Ernstsencamp2016

9990. Enrichment factors of the method were obtained from 2045 to 3080. The limits of detection (LODs, S/N = 3) and limits of quantification (LOQs, S/N = 10) were calculated as 0.0031-0.0111 ng/mL and 0.01-0.05 ng/mL, respectively. The precisions of intra-day and inter-day were obtained with the relative standard deviations (RSDs) at 1.5-6.6% and 0.8-6.9%, respectively. The fiber-to-fiber producibility was achieved with RSDs ranged from 3.5% to 11.4%. The method was applied to detect PCBs in river water and soil samples. The contents were calculated at 0.040-0.147 ng/mL in water and 0.520-3.218 ng/g in soil. The C3N5 as SPME fiber coating material may be applied to extract and preconcentrate other environmental pollutants which have similar chemical structures with PCBs.A novel polyhedral oligomeric siloxane (POSS)-based zwitterionic monolithic capillary column was prepared via one-pot polymerization in ionic liquid porogen, using N,N-dimethyl-N-methacryloxyethyl-N-(3-sulfopropyl)ammonium betaine (DMMSA) and methacrylic ethyl trimethylammonium chloride (META) as binary functional monomers, and methacryl substituted POSS as cross-linker. The pore structure, permeability and homogeneity were well tuned by optimizing the polymerization conditions. The resultant monolith was characterized by scanning electron microscopy, nitrogen adsorption/desorption isotherm and Fourier transform infrared spectroscopy. The incorporation of zwitterionic ligand (DMMSA), quaternary amine group (META) and rigid POSS skeleton endows the hybrid organic-silica monolith with high hydrophilicity, electrostatic interaction and good mechanical stability, as well as a tunable electroosmotic flow over wide pH range. A close investigation of capillary electrochromatographic separations of different types of polar compounds such as bases, nucleosides and benzoic acids on such stationary phase exhibited a retention independent column efficiency up to 118,000 plates/m (thiourea), as well as a mixed-mode hydrophilic interaction chromatography (HILIC) retention mechanism including weak electrostatic interaction, hydrophobic interaction and anion exchange.An integrated QuEChERS strategy was developed by combining the extraction and purification processes into a single step. All of the pretreatment procedures could be performed in one tube within 5 min with the aid of magnetic nanoparticles and careful optimization of the key parameters, including the dosages of the sorbents (magnetic nanoparticles, C18, and graphitized carbon black), dehydrating and salting out reagents. The optimal method was validated and compared with the conventional QuEChERS method, demonstrating its clear superiority in terms of operating procedure, sample pretreatment time, and reagent dosages while affording equivalent pesticide recoveries and matrix effects. Further application of this method was performed to analyze 127 pesticide residues in solanaceous vegetables (tomato, pepper, and eggplant), leafy vegetables (brassica campestris and cabbage), legumes (green beans and cowpea), melon-type vegetables (cucumber and towel gourd), and a root vegetable (water bamboo), with the mean recoveries of the pesticides in the individual vegetable samples ranging from 70.6 to 92.8%. The method LOQs for these pesticides ranged from 10 to 50 μg/kg depending on the matrix. These results fully confirmed its wide applicability and versatility for achieving robust, rapid, and high-throughput multi-pesticide residues analysis in vegetable samples. More importantly, the developed strategy provides a greener and more "QuEChERS" design concept, which could be applied to the analysis of numerous types of pesticide residues in various matrices.The developmental pattern of the amygdala throughout childhood and adolescence has been inconsistently reported in previous neuroimaging studies. Given the relatively small size of the amygdala on full brain MRI scans, discrepancies may be partly due to methodological differences in amygdalar segmentation. To investigate the impact of volume extraction methods on amygdala volume, we compared FreeSurfer, FSL and volBrain segmentation measurements with those obtained by manual tracing. The manual tracing method, which we used as the 'gold standard', exhibited almost perfect intra- and inter-rater reliability. We observed systematic differences in amygdala volumes between automatic (FreeSurfer and volBrain) and manual methods. Specifically, compared with the manual tracing, FreeSurfer estimated larger amygdalae, and volBrain produced smaller amygdalae while FSL demonstrated a mixed pattern. The tracing bias was not uniform, but higher for smaller amygdalae. We further modeled amygdalar growth curves using accelee warranted for current automatic tools to achieve more accurate amygdala structure at school age, calling for next generation tools.Conditioning can drastically improve the dewaterability of sewage sludge and is widely practiced in most wastewater treatment plants (WWTPs). Sludge conditioning was also reported as a crucial step in sludge treatment to attenuate antibiotic resistance, but it remains unclear whether the attenuated antibiotic resistance by conditioning treatments would guarantee low abundance of antibiotic resistance genes (ARGs) in the compost products of municipal sewage sludge. Herein, the impacts of three conditioning treatments, including bioleaching and chemical conditioning using Fe[III]/CaO or polyacrylamide (PAM), on the abundances of 20 ARGs and 4 mobile genetic elements (MGEs) during conventional aerobic composting of dewatered sludge were investigated. It was found that the absolute and relative abundances of total ARGs in compost product of bioleached sludge accounted for only 13.8%-28.8% of that in compost products of un-conditioned, Fe[III]/CaO-conditioned, or PAM-conditioned sludges. Besides, bioleaching conditioning resulted in the lowest abundances of ARG subtypes and ARG-associated bacteria