Evansjeppesen1163
Polyvinylpyrrolidone (PVP) is a chemical material used in intracytoplasmic sperm injection (ICSI) program. The aim of this study was to investigate the ideal time that sperm can be safely incubated in PVP with less structure and DNA damage.
Thirty-one Oligoasthenoteratospermia (OAT) samples were used. Sperm samples were prepared by discontinuous density-gradients method and incubated in 10% PVP at different time intervals (0, 5, 10, 15, 20, and 30 min). The effect of PVP was assessed on sperm DNA fragmentation and viability via SCD assay and Eosin-nigrosin staining respectively.
Data showed there was a significant increase in sperm DNA fragmentation at 10 min compared to 0 min. The viability rate also significantly reduced at 10 min compared to 0 min.
As a result, sperm samples could be incubated with PVP for less than 10 min. While prolonged incubation may significantly damage the sperm DNA integrity and viability.
As a result, sperm samples could be incubated with PVP for less than 10 min. While prolonged incubation may significantly damage the sperm DNA integrity and viability.
The prevalence of erectile dysfunction in men is increasing. As well, the prevalence of diabetes, as one of the causes of sexual dysfunction, is rising in many countries. learn more Due to the failure of common therapies in some patients with sexual dysfunction, it is necessary to develop an effective alternative treatment, such as stem cell therapy, for this problem.
In this randomized single-blinded clinical trial, 20 diabetic patients with erectile dysfunction, who were resistant to common treatments, were selected and divided into two groups of intervention and control (n=10 per group). Autologous mesenchymal stem cells (MSCs) were extracted from oral mucosa and then infused via intracavernosal injection (50-60 106 cells) to the participants of the intervention group. Normal saline was injected in the control group. The patients were followed up with the International Index of Erectile Function (IIEF5) questionnaire, as well as color Doppler duplex ultrasound. Peak systolic velocity (PSV), end diastolic velocity (EDV), and resistance index (RI) were determined at three and six months after the interventions.
The mean IIEF5 scores in the intervention group were 7.2 ± 2.1, 9.2 ± 3.4, and 10.6 ± 4.7 before, three months, and six months after the injection, respectively, showing a significant ascending trend (p value = 0.01). Comparing the intervention and control groups, there was a significant difference in the IIEF5 score change during six months after the injection (p value = 0.02). Regarding the PSV and RI of penis vessels, there were no statistically significant differences between the two groups. However, these parameters showed upward and improving trends in the intervention group.
Intracavernosal injection of stem cells improved sexual function and PSV and RI indices of penile arteries in diabetic patients.
Intracavernosal injection of stem cells improved sexual function and PSV and RI indices of penile arteries in diabetic patients.
Unilateral diaphragmatic paralysis or paresis (UDP) in adults is an often overlooked disease which relevantly impairs the patient's lung function and quality of life. Particularly in idiopathic UDP, there is no evidence for conservative therapy and only little evidence for surgical therapy.
The method involves retrospective single-center analysis of patients with UDP persistent for at least 1 year who were operated by diaphragmatic resection, plication, and augmentation with a polypropylene mesh. The patients were tested for lung and diaphragmatic function, six-minute walk test (6MWT), and blood gas analysis before, 3 and 12 months after surgery.
In total, 85 patients received surgery for UDP. The most frequent reasons for UDP were idiopathic (67%), iatrogenic (mainly cardiac and cervical spine surgery; 24%), and trauma (9%). The mean operation time was 84 ± 24 minutes, the length of hospital stay 8.4 ± 3.9 days, chest tubes were removed after 11.7 ± 4.1 days. Overall morbidity was 42%, mortality 0%. Forced expiratory volume in one second (FEV
) in supine position improved by 12.4% absolute, vital capacity by 11.8% absolute, and sniff nasal inspiratory pressure by 1.4 kPa 12 months after surgery (
<0.001 each). Total lung capacity increased by 6.8% absolute at 12 months (
= 0.001) The 6MWT distance improved by 45.9 m at 3 months and 50.9 m at 12 months (
= 0.001, each).
Surgical therapy for UDP is highly effective in the long term. The superiority over conservative treatments needs to be evaluated prospectively with standardized physiotherapeutic protocols. FEV
in supine position and 6MWT are easy to perform tests and represent statistically and patient-relevant outcomes.
