Emborgbentsen0020
Calcium-activated potassium channels (KCa) are a specific type of potassium channel activated by intracellular calcium concentration changes. This group of potassium channels plays fundamental roles ranging from regulating neuronal excitability to immune cell activation. Many human diseases such as schizophrenia, hypertension, epilepsy, and cancers have been linked to mutations in this group of potassium channels. Although the KCa channels have been extensively studied electrophysiologically and pharmacologically, their spatiotemporal gene expression during embryogenesis remains mostly unknown.
Using zebrafish as a model, we identified and renamed 14 KCa genes. We further performed phylogenetic and syntenic analyses on vertebrate KCa genes. Our data revealed that the number of KCa genes in zebrafish was increased, most likely due to teleost-specific whole-genome duplication. Moreover, we examined zebrafish KCa gene expression during early embryogenesis. The duplicated ohnologous genes show distinct and overlapped gene expression. Furthermore, we found that zebrafish KCa genes are expressed in various tissues and organs (somites, fins, olfactory regions, eye, kidney, and so on) and neuronal tissues, suggesting that they may play important roles during zebrafish embryogenesis.
Our phylogenetic and developmental analyses shed light on the potential functions of the KCa genes during embryogenesis related to congenital diseases and human channelopathies.
Our phylogenetic and developmental analyses shed light on the potential functions of the KCa genes during embryogenesis related to congenital diseases and human channelopathies.
Severe acute respiratory syndrome coronavirus 2 is a respiratory virus that poses risks to the nutrition status and survival of infected patients, yet there is paucity of data to inform evidence-based quality care.
We collected data on the nutrition care provided to patients with coronavirus disease 2019 (COVID-19) by registered dietitian nutritionists (RDNs).
Hospitalized COVID-19 patients (N = 101) in this cohort were older adults and had elevated body mass index. The most frequent nutrition problems were inadequate oral intake (46.7%), inadequate energy intake (18.9%), and malnutrition (18.4%). These problems were managed predominantly with enteral nutrition, food supplements, and multivitamin-multimineral supplement therapy. Over 90% of documented problems required a follow-up.
This data set is the first of its kind to report on the types of nutrition diagnoses and interventions for COVID-19 cases used by RDNs and highlights the need for increased and continued nutrition care.
This data set is the first of its kind to report on the types of nutrition diagnoses and interventions for COVID-19 cases used by RDNs and highlights the need for increased and continued nutrition care.
Complete decomposition of silicate rock matrices is crucial in determining their isotopic compositions, but acid dissolution in a high-pressure steel-jacketed bomb, which has been the only powerful, effective technique thus far, is time-consuming and expensive. Rock dissolution using ammonium bifluoride (ABF), as described here, is a viable alternative.
Geological reference materials (GRMs) were digested using ABF in closed Teflon beakers at temperatures of 220/230°C in a convection oven and subsequently treated with HNO
. Hf-Sr-Nd were separated and purified using ion-exchange chemistry columns calibrated for 50-2mg samples. The isotopic compositions of Sr-Nd were measured by Thermal Ionization Mass Spectrometry, while that of Hf by Multi-Collector Inductively Coupled Plasma Mass Spectrometry, both with normal 10
Ω and gain calibrated 10
Ω amplifiers.
Total procedural blanks of our protocol are 0.5ng for Sr, 0.2ng for Nd and <25 pg for Hf. SR-0813 in vivo Test runs with GRMs, ranging in composition from basic to felsic and dissolved in ABF, yield accurate
Sr/
Sr,
Nd/
Nd and
Hf/
Hf isotope ratios as compared with those obtained with the bomb dissolution technique. Reproducibilities were comparable, on the order of 10-20 ppm. Our technique allows combined Hf-Sr-Nd isotope analyses of low-mass (50-2mg) samples.
The ABF digestion is an alternative technique to high-pressure bomb dissolution in matrix decomposition for accurate and reproducible Hf-Nd-Sr isotope analyses of geological samples within a reasonable time (3-4days), with high sample throughput and low costs in geochemistry and environmental sciences.
The ABF digestion is an alternative technique to high-pressure bomb dissolution in matrix decomposition for accurate and reproducible Hf-Nd-Sr isotope analyses of geological samples within a reasonable time (3-4 days), with high sample throughput and low costs in geochemistry and environmental sciences.
Although changes in skin depend on the external environment, researchers have performed only a few studies on effect of the actual environment. Most studies have researched skin characterization based on changes in the humidity or temperature.
This study aimed to evaluate changes in the skin based on the difference in indoor and outdoor temperatures and humidity during summer in South Korea and Southeast Asia.
Twenty-two female participants aged 25-39years were included. Skin hydration, sebum (cheek, forehead), colour, transparency and pores of the participants were measured after a 30-min exposure to high temperature and high humidity (HTHH) environment and a 30-min exposure to low temperature and low humidity (LTLH) environment. Subsequently, exposure to HTHH environment for 30min +LTLH environment for 30min was performed after a total of 1h and repeated.
Repeated exposure to HTHH and LTLH environments increased the skin's sebum content and haemoglobin index. Additionally, skin elasticity was significantly reduced, with patients in their 30s showing greater changes than those in their 20s.
Repeated differences in temperature and humidity cause skin ageing, loosen skin vessels and reduce skin elasticity, thereby leading to skin ageing.
Repeated differences in temperature and humidity cause skin ageing, loosen skin vessels and reduce skin elasticity, thereby leading to skin ageing.
