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Fly ash (FA), obtained as waste materials from industrial power plants, is generated in large quantities and low recycling. In this study, re-generation of waste FA as cost-effective materials with adsorbent and antibacterial applications was assessed. Alkaline/zinc-activated fly ash nanocomposite (A-FA/Zn) was prepared using one-pot hydrothermal technique. Those nanocomposites are characterized by high surface area and negatively surface charge, which are important influences contributing to an enhancement in adsorption capacity via increase in the number of adsorptive sites and electrostatic interaction between dye molecules-nanocomposites. Additionally, the presence of Zn ions in the prepared nanocomposites represents a key advantage with respect to enhancing antibacterial activity. The feasibility of further enhancing adsorption and antibacterial mechanisms was also examined. It is anticipated that the findings of this study will provide useful information with respect to the development of simple, eco-friendly and low-cost A-FA/Zn with multifunctional applications as organic dye removal and antibacterial purposes.This study aimed to isolate psychrotrophic cellulose-degrading fungi and to investigate their application potential for composting in cold climate regions in China. One out of five psychrotrophic cellulose-degrading fungal isolates was identified as a novel fungal species, Aureobasidium paleasum sp. nov., with a strong straw degradation potential. Enzyme activity assays and FITR spectroscopy revealed high cellulolytic activities of this psychrotrophic fungus at lower temperatures, with high thermal adaptability from 5 °C to 50 °C (optimum at 10 °C). A. paleasum efficiently decomposed rice straws and cellulose at 10 °C compared to the common cellulose-degrading fungus Penicillium oxalicum. In comparison to P. oxalicum, A. paleasum shortened the thermophilic stage, enhanced compost maturity and improved compost quality. Our work suggests that the psychrotrophic fungus A. paleasum is efficient for rice straw degradation and composting at low temperatures, highlighting its application potential for composting in colder regions.Shrub landscaping waste, derived from afforestation of city, has increased annually, making it a promising feedstock for energy production. In this work, the photo-fermentation bio-hydrogen production potential from shrub landscaping waste was evaluated. Eight kinds of shrub landscaping wastes (Photinia fraseri, Buxus megistophylla, Buxus sinica, Pittosporum tobira, Sabina Chinensis, Berberis thunbergii, Ligustrum vicaryi and Ligustrum quihoui) were selected as substrate and the photo-fermentation bio-hydrogen production process of which was optimized. Buxus megistophylla was found to be the most suitable substrate for photo-fermentation bio-hydrogen production. Moreover, the initial pH value, temperature and substrate concentration had significant influence on photo-fermentation bio-hydrogen production. The maximum cumulated hydrogen yield of Buxus megistophylla was 73.82 ± 0.06 mL/g TS under the optimal conditions of light intensity of 3000 Lux, substrate mass concentration of 21.49 g/L, temperature of 29.78 °C, inoculant amount of 25% and initial pH value of 6.78.Pretreatment of lignocellulose materials prior to biogas production is required to minimize biomass recalcitrance and increase biomass digestibility. In this study, the effects of particle size reduction, hydration, and thermal-assisted hydration on Napier grass and silage for methane production were evaluated. Compared to the 4.75-mm particle size Napier grass and silage, 0.425-mm Napier grass and silage showed 72% and 46% increases in methane yield, respectively, whereas hydration pretreatment using hydrogenic effluent increased the methane yields from Napier grass and silage by 23% and 56%, respectively. Superior effects were observed when Napier grass and silage were pretreated with thermal-assisted hydration using hydrogenic effluent for 60 and 15 min, respectively, resulting in methane yields of 385 and 331 mL CH4/g substrateadded. The results indicate that size reduction accompanied by thermal-assisted hydration using hydrogenic effluent as a hydration medium significantly improved the biodegradability of Napier grass and silage.Using mixed microbial consortium (MMC) to accumulate polyhydroxyalkanoate (PHA) is an effective strategy to solve high production cost and reduce the amount of excess sludge. In this study, a process for the production of short-chain-length and medium-chain-length PHA using volatile fatty acids (VFAs) from pretreated wood hydrolysate synergistic with octanoate as co-substrate was proposed. The effects of co-substrate ratios on PHA accumulation ability and physical properties were investigated. The incorporation of co-substrate accelerated the time of PHA and 3-hydroxyoctanoate reaching the maximum production (1834 and 280 mg COD/L). The highest PHA content was 53.0% (w/w), which was equivalent to that reported previously. The biopolymer films possessed high tensile strength, Young's modulus, and could be used in the field of water vapor barrier requirements. The accumulation strategy applied for converting fermentation products VFAs and octanoate co-substrate into high value and yield PHA could potentially demonstrate the valuable for low-cost large-scale production.This study analyzed bacterial community structure for large-scale brewery wastewater treatment at different heights in internal circulation (IC) reactor. Proteobacteria, Bacteroidetes and Chloroflexi were dominant bacteria, which accounted for 64.17%, 64.04%, 59.87% and 55.79% in phylum level, respectively. The unidentified bacteria were accounted for a large proportion in genus level, available data showed that Longilinea, Desulfomicrobium, Caldithrix, Geobacter and Syntrophorhabdus were relatively abundant. Organic fermentation, hydrolysis, and acidification were mainly completed at the bottom, and production of hydrogen and methane were completed in the upper and middle part of reactor. Alpha diversity and cluster distance analysis showed the bacterial community could be divided into bottom, middle and upper part of IC reactor. The IC reactor possessed the CODCr removal efficiency of 80% - 84.09%, and BOD5 of 77.50% - 86% for brewery wastewater. MTP-131 cell line This study would provide bacterial analysis references of IC reactor for industrial wastewater treatment in future.