Duncanwong0362

Our prototype platform consists of a microfluidic circuit whose hydrodynamic traps immobilize the live cuboids in arrays at the bottom of a multi-well plate. Fluid dynamics simulations enabled the rapid evaluation of design alternatives and operational parameters. We demonstrate the proof-of-concept application of model soluble compounds such as dyes (CellTracker, Hoechst) and the cancer drug cisplatin. Upscaling of the microfluidic platform and microdissection method to larger arrays and numbers of cuboids could lead to direct testing of human tissues at high throughput, and thus could have a significant impact on drug discovery and personalized medicine.Olefin 1,2-difunctionalization has emerged as a popular strategy within modern synthetic chemistry for the synthesis of vicinal amino alcohols and derivatives. The advantage of this approach is the single-step simplicity for rapid diversification, feedstock nature of the olefin starting materials, and the possible modularity of the components. Although there is a vast number of possible iterations of 1,2-olefin difunctionalization, 1,2-amino oxygenation is of particular interest due to the prevalence of both oxygen and nitrogen within pharmaceuticals, natural products, agrochemicals, and synthetic ligands. The Sharpless amino hydroxylation provided seminal results in this field and displayed the value in achieving methods of this nature. However, a vast number of new and novel methods have emerged in recent decades. This review provides a comprehensive review of modern advances in accomplishing 1,2-amino oxygenation of alkenes, 1,3-dienes, alkynes, and allenes that move beyond osmium to a range of other transition metals and more modern strategies such as electrochemical, photochemical, and biochemical reactivity.Owing to its rapid response and broad detection range, a phenylboronic acid (PBA)-functionalized hydrogel film-coated quartz crystal microbalance (QCM) sensor is used to non-invasively monitor salivary glucose in diabetic patients. However, nonspecific protein adsorption on the PBA-functionalized hydrogel film can cause dramatic loss of sensitivity and accuracy of the sensor. A traditional zwitterionic polymer surface with ultra-low protein fouling can hinder the interaction of PBA in the hydrogel matrix with glucose molecules owing to its steric hindrance, resulting in poor glucose sensitivity of the sensor. Herein, we developed a novel hydrogel film that enhanced the antifouling properties and sensitivity of the QCM sensor by infiltrating a glucose-sensitive monomer (i.e., PBA) into a zwitterionic polymer brush matrix to form an interpenetrating polymer network (IPN). The IPN hydrogel film could minimize the glucose sensitivity loss since the antifouling polymer distributed in its matrix. Moreover, a stable hydration layer was formed in this film that could prevent water from transporting out of the matrix, thus further improving its antifouling properties and glucose sensitivity. The experimental results confirmed that the IPN hydrogel film possessed excellent resistance to protein fouling by mucin from whole saliva with reductions in adsorption of nearly 88% and could also enhance the glucose sensitivity by nearly 2 fold, compared to the PBA-functionalized hydrogel film. Therefore, the IPN hydrogel film provides improved antifouling properties and sensitivity of the QCM sensor, which paves the way for non-invasive monitoring of low concentrations of glucose in saliva.Slippery liquid-infused porous surfaces inspired by the Nepenthes pitcher plant exhibit excellent performances and are known for their extremely low contact angle hysteresis ( less then 5°) and smooth surface. In contrast, superhydrophobic surfaces (SHS) exhibit poor pressure stability, difficulty in self-healing, and difficulty in removing low surface tension liquids or organic solvents, which can affect the stable air layer. Thus, these issues can be avoided through the replacement of SHS with slippery liquid infused porous surfaces (SLIPS). In this review, the theoretical models of SHS and SLIPS are classified initially, and several design standards for the preparation of SLIPS are briefly described. Then, we focus on comparing the differences in the application of SHS and SLIPS, such as pressure stability, transparency, and droplet manipulation. However, there are still some problems that need to be improved during the preparation of SLIPS, such as the evaporation of the lubricant layer, the use of a lubricant layer of toxic perfluoropolyether and other substances, and easily lost nanostructured lubricant layer. Accordingly, several new improved methods are proposed in this review, and finally, the potential applications and development prospects of SLIPS are presented.Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an etiological agent of the current rapidly growing outbreak of coronavirus disease (COVID-19), which is straining health systems around the world. Disrupting the intermolecular association of SARS-CoV-2 spike glycoprotein (S protein) with its cell surface receptor human angiotensin-converting enzyme 2 (hACE2) has been recognized as a promising therapeutic strategy against COVID-19. The association is a typical peptide-mediated interaction, where the hACE adopts an α1-helix, which can form a two-helix bundle with the α2-helix, to pack against a flat pocket on the S protein surface. Here, we demonstrate that the protein context of full-length hACE plays an essential role in supporting the hACE2 α1-helix recognition by viral S protein. Energetic analysis reveals that the α1-helical peptide (αHP) and also the two-helix bundle peptide (tBP) cannot bind effectively to S protein when they are split from the hACE protein. The context contributes moderattate to facilitate the conformational selection by S