Floydholm0232

Regardless of the chosen density, for a polyculture of Nile tilapia and bullfrog tadpoles, ideal conditions would be stocking tilapia fry weighing 50% of the weight initial tadpoles and the proportion of one tilapia for seven tadpoles.This study aimed to evaluate the effects of the dietary supplementation Curcuma longa hydrolate on Nile tilapia (Oreochromis niloticus) reared in a recirculation system. Hemato-immunological parameters, growth performance, nitrogen and phosphorus retention, as well as body composition and its interaction with the intestinal microbiota, were studied. Nile tilapia fingerlings (120) were distributed randomly in 8 polyethylene tanks (40 L). The experimental units were divided into two treatments, in quadruplicate commercial diet supplemented with 2.5% of C. longa hydrolate and commercial diet without supplementation (control). find more After 45 days, the treatment supplemented hydrolate showed higher survival than the control group, 95.25% and 82.22%, receptively. In the blood profile, fish supplemented with hydrolate had a higher count of total leukocytes and neutrophils, as well as mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration, than control group. The hydrolate group showed a substantial increase in the relative abundance of Cetobacterium and Romboutsia, as well as lower diversity in gut microbiota. The dietary addition of C. longa hydrolate for Nile tilapia seems to have a beneficial effect on gut microbiota, in addition to a likely positive effect on the physiological performance of Nile tilapia by maintaining intestinal homeostasis and promoting survival in reared conditions.The Agaricomycetes fungi produce various compounds with pharmaceutical, medicinal, cosmetic, environmental and biotechnological properties. In addition, some polysaccharides extracted from the fungal cell wall have antitumor and immunomodulatory actions. The aim of this study was to use genetic modification to transform Schizophyllum commune and identify if the phenotype observed (different from the wild type) resulted in changes of the cell wall polysaccharides. The plasmid pUCHYG-GPDGLS, which contains the Pleurotus ostreatus glucan synthase gene, was used in S. commune transformations. Polysaccharides from cell wall of wild (ScW) and mutants were compared in this study. Polysaccharides from the biomass and culture broth were extracted with hot water. One of the mutants (ScT4) was selected for further studies and, after hydrolysis/acetylation, the GLC analysis showed galactose as the major component in polysaccharide fraction from the mutant and glucose as the major monomer in the wild type. Differences were also found in the elution profiles from HPSEC and NMR analyses. From the monosaccharide composition it was proposed that mannogalactans are components of S. commune cell wall for both, wild and mutant, but in different proportions. To our knowledge, this is the first time that mannogalactans are isolated from S. commune liquid culture.The objective of the study was to compare the richness and diversity of ant assemblages in an agroecological system under peach orchard, conventional system under peach orchard cultivation and native vegetation in rural properties located in a Pampa Biome. The study was conducted in four samplings in 2017 1st and 09th March (summer); 24th and 31st July (winter); and four samplings in 2018 23rd and 30th January (summer); 31st July and 07th August (winter). Pitfall traps were used. The assemblages were characterized and compared using richness, number of occurrences of ants, Shannon diversity (H'), equitability, rarefaction analysis and Chao 1. The association of the species with the samples was evaluated by a Principal Component Analysis (PCA). The agroecological system had the highest number of occurrences, while the conventional orchard the lowest number. Richness and abundance were greatest during the summer. The conventional peach orchard obtained the lowest H' for both seasons when compared to the agroecological orchard and native vegetation. The PCA explained 77.40% of the occurrence of ants in the environments and in the seasons. The results found demonstrated that conservationist systems tend to harbor greater wealth and diversity of ant assemblages, as well as occurring in native áreas.Bacteria of the genus Bacillus sp. present the potential for inhibiting various pathogens, making them a promising starting point in the search for new antimicrobial substances. In this study, bacteria were isolated from sediment samples from humid areas of a Natural Conservation Unit in the state of Rio Grande do Sul, Brazil. The isolate Bacillus sp. sed 1.4 was selected for production of antimicrobial activity, and was characterized by MALDI-TOF and 16S rDNA sequencing. Phylogenetic analysis showed that Bacillus sed 1.4 was closely related to Bacillus altitudinis and Bacillus pumilus. The cell-free supernatant was partially purified using ammonium sulfate precipitation, gel filtration chromatography (Sephadex G-200) and an ultrafiltration membrane. Partial purification resulted in specific activity of 769.23 AU/mg, with a molecular mass of approximately 148 kDa. This antimicrobial substance showed stability at 100°C for 5 min, and was inactivated by proteolytic enzymes. An antimicrobial effect against Listeria species was observed. Considering the importance of the Listeria genus in the area of food safety, this antimicrobial activity should be further explored, specifically in the field of dairy products and with a focus on food biopreservation studies.Bioremediation techniques like bioaugmentation and/or biostimulation are an economical and environmentally friendly procedure which emerged as the most advantageous methodology for treatment of contaminated sites by oil spills pollutants. This research uses a tropical soil contaminated with oil based drilling fluids (OBMs) and drill cuttings were evaluating at laboratory scale. Seven treatments were implemented separately (C) control; (A) natural attenuation; (B) compost (Bs) nutrients; (BsT) nutrients and tween 80; (BsTL) nutrients, tween 80, leonardite, and (BL) nutrients, tween 80, leonardite and d-limonene. For three months, changes in Total Petroleum Hydrocarbons (TPH) soil microbial counts and activity were monitored as indicators of biodegradation. In order to evaluate the efficiency of treatments in the microcosm experiments. After 90 days of incubation hydrocarbon biodegradation is 76.2% (C), 28.6% (A), 76.2% (B), 66.7% (Bs), 83.3% (BsT), 69% (BsTL) and 88.1% (BL), respectively. Scanning electron microscopy (SEM) of OBMs evidenced absence of heavy metals. Biodiversity analysis showed a decrease in bacterial diversity and a rise in tolerant genus of hydrocarbons such as Nocardiodes, Streptomyces, Dietzia and Advenella. The co-substrate and stimulants had synergistic effect on the biological degradation of hydrocarbons. This research suggests that the implementation of bioaugmentation and biostimulation methods will be used a larger scale in contaminated sites.We present the first formal record of the twig-girdler Psyllotoxus griseocinctus Thomson, 1868 (Cerambycidae Lamiinae Onciderini) attacking the rose, Rosa sp. (Rosaceae) in Southern Brazil. We provide photographs of the damage P. griseocinctus causes to the plant, as well as morphological descriptions of the immature stages and molecular diagnostic tools to identify this species. Additionally, we provide a modelled map with the known and potential distribution of P. griseocinctus and new host records for three plant species the common walnut tree, Juglans regia L. (Juglandaceae); the cambucá tree, Plinia edulis (Vell.) Sobral (Myrtaceae); and the cherry tree, Prunus cf. serrulata Lindl. (Rosaceae).Hohenbergia lanata Pereira & Moutinho is an endemic bromeliad of the State of Bahia (Brazil), occurring in an altitudinal transition forest between Caatinga dry woodland and Atlantic Forest. The type material was collected by Roberto Burle-Marx, who introduced it into cultivation in his gardens, but lacking the accurate provenance, only mentioning that it was collected between Milagres and Amargosa municipalities. It was later described by Pereira & Moutinho based on a cultivated specimen. In this work, we present new specimens found in natural populations in an area that coincides with the type locality description, enabling us to expand the description of the species, as well as to provide images of the living plants in the field, adding information about its habitat. The herbarium collections revision and the new population found in the field enabled us to propose an updated conservation status and to better characterize the habit, plant architecture, and to add new morphological comparisons and geographical distribution.We investigated ten honey samples from the Discovery Coast of Bahia, Brazil, to confirm the three predominant flower sources indicated by regional beekeepers Brazilian peppertree - Schinus terebinthifolia Raddi; eucalyptus - Eucalyptus L'Hér. spp.; and velame - Croton L. spp.. The honeys were collected in five Southern Bahia municipalities - Belmonte, Eunápolis, Guaratinga, Itabela and Porto Seguro. The samples underwent acetolysis and, after slides mounting, the pollen types were identified and counted to determine the frequency classes. The pollen spectra revealed 31 pollen types in 27 genera and 17 families. The palynological analysis confirmed the predominant eucalyptus flower source indicated for four honey samples from Belmonte, Eunápolis, Itabela and Porto Seguro. Three honey samples previously indicated with predominant eucalyptus and velame flower sources had no predominant pollen. Eucalyptus predominated as the main flower source for another three samples previously indicated by beekeepers as Brazilian peppertree and velame. We conclude that regional honeys marketed as having a predominant flower source may have distinct botanical origin. This demands further research aimed at reviewing which bee plants provide resources for monofloral, bifloral and plurifloral honeys in the Bahian Discovery Coast.

