Erichsensilva2304

The outbreak of coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has posed a serious threat to global public health. The mechanism of pathogenesis and the host immune response to SARS-CoV-2 infection are largely unknown. In the present study, we applied a quantitative proteomic technology to identify and quantify the ubiquitination changes that occur in both the virus and the Vero E6 cells during SARS-CoV-2 infection. By applying label-free, quantitative liquid chromatography with tandem mass spectrometry proteomics, 8943 lysine ubiquitination sites on 3086 proteins were identified, of which 138 sites on 104 proteins were quantified as significantly upregulated, while 828 sites on 447 proteins were downregulated at 72 h post-infection. selleckchem Bioinformatics analysis suggested that SARS-CoV-2 infection might modulate host immune responses through the ubiquitination of important proteins, including USP5, IQGAP1, TRIM28, and Hsp90. Ubiquitination modification was also observed on 11 SAR-CoV-2 proteins, including proteins involved in virus replication and inhibition of the host innate immune response. Our study provides new insights into the interaction between SARS-CoV-2 and the host as well as potential targets for the prevention and treatment of COVID-19.Transcriptional regulatory protein (TRP)-based whole-cell biosensors are widely used nowadays. link2 Here, they were demonstrated to have great potential application in screening cell efflux and influx pumps for small molecules. First, a vanillin whole-cell biosensor was developed by altering the specificity of a TRP, VanR, and strains with improved vanillin productions that were selected from a random genome mutagenesis library by using this biosensor as a high-throughput screening tool. A high intracellular vanillin concentration was found to accumulate due to the inactivation of the AcrA protein, indicating the involvement of this protein in vanillin efflux. Then, the application of this biosensor was extended to explore efflux and influx pumps, combined with directed genome evolution. Elevated intracellular vanillin levels resulting from efflux pump inactivation or influx pump overexpression could be rapidly detected by the whole-cell biosensor, markedly facilitating the identification of genome targets related to small-molecule transmembrane transport, which is of great importance in metabolic engineering.Chiral stirred optical and magnetic properties, through the doping of assembled ultrasmall metal clusters (AMCs), are promising discernment to rivet the molecule-like quantum devices. Here, the single manganese (Mn) atom doping and assembly of the gold cluster (Au8), leading to the chirality driven magnetism, has been achieved through a ligand-mediated growth. link3 The X-ray absorption near edge structure and electron paramagnetic resonance studies corroborate the tetrahedral coordinated local structure of Mn dopant in the Au host. The optical and vibrational circular dichroic analysis affirms the modulation of chirality (negative to positive) in the presence of the Mn. A distinct ferromagnetic hysteresis loop at 300 K shows Mn ridden chiral sensitive ferromagnetism in contrary to the ligand influenced superparamagnetic undoped AMCs. The spin-polarized density functional theory level of calculations reveal the partial overlapping of spin-up and -down density of states in the doped AMCs, attributing to the ferromagnetic nature as like a molecular magnet suitable for the opto-spintronics application.As energy-conversion materials, organic-inorganic hybrid perovskites remain a research- and finance-intensive topic. However, even for the arguably most iconic representatives, methylammonium and formamidinium lead halides, the crystal structures of several polymorphs have remained undetermined. Herein, we describe the incommensurately modulated structure of MAPbBr3 in (3+1)D superspace, as deduced from single-crystal X-ray diffractometry despite systematic twinning. Affirming the published average space group, we determined the superspace group Imma(00γ)s00 with cell parameters of a = 8.4657(9), b = 11.7303(12), c = 8.2388(8) Å, and q = 0.2022(8)c*. Via group-subgroup and mode analyses using irreducible representations, we establish symmetry relationships to the well-known cubic and orthorhombic polymorphs and break down distortions into the average tilt system a-b0a- and modulated contributions to tilt and deformation of the PbBr6 coordination polyhedra. Not only does our model fill a long-standing gap in structural knowledge, but it may also serve as a starting point for elucidating other modulated structures within this substance class.

Bone parameters are influenced by multiple factors. However, when comparing sports, few studies have considered the simultaneous interference of these factors in bone parameters.

To compare bone mineral density (BMD) and bone mineral content (BMC) between university athletes from different sports, according to sex.

Cross-sectional study with 71 male (M) and female (F) university athletes practicing indoor soccer (M = 14, F = 20), judo (M = 8, F = 6) and volleyball (M = 11, F = 12) aged 22.37 (± 3.71) years. Dependent variables were BMD and BMC corrected by height measured by dual energy X-ray absorptiometry. The sport practiced (indoor soccer, judo and volleyball) was the independent variable and covariates were fat mass, lean tissue mass, training volume, practice time and gynecological aspects (in females athletes) such as regular menstruation and use of oral contraceptives. Analysis of covariance was used, with p <0.05.

Female volleyball players [mean 1.23; Standard Error (SE) 0.03] presented higher lumbar BMD values compared to judo athletes (mean 1.08; SE 0.05). In addition, lumbar BMC values of indoor soccer athletes (mean 0.41; SE 0.01) was higher compared to judo athletes (mean 0.34; SE 0.02). In males, no differences were observed among sports.

