Duusmunoz1779

is a parasite from the Acanthocephala phylum with worldwide distribution.

parasites are found in pinnipeds as their definitive host.

This study aimed to investigate the morphological and molecular characteristics of

, and its histopathological effect on the intestinal tissue of

.

A severe

infection was observed in the small intestine of a juvenile male Caspian seal (

). The morphological descriptions were done using light microscopy, and scanning electron microscopy (SEM). The molecular diagnosis was performed using partial sequences of internal transcribed spacer 1 (ITS1) and 5.8S ribosomal RNA (

) gene.

According to the results, the

specimens were identified as

The histopathological inspection of intestinal tissue revealed lesions in epithelial cells, mucosa, submucosa and muscle layers, destruction of intestinal glands, and infiltration of inflammatory cells.

Presence of such a severe infection in one of the individual Caspian seals can suggest the possibility of morbidity among other seals in the landlocked Caspian Sea. Thus, further research on their parasite infections is required for understating the status of the Caspian seal population and conserving this endangered species.

Presence of such a severe infection in one of the individual Caspian seals can suggest the possibility of morbidity among other seals in the landlocked Caspian Sea. Thus, further research on their parasite infections is required for understating the status of the Caspian seal population and conserving this endangered species.

Cutaneous pythiosis in horses is a chronic ulcerative granulomatous disease caused by the oomycete

.

The objective of the present study was to evaluate the response of cutaneous pythiosis in horses to surgical excision and topical dimethyl sulphoxide (DMSO).

Thirty horses were presented clinically with pruritus, fistulae discharging serosanguineous fluid, and output kunkers in different body areas (limb, abdomen, neck, and face). The clinical diagnosis was confirmed by isolation of the causative agent and histopathology. All animals were treated by surgical excision alone, or surgical excision followed by topical DMSO. The healing process was monitored every week macroscopically to evaluate the response to treatment until complete recovery.

The existence of

was confirmed in all cases. Histologically, affected horses were characterized by granulation tissue with abundant eosinophils. The size of wounds and the clinical features of pythiosis lesions decreased more after surgical debridement with DMSO application than surgical excision alone. The cutaneous pythiosis lesions were completely recovered at 35 ± 7 and 60 ± 5 days after the surgical excision with topical DMSO and surgical excision alone, respectively.

The combination of surgical excision and topical DMSO is found an effective treatment for cutaneous pythiosis in horses.

The combination of surgical excision and topical DMSO is found an effective treatment for cutaneous pythiosis in horses.

Brucellosis is a zoonosis, caused by

spp. which are small aerobic intracellular coccobacilli, localized in the reproductive organs of host animals, causing abortion and sterility. selleck inhibitor The diagnosis of this zoonosis is based on microbiological, serological or real time-polymerase chain reaction (RT-PCR) laboratory tests. Although the common microbiological and serological based assays have advantages, they are not able to solve the diagnosis problems.

To overcome some of the limitations of present techniques, in this study, we developed an aptamer through whole-cell systematic evolution of Ligands by EXponential enrichment (SELEX) procedures to detect

.

We used mixture of

and

as the target. In order to prepare the single-stranded DNA (ssDNA) aptamer, the DNA library was amplified with 5´-phosphorylated reverse primer and treated with lambda exonuclease. The SELEX procedure was performed by incubating the ssDNA pool with a bacterial suspension in a binding buffer. The selected procedures were monitored by flow cytometry using FITC-labelled forward primer. Aptamers with the highest binding affinity towards the target and the lowest to other strains were selected.

Two aptamers namely B20 and B21 showed significant binding affinity toward

and

. The dissociation constant (K

) for aptamers B20 and B21 was 40.179 ± 3.06 pM and 184.396 ± 465 pM, respectively.

The isolated aptamers were able to identify

and

with a remarkable binding efficiency and appropriated K

in a picoMolar range and therefore can be good candidates in the development of any rapid assay test implanted on routine brucellosis diagnoses.

The isolated aptamers were able to identify B. melitensis and B. abortus with a remarkable binding efficiency and appropriated Kd in a picoMolar range and therefore can be good candidates in the development of any rapid assay test implanted on routine brucellosis diagnoses.

is one of the most important enteric pathogenic bacteria that threatened poultry health.

This study aimed to investigate the prevalence and antimicrobial resistance of

isolates in goose farms.

A total of 244 cloacal swabs were collected from goose farms to detect

in Northeast China. Antimicrobial susceptibility, and resistance gene distribution of

isolates were investigated.

Twenty-one

isolates were identified. Overall prevalence of

in the present study was 8.6%. Among the

isolates, the highest resistance frequencies belonged to amoxicillin (AMX) (85.7%), tetracycline (TET) and trimethoprim/sulfamethoxazole (SXT) (81%), followed by chloramphenicol (CHL) (76.2%), florfenicol (FLO) (71.4%), kanamycin (KAN) (47.6%), and gentamycin (GEN) (38.1%). Meanwhile, only 4.8% of the isolates were resistant to ciprofloxacin (CIP) and cefotaxime (CTX). None of the isolates was resistant to cefoperazone (CFP) and colistin B (CLB). Twenty isolates (95%) were simultaneously resistant to at least two antimicrobials. Ten resistance genes were detected among which the



,

,

,

,

,

, and

were the most prevalent, and presented in all 21 isolates followed by

(20/21),

(19/21), and

(15/21).

Results indicated that

isolates from goose farms in Northeast China exhibited multi-drug resistance (MDR), harboring multiple antimicrobial resistance genes. Our results will be useful to design prevention and therapeutic strategies against

infection in goose farms.

Results indicated that Salmonella isolates from goose farms in Northeast China exhibited multi-drug resistance (MDR), harboring multiple antimicrobial resistance genes. Our results will be useful to design prevention and therapeutic strategies against Salmonella infection in goose farms.