in the sludge compost product. The shift of ARG profiles in the bioleached sludge composting can be mainly ascribed to the ARG-associated bacteria, while the MGEs drove the ARG profiles during conventional composting of un-conditioned sludge and the two chemically conditioned sludge. Rapamycin solubility dmso Thus, bioleaching conditioning is superior to the chemical conditioning using Fe[III]/CaO or PAM in mitigating antibiotic resistance in sludge compost products, which was contributed by the pre-removal of ARGs prior to composting treatment and the potential limitation of ARGs transfer during conventional composting.In this work, the use of biomethane produced from local biogas plants is proposed as renewable fuel for light marine transport. A profitability analysis is performed for three real biogas production plants located in Cornwall (United Kingdom), considering a total of 66 different scenarios where critical parameters such as distance from production point to gas grid, subsidies, etcetera, were evaluated. Even though the idea is promising to decarbonize the marine transport sector, under the current conditions, the approach is not profitable. The results show that profitability depends on the size of the biogas plant. The largest biogas plant studied can be profitable if feed-in tariffs subsidies between 36.6 and 45.7 €/MWh are reached, while for the smallest plant, subsidies should range between 65 and 82.7 €/MWh. The tax to be paid per ton of CO2 emitted by the shipping owner, was also examined given its impact in this green route profitability. Values seven times greater than current taxes are needed to reach profitability, revealing the lack of competitiveness of renewable fuels vs traditional fuels in this application. Subsidies to make up a percentage of the investment are also proposed, revealing that even at 100% of investment subsidized, this green approach is still not profitable. The results highlight the need for further ambitious political actions in the pursuit of sustainable societies.In order to achieve thermal treatment of coal slime and depth control of NOx, a 75 t/h circulating fluidized bed (CFB) boiler arranging post-combustion air is constructed. In the experiment, the combustion atmosphere and the ratio of primary air and secondary air are adjusted to decrease the NOx emissions below 50 mg/m3 (dry basis at 6% O2). Compared with conventional CFB combustion, the post-combustion technology decreases the NOx emissions from 92.9 to 65.4 mg/m3 by adjusting the combustion atmosphere. Then, the primary air volume is adjusted to decrease the NOx emissions further. On the one hand, decreasing primary air volume contributes to inhibiting the NOx generation in the dense phase. On the other hand, it is proved that the combination of the cyclone and a post-combustion chamber plays a crucial role in the de-NOx process of post-combustion technology. More char particles are brought to the cyclone as the primary air volume decreases. The NOx reduction in the cyclone and the post-combustion chamber is promoted. Finally, the NOx emissions are decreased to 42.6 mg/m3 when the ratio of primary air and secondary air is 50.0%. In addition, the SO2 emissions and the combustion efficiency during the ultra-low NOx condition are 23.2 mg/m3 and 98.3%, respectively.The reducing gases produced and NO reduction by sewage sludge combustion were investigated in a self-made cement precalciner. The dual role of O2 concentration (0-5 vol%) in the production characteristics of reducing gases and the reduction efficiency of NO were evaluated experimentally. TG-FTIR analysis demonstrated that the key reducing gaseous species produced by sewage sludge combustion were HCN, NH3, CO, and CH4. And experiments demonstrated that O2 concentration had pronounced effects on NH3 distribution, the maximum production rate was obtained at an O2 concentration of 3 vol%. Meanwhile, the reducing gases NH3 and CO were the key species for NO reduction in the cement precalciner, and the reduction efficiency of NO, when reduced by NH3, increased with an increase in O2 concentration, while the reduction performance of NO by CO was limited by O2 concentration. Therefore, O2 concentration greatly influences NO reduction efficiency by sewage sludge combustion; the maximum NO reduction efficiency was 61.67% at an O2 concentration of 3 vol%. The difference in NO reduction by sewage sludge combustion under different O2 concentrations was primarily attributed to NH3 production rate and NO reduction by NH3 and CO, which is greatly affected by O2 concentration. Sewage sludge combustion can result in NO reduction in the cement kiln flue gas and resource utilisation of sewage sludge.

Shikonin is one of the major phytochemical components of Lithospermum erythrorhizon (Purple Cromwell), which is a type of medicinal herb broadly utilized in traditional Chinese medicine. It is well established that shikonin possesses remarkable therapeutic actions on various diseases, with the underlying mechanisms, pharmacokinetics and toxicological effects elusive. Also, the clinical trial and pharmaceutical study of shikonin remain to be comprehensively delineated.

The present review aimed to systematically summarize the updated knowledge regarding the therapeutic actions, pharmacokinetics, toxicological effects, clinical trial and pharmaceutical study of shikonin.

The information contained in this review article were retrieved from some authoritative databases including Web of Science, PubMed, Google scholar, Chinese National Knowledge Infrastructure (CNKI), Wanfang Database and so on, till August 2021.

Shikonin exerts multiple therapeutic efficacies, such as anti-inflammation, anti-cancer, cardiovascular protection, anti-microbiomes, analgesia, anti-obesity, brain protection, and so on, mainly by regulating the NF-κB, PI3K/Akt/MAPKs, Akt/mTOR, TGF-β, GSK3β, TLR4/Akt signaling pathways, NLRP3 inflammasome, reactive oxygen stress, Bax/Bcl-2, etc.