Dudleyfagan1691

OBJECTIVE To determine if a dynamic embryo culture system affects the reproductive potential of human embryos resulting from in vitro fertilization (IVF). DESIGN Paired randomized controlled trial (RCT). SETTING IVF center. PATIENT(S) IVF patients with normal ovarian reserve eligible for two-embryo transfer. INTERVENTION IVF care was routine until fertilization was confirmed. Two-pronuclear embryos (2PNs) were then randomized One-half of each patient's 2PNs were cultured in dynamic culture and one-half in static culture. Preimplantation genetic testing for embryonic aneuploidy was used to control for aneuploidy and allow for DNA fingerprinting. KN-62 price The best euploid blastocyst from each culture system was selected and patients underwent a frozen two-embryo transfer. If a singleton gestation resulted, DNA-fingerprinting was used to determine which of the two blastocysts implanted. The dynamic platform used was the NSSB-300 (Nepagene). MAIN OUTCOME MEASURE(S) The primary outcome was the proportion of usable blastocysts obtained. The secondary outcome was sustained implantation rate (SIR). RESULT(S) One hundred participants completed oocyte retrieval and blastocyst vitrification for frozen-thawed embryo transfer; 609 dynamic 2PNs and 615 static 2PNs were followed; and 304 blastocysts developed in dynamic culture and 333 blastocysts developed in static culture. In the paired analysis, the rate of usable blastulation was similar between dynamic and static culture (58.3% vs. 57.1%). In addition, there was no difference in the rate of aneuploidy (20.0% vs. 33.3%) or SIR (67.1% vs. 63.1%) between groups. CONCLUSION(S) In this paired RCT, dynamic culture did not improve usable blastulation rate or SIR. CLINICAL TRIAL REGISTRATION NUMBER NCT02467725. A novel back recursive estimation (BRE) scheme is proposed for superconducting radio frequency (SRF) cavities. Microphonic,the main source of cavities detuning is modeled as unknown frequency sinusoidal disturbance. The disturbance property is excited by an auxiliary filter and the frequency information is estimated in observer framework. Furthermore, the sinusoidal disturbance is rearranged as a series of dynamics form using virtual disturbances. Back recursive signal is calculated according to the correlation between virtual disturbance and equivalent input disturbance. As a result, the asymptotic stability of estimation error can be obtained based on Lyapunov function, and robustness can be obtained if another external bounded disturbance exists. Simulations verify the effectiveness of the proposed method. BACKGROUND Living donation is a potential source of organs that could help to reduce the organ transplant deficit. Given that we have a worldwide aging population, it is important to assess the opinion of older people toward this type of donation. OBJECTIVES To analyze the attitude of people aged > 65 years toward living kidney donation (LKD) and living liver donation (LLD) and to investigate the variables affecting their attitudes. METHODS A multicentric study was carried out using a representative sample of people > 65 years stratified by sex and geographic location in southeastern Spain (n = 420). The measurement instrument was a validated questionnaire about LKD and LLD. Statistics were analyzed using SPSS version 21.0 (IBM Corp, Armonk, NY, United States) software. Descriptive analysis was carried out using Student t test, χ2 test, and a multivariate analysis. RESULTS The questionnaire completion rate was 84% (n = 351) with 88% (n = 310) in favor of LKD, and 89% (n = 311) in favor of LLD. Favorable attitude decreased to 3% when the donation under consideration was unrelated. Attitudes toward LKD and LLD were associated with having received information from the television (P = .016 and P = .045) and from friends (P = .017 and P = .03); accepting an autopsy after death (P = .001 and P = .002); and not being worried about scars (P = .015 and P = .044). In the multivariate analysis, the following variables continued to be significant having received information from the television (odds ratio [OR], 2) and from friends (OR, 10.3); and the acceptance of an autopsy (OR, 2). CONCLUSIONS Older people are in favor of both LKD and LLD, assuming it is a related donation. In addition, the information the elderly population receives regarding organ donation and transplantation affects their attitudes. INTRODUCTION Algeria is an important source of immigrants to Europe. A population with a low culture of donation and transplantation and little knowledge of related concepts such as brain death (BD). OBJECTIVE Analyze the knowledge and acceptance of the BD concept of the population born in Algeria and residing in Spain. MATERIAL AND METHODS Population under study. Population born in Algeria and resident in Spain. INCLUSION CRITERIA Sample of population over 15 years stratified by age and sex. INSTRUMENT Attitude validated questionnaire for organ donation for transplant PCID-DTO-Ríos. Field work. Random selection of people to be surveyed according to stratification. RESULTS A total of 441 respondents have been included in the study; of these, 27.2% know the concept of BD and accept it as a person's death. Of the rest, 18% do not know or have doubts about the concept, and 54% have a misconception or do not accept it as the death of a person. Those who know the concept of BD have a more favorable attitude toward donation (P = .001). When making the correlation to see which psychosocial factors are related to the knowledge of the concept of brain death, it is obtained that those respondents who have spoken with family about organ transplantation have a better knowledge of it (P  less then .001). CONCLUSIONS The Algerian population immigrating to Spain has little knowledge of the BD concept, which has a direct relationship to their attitude toward organ donation. The family-level approach to the donation and transplant process sensitizes and improves the knowledge of this concept. BACKGROUND Worldwide population aging has resulted in changes in the approach to the organ donation and transplantation (ODT) process, forcing us to include older people on transplant waiting lists and to assess older patients as potential donors. However, this is a sector of the population that has not been studied in great detail in terms of the information they receive about ODT. OBJECTIVES To analyze what kinds of media provide people > 65 years of age with information about ODT and which sources of information affect their attitude about this subject. METHODS A multicentric study was undertaken using a sample of people > 65 years of age stratified by sex and geographic location in southeastern Spain (n = 420). Questions about ODT and methods of receiving information were analyzed using a questionnaire. Statistical analysis was performed using SPSS version 21.0 (IBM Corp, Armonk, NY, United States). Descriptive analyses were performed with a Student t test and χ2 test. RESULTS The questionnaire completion rate was 84% (n = 351).