Ejlersenkline5254

The secondary structure of the TSL protein contained α-helix (51.72%), extended strand (12.20%), β-bridge (4.78%) and random coil (31.30%) in the secondary structure, and a 3D homology model was generated using 5cj9.1.A as a template. Phylogenetic analysis revealed a close genetic relationship in the TSL protein between

and

. In addition, quantitative real-time PCR assay detected the

gene expression in the head, chest, abdomen and foot of

, with the highest expression in the head and low expression in the foot.

The

gene is characterized in

at a genomic level, and the prediction of the TSL protein structure and the elucidation of the tissue-specific

gene expression in

provide a basis for the further studies on the gene functions.

The tsl gene is characterized in An. dirus at a genomic level, and the prediction of the TSL protein structure and the elucidation of the tissue-specific tsl gene expression in An. dirus provide a basis for the further studies on the gene functions.

To investigate the prevalence and risk factors of

infections among AIDS patients in Nanchang City.

A cross-sectional questionnaire survey was conducted among AIDS patients in Nanchang City during the period between May and September, 2016.

infection was detected in patients'stool samples using a PCR assay, and the CD4

T cell count was measured in subjects'blood samples. In addition, the risk factors of

infection in AIDS patients were identified using univariate and multivariate logistic regression analyses.

A survey was conducted in Nanchang City from May to September 2016. A total of 505 AIDS patients were investigated, and the prevalence of

infection was 4.16%. Univariate analysis revealed that

infection correlated with the occupation (



= 8.595,

= 0.049), education level (



= 14.494,

= 0.001), type of daily drinking water (



= 10.750,

= 0.020), root of HIV infections (



= 8.755,

= 0.026) and receiving anti-HIV therapy (



= 23.083,

= 0.001) among AIDS patients, and multivariate logistic regression analysis identified daily direct drinking of tap water as a risk factor of

infections [odds ratio (

) = 7.988, 95% confidential interval (

) (1.160, 55.004)] and anti-HIV therapy as a protective factor of

infection [

= 0.183, 95%

(0.049, 0.685)].

The prevalence of

is 4.16% among AIDS patients in Nanchang City. CWI1-2 Daily direct drinking of tap water is a risk factor, and anti-HIV therapy is a protective factor of

infection among AIDS patients living in Nanchang City.

The prevalence of B. hominis is 4.16% among AIDS patients in Nanchang City. Daily direct drinking of tap water is a risk factor, and anti-HIV therapy is a protective factor of B. hominis infection among AIDS patients living in Nanchang City.

To investigate the disease progression and immunoprotective characteristics in mice re-infected with homogeneous/heterogeneous

strains following cure of

infections with chloroquine at the peak of parasitemia.

C57BL/6 mice were infected with the non-lethal

17XNL strain, and half of mice were given treatment with chloroquine at the peak of parasitemia (9 days post-infection), while the other mice were self-cured naturally. Then, all cured mice were re-infected with the equivalent lethal

17XL or

ANKA strain 90 days following primary

infections. The parasitemia levels during primary infections and reinfections were measured by microscopic examinations of Giemsa-stained thin blood films, and the levels of the IgG antibody in sera and the percentages of memory T cell subsets in spleen cells were detected in mice using ELISA and flow cytometry before and after parasite reinfections, respectively.

Following primary infections with the

17XNL strain, the serum IgG antibody levels were (5.047 ffect the production of effective immune protections in mice during parasite re-infections. Following a primary infection, mice show a protection against re-infections with either homogeneous or heterogeneous Plasmodium strains, and a higher-level resistance to re-infections with homogeneous parasite strains is found than with heterogeneous strains.

To analyze the changing tendency in the endemic situation of schistosomiasis in the national surveillance sites of Hubei Province, so as to provide the scientific evidence for facilitating the progress towards the elimination of schistosomiasis in the province.

According to the

, a total of 65 national schistosomiasis surveillance sites were assigned in Hubei Province, and the

infections in humans, domestic animals, snails and wild feces were monitored in these national surveillance sites from 2015 to 2019. All data pertaining to the surveillance results were descriptively analyzed.

There were 151 159 person-time local residents and 70 928 person-time mobile populations screened for

infections in the 65 national surveillance sites of Hubei Province from 2015 to 2019, and the seroprevalance of

infections reduced from 3.45% (1 077/31 197) in 2015 to 1.57% (431/27 371) in 2019 among local residents and from 1.06% (98/9 249) in 2015 to 0.81% (116/14 318) in 2019 among mobile populations. During tall endemic situation of schistosomiasis appears a tendency towards a decline in Hubei Province during the period from 2015 through 2019; however, the schistosomiasis transmission risk remains. Improving the surveillance system of schistosomiasis and increasing the sensitivity and performance of the surveillance system are required to provide insights into the implementation of precision control strategy and interventions in Hubei Province.

The overall endemic situation of schistosomiasis appears a tendency towards a decline in Hubei Province during the period from 2015 through 2019; however, the schistosomiasis transmission risk remains. Improving the surveillance system of schistosomiasis and increasing the sensitivity and performance of the surveillance system are required to provide insights into the implementation of precision control strategy and interventions in Hubei Province.

To investigate the factors affecting the degradation of niclosamide in the soil, so as to provide the evidence for the assessment of the environmental safety in the field snail control with niclosamide.

A high performance liquid chromatography was established for the determination of niclosamide in the field. Then, the degradation of niclosamide was investigated in soils with different moistures (10%, 30%, 50%, 70% and 90%), temperatures [(15 ± 1), (25 ± 1), (35 ± 1) °C], initial concentrations (1, 5, 10 mg/kg) and in sterilized and non-sterilized soils. In addition, the degradation of niclosamide was fitted with the first-order kinetics equation, and the degradation half-life was calculated.

The niclosamide residues gradually decreased over time in soils with different moistures, and a higher rate of degradation was seen in soils with a higher moisture. The degradation half-life of niclosamide reduced from 4.258 d in the soil with a 10% moisture to 2.412 d in the soil with a 90% moisture. The niclosamide residues gradually decreased over time in soils with different temperatures, and a higher rate of degradation was seen in soils with a higher temperature.