Sports performance technicians and professionals will be able to broaden the understanding of variations in BMD and BMC lumbar between judo, indoor soccer and volleyball athletes, which allows monitoring these parameters in the sports context and in the athlete's health.

Sports performance technicians and professionals will be able to broaden the understanding of variations in BMD and BMC lumbar between judo, indoor soccer and volleyball athletes, which allows monitoring these parameters in the sports context and in the athlete's health.Social detachment to prevent the spread of the COVID-19 pandemics in the year 2020 can significantly contribute to the physical inactivity of citizens worldwide. The study aimed to analyze the level of physical activity by identifying the training methods used during the social isolation resulting from the COVID-19 pandemic. Sixty-eight Brazilian athletes (both sex, 14.7 ± 1.68 years) answered and adapted the International Physical Activity Questionnaire (I-PAQ) through an online platform. Participants were asked to report their level of physical activity before and during the period of social distance. 67.7% of the interviewed athletes said they were able to adapt their sports training to the isolation environment under the guidance of a distance physical education professional. Only 4.38% of the sample was not training under such supervision and, therefore, inactive. Among the activities performed in the isolation environment, calisthenics was the primary practice (Effect Size ƒ2 = 0.50, p less then 0.0001) and the sport practiced at home was the secondary practice (Effect Size ƒ2 = 0.27, p = 0.004). During the pandemic, the training hours of athletes reduced significantly from ~ 3h to ~ 1h per day (Effect size 1.74, p less then 0.0001), as well as, the perceived intensity decreased from "high" to "moderate" (Effect size 1.38, p less then 0.0001). The weekly training frequency decreased from ~ 6 to 7 days to ~ 3 to 5 days (Effect size 0.40, p = 0.03). Therefore, despite the social distance and the reduced pace of training, the young Brazilian athletes analyzed managed to remain physically active during the COVID-19 pandemic.

Endurance sports are strongly associated with maximum oxygen uptake, anaerobic threshold, running economy and body fat percentage. Despite the importance for performance of the low-fat mass being a consensus in the literature, there are no data about the importance of the pattern of fat distribution. Therefore, the aim of the present study was to investigate the association between fat mass distribution with triathlon performance and physiological determinants of performance maximal oxygen consumption (VO2max), ventilatory threshold (AT) and running economy (RE), and to verify the predictive value for performance of gynoid or android fat mass distribution.

Thirty-nine triathletes (38.8±6.9 years, 174.8±6.5cm and 74.3±8.8kg) were evaluated for anthropometric (total body mass, fat mass, lean mass, android and gynoid fat mass) and physiological (VO2max, AT and RE) parameters. Split and overall race times were registered.

Overall race time relationship with gynoid fat mass (r=.529, p<.05) was classified as moderate higher than and with android fat mass (r=.416, p<.05) was classified as low. All split times and overall race time presented significant positive correlation with only total fat mass (%) (r =.329 to .574, p<.05) and with gynoid fat mass (%) (r=.359 to .529, p<.05). Overall race time can be better predicted by gynoid fat mass (ß=0.529, t=4.093, p<0.001, r2=0.28) than by android fat mass (ß =0.416, t=2.997, p=0.005, r2=0.17).

Fat mass distribution is associated with triathlon performance, and the gynoid fat pattern is worse for triathlon performance than the android pattern.

Fat mass distribution is associated with triathlon performance, and the gynoid fat pattern is worse for triathlon performance than the android pattern.In the last few years, some inherited determinants have been associated with elite athletic performance, but its polygenic trait character has limited the correct definition of elite athlete's genomic profile. This qualitative descriptive study aims to summarise the current understanding about genetic and epigenetic factors in elite athletes, as well as their genomic profile in association with sport-type, sex, ethnicity, psychological traits and sport injuries. A narrative review of the literature across a broad cross-section of the elite athletes' genomic profile was undertaken. Elite performance relies on rare gene variants within a great interface between molecular, cellular and behavioural sport-related phenotypes and the environment, which is still poorly understood. ACTN3 rs1815739 and ACE I/D polymorphisms appear to be associated to specific sprint phenotypes and influence the athletic status, i.e., the rs1815739 variant is more influential to 200-m performance and the ACE ID polymorphism is more involved in the longer, 400-m sprint performance. Generally, athletes show endurance-based sports characteristics or power-based sports characteristics, but some studies have reported some genes associations to both sports-based characteristics. Furthermore, genetic studies with larger cohorts of single-sport athletes might be preferable than studies combining athletes of different sports, given the existence of distinct athlete profiles and sport demands. Athletic performance may be influenced by the serotonergic pathway and the potential injury risk (namely stress fracture) might be associated to a genetic predisposition associated to the mechanical loading from the intense physical exercise. The study of gene variants associated to sex and ethnicity-related to athletic performance needs further investigation. The combination of genome-wide association studies addressing the genetic architecture of athletes and the subsequent replication and validation studies might for additional genetic data is mandatory.