Surgical therapy for UDP is highly effective in the long term. The superiority over conservative treatments needs to be evaluated prospectively with standardized physiotherapeutic protocols. FEV1 in supine position and 6MWT are easy to perform tests and represent statistically and patient-relevant outcomes.The genus Sideritis (Lamiaceae) comprises around 150 species, of which many are popular herbal remedies in Mediterranean folk medicine. Already mentioned by Dioscorides and Theophrastus, the "ironwort" or "Greek mountain tea" has been receiving increased attention in recent years. link2 A European Union herbal monograph and assessment report (HMPC) has been issued, covering the species Sideritis scardica, S. clandestina, S. raeseri, and S. syriaca. This study presents results of a first pharmacognostic examination of the botanical and phytochemical differences among and between these emerging commercial species, and other, less studied species. An HPTLC method is proposed for normal phase separation of the species; this means applying two mobile phases on silica plates and subsequent derivatization with natural product reagent (NP/PEG) for visualization of phenolic compounds and anisaldehyde for a broader detection. With the help of selected reference compounds, a system suitability test was established for proper chromatographic separation. The method was applied to specimens from botanical gardens and commercial raw material in order to test its suitability for differentiation and authentication. The HPTLC analysis also includes, for the first time, S. hyssopifolia and other less used Sideritis species. The results might enable the development of a validated phytochemical fingerprint authentication procedure for quality assurance of Sideritis herba.The etiology of many neurological diseases affecting the central nervous system (CNS) is unknown and still needs more effective and specific therapeutic approaches. link3 Gene therapy has a promising future in treating neurodegenerative disorders by correcting the genetic defects or by therapeutic protein delivery and is now an attraction for neurologists to treat brain disorders, like Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, spinal muscular atrophy, spinocerebellar ataxia, epilepsy, Huntington's disease, stroke, and spinal cord injury. Gene therapy allows the transgene induction, with a unique expression in cells' substrate. This article mainly focuses on the delivering modes of genetic materials in the CNS, which includes viral and non-viral vectors and their application in gene therapy. Despite the many clinical trials conducted so far, data have shown disappointing outcomes. The efforts done to improve outcomes, efficacy, and safety in the identification of targets in various neurological disorders are also discussed here. Adapting gene therapy as a new therapeutic approach for treating neurological disorders seems to be promising, with early detection and delivery of therapy before the neuron is lost, helping a lot the development of new therapeutic options to translate to the clinic.In 2020, updated versions of the clinical practice guidelines of the European Association for Endoscopic Surgery, the Canadian Adult Obesity Clinical Practice Guidelines and the Dutch Federation for Medical Specialist clinical practice guidelines on bariatric surgery were published. We systematically reviewed and compared them on recommendations and references. Although the authors would have had access to the same literature, only 5 out of 655 unique references were used by all 3 guidelines and just 49 references by any combination of 2 guidelines. These findings attest to the subjectivity involved in clinical practice guidelines development and could be the cause for the observed differences in recommendations. International cooperation in guideline development might be a conceivable solution.Lack of standardization in the Roux-en-Y gastric bypass (RY-GBP) is quite well established. We all learned the basics of the technique, but a lot of differences do exist in performing each step of the procedure. Based on scientific evidences, coming from an extensive and meticulous review of the literature of the last 20 years, we thus address the different technical steps of the procedure and their importance to try and propose a standardization of RYGBP. A lot of possibilities exist at each and every step of a RYGBP. They influence the postoperative complications, the end weight loss (EWL), weight regain, and resolution of obesity bounded comorbidities. Furthermore, lack of standardization leads to problems regarding comparison of scientific data in the related literature.The automatic localization of the lumbar region is essential for the diagnosis of lumbar diseases, the study of lumbar morphology, and the surgical planning. Although the existing researches have made great progress, it still faces several challenges. First, the various lumbar diseases and pathologies cause different abnormalities in the lumbar shape and appearance. Second, the numbers of lumbar vertebrae are irregular (some people have an additional vertebra L6). To tackle these challenges, we propose a novel lumbar region localization method based on bone anatomy feature graphs. Specifically, a feature graph (called LS) considering the anatomy of the sacrum and the lumbar vertebra is proposed to locate the inferior boundary of L5 or L6. A feature graph (called TL) considering the anatomy of the thoracic vertebra and the lumbar vertebra is proposed to locate the superior boundary of L1. Extensive experimental analysis is performed on a public available dataset xVertSeg and a private dataset which contains 197 CT scans. The localization results show that the proposed method is robust and can be applied to normal scans, scoliosis scans, deformity scans, hyperosteogeny scans, 6 lumbar vertebrae scans and lumbar implant scans. The Dice and Jaccard coefficients are 98.09 ± 0.84% and 96.27 ± 1.62% respectively. Graphical Abstract Lumbar Region Localization Framework.
The accuracy of the CyberKnife Synchrony Respiratory Tracking System is dependent on the breathing pattern of a patient. Therefore, the tracking error in each patient must be determined. Support vector regression (SVR) can be used to easily identify the tracking error in each patient. This study aimed to develop a system with SVR that can predict tracking error according to a patient's respiratory waveform.
Datasets of the respiratory waveforms of 93 patients were obtained. The feature variables were variation in respiration amplitude, tumor velocity, and phase shift between tumor and the chest wall, and the target variable was tracking error. A learning model was evaluated with tenfold cross-validation. We documented the difference between the predicted and actual tracking errors and assessed the correlation coefficient and coefficient of determination.
The average difference and maximum difference between the actual and predicted tracking errors were 0.57 ± 0.63 mm and 2.1 mm, respectively. The correlation coefficient and coefficient of determination were 0.
