Eatonloft1057

4). These results indicate that silkworm models are a useful tool for identifying anti-MAC antibiotics candidates with veritable therapeutic effects.Since the first report of COVID-19 in December 2019, little is known about therapeutic usage of hydroxychloroquine in pediatric patients with COVID-19. We retrospectively retrieved data for SARS-CoV-2 PCR positive pediatric patients from 20 hospitals in 8 different cities in Turkey. We obtained patients' epidemiological, clinical, laboratory features and drugs used for treatment of COVID-19. 237 nasopharyngeal swab SARS-CoV-2 PCR positive children were included into the study from March 26 to June 20, 2020. The mean age of asymptomatic children (118±62 months) was found to be higher than that of symptomatic children (89±69 months). Symptomatic children had a significantly lower mean lymphocyte count and higher mean CRP, D-dimer value, procalcitonin and LDH than asymptomatic children in univariate analysis. Out of 156 children, 78 (50%) children received Hydroxychloroquine-containing regimen, 15 of them were treated with hydroxychloroquine + azithromycin + oseltamivir, 44 were treated with hydroxychloroquine + azithromycin and 21 were only treated with hydroxychloroquine. Among the 156 patients who received medical treatment, 90 (58%) patients had pre and/or post-treatment ECG performed upon them. However, none of them either reported ECG abnormalities or a need for discontinuation of hydroxychloroquine because of adverse drug reaction.Isolation of seasonal coronaviruses, which include human coronavirus (HCoV) OC43, HCoV-HKU1, and HCoV-NL63, from primary cultures is difficult because it requires experienced handling, an exception being HCoV-229E, which can be isolated using cell lines such as RD-18S and HeLa-ACE2-TMPRSS2. We aimed to isolate seasonal CoVs in Yamagata, Japan to obtain infective virions useful for further research and to accelerate fundamental studies on HCoVs and SARS-CoV-2. Using modified air-liquid interface (ALI) culture of the normal human airway epithelium from earlier studies, we isolated 29 HCoVs (80.6% 16, 6, 6, and 1 isolates of HCoV-OC43, HCoV-HKU1, HCoV-NL63, and HCoV-229E, respectively) from 36 cryopreserved nasopharyngeal specimens. In ALI cultures of HCoV-OC43 and HCoV-NL63, the harvested medium contained more than 1×104 genome copies/µL at every tested time point during the more than 100 days of culture. Four isolates of HCoV-NL63 were further subcultured and successfully propagated in an LLC-MK2 cell line. Our results suggest that ALI culture is useful for isolating seasonal CoVs and sustainably obtaining HCoV-OC43 and HCoV-NL63 virions. Furthermore, the LLC-MK2 cell line in combination with ALI cultures can be used for the large-scale culturing of HCoV-NL63. Further investigations are necessary to develop methods for culturing difficult-to-culture seasonal CoVs in cell lines.Human papillomavirus (HPV) infection is one of the most common sexually transmitted infections. Persistent infection is associated with the development of cervical cancer. Men could serve as a medium for HPV transmission among women, and little is known about the prevalence and genotypes of HPV infection among men in Yunnan province. This study is designed for investigating these parameters among men in this region. Clinical information was collected from January 2015 to May 2020, and a total of 369 men who sought to get tested for HPV were included in this study. HPV screening was performed using the HPV GenoArray Test Kit and the anatomical site of sample collection is urethra. The mean age was 36.15 ± 9.08 years. Our results revealed that the prevalence of HPV infection among men was 23.8% in Yunnan Province, and there was no association between age and HPV prevalence in men. There were 72 (81.81%) individuals positive for a single HPV type and 16 (18.19%) positive for multiple types. The highest prevalent oncogenic HPV types were types 52, 51, and 16. The prevalence of HPV in men was relatively high, and the most prevalent types of HPV infection were similar between men and women from this region.Fabry disease (FD), like COVID-19, can affect multiple organs, including the lungs. Patients with FD would be expected to develop severe forms of COVID-19, not only because of lung involvement but also because of renal involvement and other comorbidities. We present two cases of patients with Fabry disease who had an infection by COVID-19. In the two cases presented, they presented mild forms of COVID-19. Although the role of the X chromosome mutation in FD on the development of severe forms of COVID-19 is unknown; in the cases presented, it is suggested that it may play a protective role in the development of COVID-19. Two cases are presented; it is suggested that FD would not be a risk factor for severe COVID-19.The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently a major concern worldwide. Guidelines have been issued regarding precautions for healthcare workers taking care of SARS-CoV-2 infected patients. In spite of accurate observance of infection control measures, including contact precautions, we report an OXA-23-producing Acinetobacter baumannii outbreak in the 5 Intensive Care Units of 10 beds each in our tertiary care teaching hospital.Continuous high-risk (HR) human papillomavirus (HPV) infection is an essential factor for cervical carcinoma and its precancerous lesions. There are differences in HPV distribution among different countries, regions and ethnic groups. ENOblock solubility dmso The aim of this research was to reveal the epidemiological characteristics of HPV in Chongqing, China. Twenty-three HPV genotypes of 13788 women aged 18 to 78 were screened by PCR-reverse dot blot hybridization. The total HPV-positive rate was 19.9% (2745/13788), while the positive rates of HR, and low-risk (LR) HPV were 17.3% (2379/13788), and 4.6% (638/13788), respectively. In addition to