Finkstein9108

Selectivity and repeatability studies were carried out in real blood samples and the recovery was higher than 96%. In conclusion, dCas9-sgRNA was effectively immobilized and optimized on GPHOXE as the selective biorecognition receptor of this ultrafast impedimetric biosensor. The CRISPR-dCas9 powered impedimetric system showed good selectivity, high repeatability and good recovery properties. This is the first literature to report the use of CRISPR/Cas technology as a label-free tool that can be used in an impedimetric system for detection of ctDNA's.Phosphorylation is a common process for regulating protein functions. Studies have confirmed that many human diseases are associated with the abnormal phosphorylation of proteins. Hence, uncovering the phosphorylation state of proteins turns to be of much importance for biomedicine and clinical practice. In this work, we report a simple but efficient colorimetric sensor array for the quantification and identification of phosphorylated proteins by using a Zr-based MOF as a peroxidase mimic. Thanks to its unique dipyridyl-based ligands, the proposed MOF is able to exhibit favorable catalytic activity to stimulate the chromogenic reaction of H2O2 and 3,3',5,5'-tetramethylbenzidine. When phosphorylated proteins are in presence, they can anchor onto the nanozyme surface via the strong interaction between phosphate groups in proteins and Zr nodes in the MOF, resulting in the inhibition of the nanozyme's activity and the suppression of the chromogenic reaction. Based on this principle, our colorimetric sensor array enabled the facile quantification of phosphorylated proteins. Given that proteins with different phosphorylation states would affect the catalytic activity of the MOF nanozyme in different degrees, we further integrated the array with principal component analysis for the successful identification of phosphorylated and non-phosphorylated proteins.Detection of circulating tumor DNA (ctDNA) is important approach to risk stratification and treatment response monitoring of cancer patients, but current method lacks of enough sensitivity and repeatability. The paper repors shape-controlled synthesis of gold nanocrystals via reduction of HAuCl4 with ascorbic acid. The synergy of CTAC, KBr, KI and L-glutathione creates urchin-like gold nanocrystals (U-Au) with more exposed high-index facets. Preparation of electrochemical sensing platform for ctDNA involves modification of U-Au-multiple graphene aerogel for target DNA-induced recycle amplification. DNA probe 1 (P1) with methylene blue (MB) hybridizes with DNA probe 2 with ferrocene (Fc) to form duplex DNA, which was attached to U-Au through Au-S bond. The ctDNA hybridizes with hairpin DNA 1 to open hairpin structure, triggering target DNA-induced recycle. Utilization of target DNA-induced recycling allows one target DNA to approach many MB probes to electrode surface and to leave many Fc probes from electrode surface, promoting significant signal amplification. The detection signal is enhanced by catalyzed redox of Fc and MB. Electrochemical response increases with ctDNA concentration from 0.1 to 1 × 106 fM with detection limit of 0.033 fM. The biosensor provides ultrahigh sensitivity, specificity and stability and was successfully applied in detection of ctDNA in human blood.In this experiment, a highly effective electrochemical sensor based on a molecularly imprinted polymer has been developed for ultrasensitive detection of dimetridazole. The sensor was made by incorporating of dimetridazole as a template molecule during the electropolymerization of poly-arginine on a glassy carbon electrode. The modified electrode GCE/P-Arg@MIP was characterized by voltammetric and microscopic techniques. Differential pulse voltammetry method was used to detect target analyte under the optimum condition. The DPV response to dimetridazole was linear at 0.1 × 10-9 to 10 × 10-6 mol L-1 (R2 = 0.996), with a method detection limit (S/N = 3) of 0.1 × 10-9 mol L-1. Moreover, the proposed sensor shows satisfactory recovery ranges for the determination dimetridazole in commercially available egg, milk and honey samples.Many polymer decorated/modified 2D nanomaterials have been developed as enhanced drug delivery systems and photothermal theranostic nanoagents. However, few reports describe the use of these novel nanomaterials as nanoplatforms for biomolecule sensing. Selleck BTK inhibitor Herein, we used calcium-cation-doped polydopamine-modified (PDA-modified) 2D black phosphorus (BP) nanosheets (BP@PDA) as a sensing nanoplatform for the detection of nucleic acids and proteins in complex biological samples. Fluorescent-dye-labeled single-strand DNA aptamer/probes are adsorbed by the Ca2+-doped BP@PDA mediated by calcium-cation coordination. The PDA coating enhances the stability of the inner BP, provides binding sites to DNA nucleobases, and quenches fluorescence. Without any chemical conjugation, this sensing nanoplatform selectively and specifically detects protein (human thrombin, linear range 10-25 nM, detection limit 0.02 nM), single-strand DNA (linear range 1-10 nM, detection limit 0.52 nM) in 1% serum diluted samples, and senses intracellular mRNAs (C-myc, and actin) in living cells. The nanoplatform exhibits the advantages of both the 2D nanomaterial (BP) and the coating polymer (PDA), naturally enters living cells unaided by transfection agents, resists enzymatic lysis and shows high biocompatibility. This nanoplatform design contributes towards future biomolecule analytical method development based on polymer decorated/modified 2D nanomaterials.Objective To compare the main academic characteristics and contents of the different master's programs in public health currently offered in Spain. Method A systematic search has been carried out in the Register of Universities, Centers and Degrees of the Ministry of Education, Culture and Sports. The main academic characteristics and the contents (mandatory and optional) of the programs of 11 official master's degrees with the renewed accreditation in 2018 were analyzed based on the data published on the universities' web pages. Results Most programs are 60 ECTS (European Credit Transfer and Accumulation System), face-to-face, taught in Spanish, include in their curriculum the completion of a master's thesis but not professional practices. Only contents related to statistics and basic epidemiology, and the training activity of master's thesis, are offers by all programs. Conclusions The majority of public health master's programs in Spain shows a certain homogeneity regarding their academic characteristics, but a great heterogeneity in relation to their contents.