This study aims to evaluate the ability of deep learning algorithms to detect and grade prostate cancer (PCa) in radical prostatectomy specimens.

We selected 12 whole-slide images of radical prostatectomy specimens. These images were divided into patches, and then, analyzed and annotated. The annotated areas were categorized as follows stroma, normal glands, and Gleason patterns 3, 4, and 5. Two analyses were performed i) a categorical image classification method that labels each image as benign or as Gleason 3, Gleason 4, or Gleason 5, and ii) a scanning method in which distinct areas representative of benign and different Gleason patterns are delineated and labeled separately by a pathologist. The Inception v3 Convolutional Neural Network architecture was used in categorical model training, and a Mask Region-based Convolutional Neural Network was used to train the scanning method. After training, we selected three new whole-slide images that were not used during the training to evaluate the model as our test dataset. The analysis results of the images using deep learning algorithms were compared with those obtained by the pathologists.

In the categorical classification method, the trained model obtained a validation accuracy of 94.1% during training; however, the concordance with our expert uropathologists in the test dataset was only 44%. With the image-scanning method, our model demonstrated a validation accuracy of 91.2%. When the test images were used, the concordance between the deep learning method and uropathologists was 89%.

Deep learning algorithms have a high potential for use in the diagnosis and grading of PCa. Scanning methods are likely to be superior to simple classification methods.

Deep learning algorithms have a high potential for use in the diagnosis and grading of PCa. Scanning methods are likely to be superior to simple classification methods.