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Global urbanization has resulted in amplified energy and material consumption with simultaneous waste generation. Current energy demand is mostly fulfilled by finite fossil reserves, which has critical impact on the environment and thus, there is a need for carbon-neutral energy. In this view, biohydrogen (bio-H2) is considered suitable due to its potential as a green and dependable carbon-neutral energy source in the emerging 'Hydrogen Economy'. Bio-H2 production by dark fermentation of biowaste/biomass/wastewater is gaining significant attention. However, bio-H2production still holds critical challenges towards scale-up with reference to process limitations and economic viabilities. This review illustrates the status of dark-fermentation process in the context of process sustainability and achieving commercial success. The review also provides an insight on various process integrations for maximum resource recovery including closed loop biorefinery approach towards the accomplishment of carbon neutral H2 production.

The range of normal values for coagulation factors in the healthy newborn was described 30 years ago but since then the reagents, automated systems, and dosing techniques have changed considerably. For 30 years, several authors have tried to update the standards and references in children using updated reagents but the newborn and infant population in these studies has been quite small, limiting the findings. The aim of this study was to establish the normal coagulation standards in healthy newborns.

We included all consecutive healthy newborns with pyloric stenosis presenting to our reference center over a period of 5 years. We calculated the reference ranges defined as mean±2 SD. Normality of distribution was checked graphically and by using the Shapiro-Wilk test. Correlations between two continuous variables were assessed using Spearman's rank coefficient correlation. Statistical testing was done at the two-tailed α-level of 0.05. Data were analyzed using the SAS software package, release 9.4 (SAS Instablish standards for coagulation testing in this very specific population. Indeed, our study represents the largest newborn population in a recent investigation of PT and aPTT using updated reagents.Testicular torsion (TT) occurs when the testis rotates around the axis of the spermatic cord attachments and prevents blood flow to the testis, resulting in tissue ischemia. If this occurs in the first month of life it is referred to as "perinatal TT" (PTT) or "neonatal TT" (NTT). PTT has an incidence of 6.1 per 100,000 live births. Some of these cases occur prenatally. It can be missed on the initial newborn examination, as it can be asymptomatic. Hence, the true incidence is much higher since it is underdiagnosed. The types of TT include extravaginal, intravaginal, and mesorchial. Most cases of PTT are extravaginal. The diagnosis can generally be made on physical examination. Ultrasonography (US) can help exclude other rare diagnoses as long as surgical intervention is not delayed. There has been some debate regarding the timing of surgery. Although the torsed testicle may not be salvageable, the likelihood of asymptomatic bilateral TT has to be borne in mind and contralateral orchiopexy done at the time of exploration would prevent an asynchronous torsion. Pitstop 2 clinical trial Nonoperative maneuvers to detorse in PTT are not recommended. The evaluation, diagnostic approach, and management of this relatively rare condition are described.

Singing in a choir can play a significant role in a strategy for lifelong health. This study aimed to assess the nutritional status and the quality of life of healthy adults singing in a choir.

The cross-sectional study, carried out from August 2017 to June 2018, was preceded by a weekly pilot study. A total of 100 choristers (72 women, 72.0%) aged 19-70 years (mean age was 38.30 ± 15.99 years) were included in the study. The control group consisted of 100 healthy adults (68 women, 68.0%) aged 19-70 years (mean age was 38.48 ± 16.09).

Significantly lower body weight and body mass index and significantly lower results of selected body composition components (P< 0.05) were found in the study group in comparison with the control group. The choristers showed significantly lower values of basal metabolic rate (P= 0.004) and metabolic age (P< 0.001). In addition, a significantly higher quality of life was observed in the group of choristers in each of the domains tested (P < 0.05).

Singing in the choir may have effect on lifelong health, defined as better nutritional status and better perception of quality of life and health.

Singing in the choir may have effect on lifelong health, defined as better nutritional status and better perception of quality of life and health.

The voice is an important marker of the transition process to the new gender identity of the transgender person. For 20% of patients seeking voice feminization, voice therapy is not completely satisfactory, and surgery should be considered, with endoscopic glottoplasty being the current practice. While the increase in fundamental frequency (F0) after glottoplasty has been well described, no systematic review of the literature or meta-analysis regarding other acoustic parameters and quality of voice has been performed yet.

To define the effect of endoscopic glottoplasty on acoustic measures and quality of voice by assessing F0, maximum phonation time (MPT), frequency range, and grade of dysphonia.

A literature review was performed in Medline/PubMed, Cochrane, Science Direct, LILACS, and Google Scholar, following PRISMA guidelines, with no constraints on publication date. We included studies in English, Portuguese or Spanish that assessed transgender women who underwent endoscopic glottoplasty. All the included articles were measured in terms of their methodological quality.

The PRISMA approach yielded 14 studies, totaling 566 patients. There was significant heterogeneity between studies regarding follow-up time, surgical technique and perioperative care. Thirteen studies were submitted to meta-analysis. The results showed significant changes in pre- to postglottoplasty mean differences of F0 = 78.49 Hz (95%CI 75.69-81.30), MPT = -1.11 seconds (95%CI -1.67 to -0.54), frequency range = -3.55 semitones (95%CI -5.74 to -1.36) and grade of dysphonia on the GRBAS scale = 0.44 (95%CI 0.27-0.61).