cervical cancer (CC) and cervical intraepithelial neoplasia (CIN) patients, the HPV infection rates of infertile women and women with gynecological diseases were markedly higher than those of healthy people. The HPV and HR-HPV infection rates in the different age groups showed statistically significant differences, and the prevalence peaks appeared in women under 20 years and over 50 years of age. HPV-52, HPV-16 and HPV-58 ranked as the top 3 most common subtypes among women in Chongqing. The results of this research provide epidemiological information on HPV infection in Chongqing. These data constitute valuable evidence for the prevention and management of cervical carcinoma and the development of HPV vaccines.Food animal production is important for every country. Several antibiotic agents are used in poultry farming to reduce the economic losses arising from mostly untested, infectious diseases. This continued study was performed to determine the prevalence of antibiotic resistant Salmonella in broiler chickens, poultry farmers, and Salmonella-bacteremia patients. A total of 121 Salmonella isolates were collected from the Thai provinces of Khon Kaen (65 isolates), Ratchaburi (43 isolates), and Phayao (13 isolates). link2 Salmonella from chicken showed a high rate of resistance to nalidixic acid and tetracycline. Sixty-four percent of Salmonella carried class 1 integron (intI1 gene positive). Among 121 Salmonella isolates, there were 15 serotypes, with Enteritidis the most common. A clonal relationship between the chicken and human isolates was demonstrated by three molecular typing methods Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction; Pulsed-Field Gel Electrophoresis; and High-Throughput, Multilocus Sequence Typing. A spread of the sequence type 11 (ST11) clone was found between chickens and humans. This study revealed a large-scale Salmonella outbreak in Thailand, a link between resistant bacteria from poultry farms and vertical transmission through the food chain, and a horizontal transmission of resistance genes. These results can be used for future surveillance and monitoring.We aimed to investigate the clonal relationships, common sequence types, and carbapenemase genes in 177 non-repetitive blood culture isolates of Acinetobacter baumannii collected from patients in three university hospitals in 2016. The molecular epidemiological characteristics of the isolates were examined with pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) (Pasteur scheme-cpn60, fusA, gltA, pyrG, recA, rplB, rpoB). Multiplex PCR was used for investigating the carbapenemase genes; including blaOXA-23-like, blaOXA-24-like, blaOXA-48-like, blaOXA-58-like, blaIMP, blaVIM, and blaNDM. PFGE genotyping yielded 92 pulsotypes with a clustering ratio of 69.7%. According to a ≥85% similarity coefficient, 159 (90.9%) isolates were found to be clonally related. The blaOXA-23-like and blaOXA-58-like genes were identified in 100% and 28.2% of the isolates, respectively. The blaNDM gene was identified in two isolates. The MLST analysis included 54 isolates with different pulsotypes and identified 29 sequence types (STs). Most of the isolates (n=36) belonged to the international clone II (CC2); one isolate belonged to CC1, and one isolate belonged to CC164. Sixteen new STs (ST1235-ST1250) were found. Identifying both global ST2 and also a high number of new STs revealed high-level genetic diversity in A. baumannii isolates in the study population.The prognostic value of interleukin-6 in COVID-19 needs to be clarified. In this retrospective study, COVID-19 patients treated in Renmin Hospital of Wuhan University from Jan 7 to Feb 8, 2020 with measurements of serum IL-6 levels within 1 week after admission were included. Data regarding demographics, clinical characteristics, laboratory tests, complications and outcomes were collected and analyzed. Sixty-six patients included (31 females) were divided into normal group (serum IL-6 less then 10pg/mL, n=35) and abnormal group (serum IL-6 ≥ 10pg/mL, n=31). Compared with normal group, the incidences of critical cases (P less then 0.001), ARDS (P=0.001), acute cardiac injury (P=0.002), cardiac insufficiency (P=0.039), mechanical ventilation rate (P=0.002) and the mortality (P=0.021) were significantly increased in abnormal group. Serum IL-6 concentration was an independent predictor for a fatal outcome (P=0.04). The optimal cutoff value of serum IL-6 concentration for predicting fatal outcomes was 26.09 pg/mL (P less then 0.001). In COVID-19, elevated serum IL-6 levels were associated with critical illness, usage of mechanical ventilation and complications including heart injury and ARDS, and could predict a fatal outcome. Early detection of serum IL-6 levels after admission would be necessary in COVID-19 patients.FIRST PLACE Activation of DNA Damage Response and Cellular Senescence in Cardiac Fibroblasts Limit Cardiac Fibrosis After Myocardial InfarctionMasato. Shibamoto, Tomoaki Higo, Atsuhiko T. Naito, Akito Nakagawa, Tomokazu Sumida, Katsuki Okada, Taku Sakai, Yuki Kuramoto, Toshihiro Yamaguchi, Masamichi Ito, Yuki Masumura, Shuichirou Higo, Jong-Kook Lee, Shungo Hikoso, Issei Komuro, Yasushi Sakata. link3 Int Heart J 2019; 60 (4) 944-957. SECOND PLACE Pressure Overload Impairs Cardiac Function in Long-chain Fatty Acid Transporter CD36-Knockout Mice. Kazuhiro Nakatani, Daisaku Masuda, Takuya Kobayashi, Masami Sairyo, Yinghong Zhu, Takeshi Okada, Atsuhiko T. Naito, Tohru Ohama, Masahiro Koseki, Toru Oka, Hiroshi Akazawa, Makoto Nishida, Issei Komuro, Yasushi Sakata, Shizuya Yamashita. Int Heart J 2019; 60 (1) 159-167. THIRD PLACE Comparison of Clinical Outcomes between the Ostial Versus Non-Ostial Culprit in Proximal Left Anterior Descending Artery Acute Myocardial Infarction. Kei Yamamoto, Kenichi Sakakura, Naoyuki Akashi, Yusuke Watanabe, Masamitsu Noguchi, Yousuke Taniguchi, Hiroshi Wada, Shin-ichi Momomura, Hideo Fujita.