protein.Functionalized carbon nanotubes are interesting, promising and unique delivery systems for anticancer drugs, which are now in the spotlight of nanomedicine. Connecting nanotubes with anticancer drugs or new compounds with anticancer properties aims at improving their stability, efficiency and reduces the toxic side effects of cancer treatment. In our research, we are interested in connecting functionalized MWCNTs-NH2 with [InH][trans-RuCl4(In)2], (KP1019) which is one of the most promising anticancer ruthenium(iii) drug candidates, known mainly as a cytotoxic agent for the treatment of platinum-resistant colorectal cancers. As a result of the amidation of MWCNTs (1), MWCNTs-NH2 (2) were obtained. Then, they were modified with [InH][RuCl4(In)2] (4) and the nanosystem [MWCNT-NH3+][RuCl4(In)2-] (3) was obtained. The characterization of the resulting products was performed using IR, Raman spectroscopy, thermal gravimetric, XRD, STEM-EDX, ESI-MS, ICP-MS, and XPS analyses. The cytotoxic activity has been tested on human lung carcinoma (A549), chronic myelogenous leukemia (K562) and human cervix carcinoma (HeLa) cells which showed the higher toxicity of the nanosystem than the ruthenium complex.We report herein experimental observations of the reciprocating motion of a self-propelled droplet floating on the surface of an aqueous surfactant solution and a simple reaction model capable of reproducing the observed behavior of the droplet. The reciprocating motion was observed in a quasi-one-dimensional annular channel, so the reciprocation was not caused by reflections at boundaries. To understand the reciprocation, our model assumes a reaction between the surface active substance emitted from the droplet and surfactants dissolved in the aqueous solution. IPA3 This reaction invokes an inversion of the surface tension gradient and thus the droplet's reciprocation. We show that the model can reproduce experimental results semi-quantitatively using numerical simulations with realistic parameters.Products containing probiotics are targeted at healthy or at-risk individuals as a preventative measure to minimise disease risk. Most studies assessing the efficacy of probiotics in humans include a mixture of healthy and unhealthy populations, while studies that focus solely on female populations are largely limited to pregnancy or those with health conditions. Pre-conception is a significant time-point during the life-course, and improving female health status during this period may positively influence future offspring. The objective of this review is to assess the effect of probiotics administered in oral capsule formulation, on metabolic and immune markers in healthy, non-pregnant women of reproductive age. This review followed the PRISMA guidelines. Pubmed, EMBASE, CINAHL, and Web of Science were searched for relevant studies. English language articles relating to randomised-controlled trials were included. The search returned 3250 publications after duplicates were removed. Title (2516), abstract (642), and full text (87) screening excluded 3993 studies from consideration. Five papers were identified with outcomes of interest, and analysis of these showed no conclusive evidence that probiotic capsule supplementation elicited positive effects in this healthy population. This study highlights the need for further research to investigate the role that probiotics play during the pre-conception period, on female metabolic and immune health.For the inhibition of myostatin, which is an attractive strategy for the treatment of muscle atrophic disorders including muscular dystrophy, myostatin-binding peptides were synthesized with an on/off-switchable photooxygenation catalyst at different positions on the peptide chain. These functionalized peptides oxygenated and inactivated myostatin upon irradiation with near-infrared light. Among the peptides tested, a peptide (5) with the catalyst moiety at the 16 position induced myostatin-selective photooxygenation, and efficiently inhibited myostatin. These peptides exhibited low phototoxicity. Such functionalized peptides would provide a precedented strategy for myostatin-targeting therapy, in which myostatin is irreversibly and catalytically inactivated by photooxygenation.Here, we propose a planar hot-electron photodetector based on broadband Tamm plasmon resonance using a TiN layer, n-type doped Si layer, and seven pairs of DBRs. Simulation results show high absorption (94.2%) with a full width at half maximum of 239.3 nm, which is 2.9 times that of the Au/DBR configuration. We predict that the photoresponsivity can reach 26.1 mA W-1 at 1140 nm. Since the planar nanofilms for TP resonance are facile to fabricate, this work promotes hot-electron applications in broadband photodetection and other broadband light-harvesting applications.A large number of plants and fungi are homologs of medicine and food, and are used in the form of decoctions for complementary foods, dietary cures, and disease therapy in traditional medicine. Besides the widespread concern around the physiological and pharmacological actions of the active ingredients, the phase change in decoction and its influences on the active ingredients' absorption should not be ignored. Lots of particle aggregates are generated during the decoction of herbal medicine and then end up being taken together with the active ingredients. The question arises, "Is the absorption of active ingredients associated with the particle aggregates in decoction?" The present study takes the Coptis chinensis decoction (CCD), the particle aggregates in CCD (CCD-Ps), and the water-insoluble active ingredient of Berberine (Ber) as typical examples to investigate the effects of particle aggregates in herbal medicine decoction on the active ingredient absorption in the intestine and the underlying mechanisms.