Glottoplasty is effective in significantly increasing fundamental frequency, but slightly decreases MPT, frequency range and vocal quality measured by the grade of dysphonia on the GRBAS scale.

Glottoplasty is effective in significantly increasing fundamental frequency, but slightly decreases MPT, frequency range and vocal quality measured by the grade of dysphonia on the GRBAS scale.A novel innovative viscoelastic gelling agent (novel gel, NG) has been developed by combining citric acid (CA) and disodium 5-guanylate (DG). NG has the potential to replace other gelling agents such as gelatine, which has been commonly used in foods, dietary supplements, pharmaceutical and cosmetic products including ointments and sprays. NG has unique physico-chemical properties, including a wide range of concentration-dependent, temperature-sensitive gel strengths. Based on the rheological measurement results, NG depicted similar shear thinning behaviour to gelatine, within shear rates ranging from 25.8 to 129 (s-1). NG also significantly increased the shelf-life (by 21 days) of minced beef, as well as inhibited the growth of major spoilage pathogens, such as E. coli, S. aureus, Salmonella sp., Listeria sp., yeast and moulds, making it an ideal candidate for gelatine replacement.Vitamin D (VD) is a fat-soluble vitamin with high deficiency levels evident globally. Bioaccessibility of VD is influenced by formation of mixed micelles (MM) during digestion. This study assessed the impact of fatty acid (FA) type, phospholipid concentration on MM formation and stability of MM to salts. MM formation occurred at NaCl and KCl concentrations ranging from 20 to 100 mM, when octanoic acid (C8) or stearic acid (C18) were used. MM hydrodynamic size increased with increasing l-α-phosphatidylcholine concentration (1.5-7.5 mM) for both C8 and C18, above which concentration MM did not form. FA chain length impacted MM with hydrodynamic size increasing from 3.8 nm for decanoic acid (C10) to 4.4 nm for C18. VD3 incorporation in MM was not influenced by the FA used (C10 or C18). Understanding stability and formation of MM and VD3 loading is an essential first step towards manipulating food structures for improving delivery of VD.In this study, the effect of two drying methods (conductive hydro-drying - CHD and freeze-drying - FD) on the physical and functional properties of green gram (GG) and black gram (BG) protein powders was investigated. CHD dried protein powder showed excellent powder characteristics with moisture contents ranging from 3 to 6%, water activity of ~0.4 and Carr index ≤10. The CHD samples were dried in 210 min; with higher drying rates, CHD samples showed no significant changes in powder characteristics, color value, and water and oil absorption indices. The solubility of both proteins were found to be lower at pH 4 to 7 and higher at pH 1, 2, 8, 9 and 10; at certain pH, the solubility of CHD protein was higher than that of the FD counterparts. No significant differences were observed in the oil absorption capacity, surface hydrophobicity, protein gel formation, pasting and thermal properties. XRD and FTIR analyses were used to explain changes in protein structure and the presence of both α-helix and β-sheet was observed, with higher β-sheet levels in both pulses dried using CHD. Results confirmed that CHD, a variant of the refractance window drying (RWD) offered protein quality in par with FD.This study was conducted to evaluate the inactivation of Bacillus cereus spore in mesquite flour with intense pulsed light (IPL) and gamma radiation. The physical, chemical, and toxicity of treated mesquite flour were also investigated. The results showed that up to 3.51 log10CFU/g B. cereus spore inactivation was achieved with 8 kGy of gamma radiation, and up to 1.69 log10CFU/g reductions could be achieved after 28s of catalytic IPL exposure. Although chemometric analysis showed 9-hydroxy-10,12-octadecadienoic acid was slightly increased after a 28s-catalytic IPL treatment, the concentration is within the acceptable range. No significant increase in acetic or propionic acids (typical off-flavor volatile compounds) was observed after either treatment. For cytotoxicity, the Caco-2 cell viability analysis revealed that these two technologies did not induce significant cytotoxicity to the treated mesquite flour. Overall, these two technologies exhibit strong potential for the decontamination of B. cereus in mesquite flour.In this work, capsaicin (CAP) was loaded into mesoporous silica nanoparticles (MSNs) with grain diameters of 50 nm, 100 nm and 400 nm by free diffusion to obtain the support materials Cap@MSN-50, Cap@MSN-100 and Cap@MSN-400, respectively. The loading capacity was separately calculated as 854.77, 713.86 and 649.09 (mg/g Cap/MSN) by ultraviolet spectrophotometry. An IFFM-E flow injection chemiluminescent analyzer was used for the slow-release experiment, which demonstrated that the MSN release of CAP proceeded with a slow-release effect and MSNs with a smaller grain diameter had a higher rate of slow release and stronger oxidation resistance. The prepared materials were used to maintain the freshness of ground beef by modified atmosphere packaging. After more than 5 days of storage, Cap@MSN-50 retained better oxidation resistance than CAP. The results show that loading CAP into mesoporous nanomaterials has the potential application value for long-term preservation and oxidation prevention of some foods.

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