finger presenting with a fungating mass and treated with en bloc resection by disarticulation of the distal interphalangeal joint.Energy storage and returning prosthetic feet do not provide a well-defined articulation point compared to the human ankle. Calculation of user relevant parameters, such as ankle power, requires such a joint center point when using traditional mechanical models. However, shortcomings of current calculation methods result in some errors. The aim of this case study was to compare conventional ankle joint calculations to a functional joint center (FJC) using data collected on a roll-over test machine and in a motion lab during dissimilar walking tasks. Three prosthetic feet were evaluated on a roll-over test machine. Then, two trans-tibial amputees were each fitted with the same three prosthetic feet matching their weight and activity category. Kinematic data were collected during walking on level ground, as well as up and down a slope. The FJC during the stance phase of gait was calculated for each test method and compared with outcomes using conventional methods. The location of the FJC was generally anterior anformative measure of the prosthetic foot's response to the user's needs. Furthermore, prosthetists could use this metric in clinical practice to better appreciate amputee feedback and perception. FJC provides an alternative center during calculation of ankle power using standard methods.The aim of this study was to investigate the residual limb stress of a transfemoral amputee's Compression/Release Stabilized (CRS) socket by finite elemental modelling. The model was constructed from magnetic resonance images of the left residual limb of a 48-year-old male transfemoral amputee. Two conditions were simulated. In the donning condition, the prosthetic socket under the residual limb moved proximally until it reached the required donned position. The weight-bearing condition was subsequently simulated by applying body weight (800N) at the femoral head while keeping the distal end of the socket fixed. The maximum contact pressure was concentrated at the proximal anterior-medial regions of the residual limb surfaces in both conditions. In the donning condition, the maximum von Mises stress and the maximum contact pressure were 277.7 kPa and 254 kPa respectively. The respective values were 191.9 kPa and 218.5 kPa when body weight was applied. The stress and contact pressure did not exceed the suggested threshold value of pain. Our findings provide important biomechanical information on the CRS socket that may help future design optimization.Background Primary cutaneous CD8+ aggressive, epidermotropic, cytotoxic T-cell lymphoma is a rare disease with a poor prognosis. Herein we report a new case, with facial lesions, which was difficult to diagnose. Patients and methods A 39-year-old woman was hospitalized for ulcerated nodules on the face that had been developing rapidly for 8 weeks. She had visited Djerba, Tunisia, 3 months earlier. No abnormalities were found on previous routine blood tests. Histopathological analysis of a skin biopsy had revealed non-specific lymphocytic infiltrate. Various therapies, including amoxicillin/clavulanic acid, valaciclovir, corticosteroids, colchicine and doxycycline, proved ineffective. Screening of the cutaneous sample for leishmaniasis proved positive using PCR but negative by direct examination and culture. Treatment was initiated with meglumine antimoniate. A further cutaneous biopsy revealed diffuse lymphocytic proliferation and led to a diagnosis of cutaneous CD8+ aggressive, epidermotropic, cytotoxic T-cell lymphoma.