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CONCLUSIONS A drastic difference in the number of survivors and cellular ultrastructure was observed between vegetative and air or food-dried S. Enteritidis cells after subjecting to heat treatment at 80°C. No significant ultrastructure changes were observed in desiccated cells after heat treatment except for roughening and corrugating surfaces. SIGNIFICANCE AND IMPACT OF THE STUDY This study provides a direct comparison to illustrate how desiccation influences the cell ultrastructure before/after heat treatment, which will aid in better understanding of the fundamental mechanism underlying the increased thermal resistance of Salmonella cells in low-aw environment. © 2020 The Society for Applied Microbiology.Satellite data indicate significant advancement in alpine spring phenology over decades of climate warming, but corresponding field evidence is scarce. It is also unknown whether this advancement results from an earlier shift of phenological events, or enhancement of plant growth under unchanged phenological pattern. By analyzing a 35-year dataset of seasonal biomass dynamics of a Tibetan alpine grassland, we show that climate change promoted both earlier phenology and faster growth, without changing annual biomass production. Biomass production increased in spring due to a warming-induced earlier onset of plant growth, but decreased in autumn due mainly to increased water stress. Plants grew faster but the fast-growing period shortened during the mid-growing season. These findings provide the first in situ evidence of long-term changes in growth patterns in alpine grassland plant communities, and suggest that earlier phenology and faster growth will jointly contribute to plant growth in a warming climate. © 2020 The Authors. Ecology Letters published by CNRS and John Wiley & Sons Ltd.BACKGROUND This study assessed predictors of pulmonary thromboembolism (PE) resolution and their implications for clinical outcome. METHOD A total of 150 patients with acute PE diagnosed by computed tomography pulmonary angiography (CTPA) were included. All patients received anticoagulant therapy for 3-6 months and were followed-up for at least 2 years. d-dimer levels in plasma were assayed at the first admission and during follow-up. RESULTS The rate of CTPA-confirmed PE resolution was 48.67% at 6 months, 68% at 12 months, and 78.67% at 24 months. Thirty-nine patients had recurrent thrombosis after anticoagulation therapy was stopped, whereas 93 patients had complete resolution. The initial d-dimer level positively correlated with the pulmonary artery obstruction index (PAOI) (r = 0.21; P = 0.015), but did not significantly differ between patients experiencing resolution or recurrence. In contrast, the follow-up mean d-dimer level was significantly higher in the recurrent group (P  less then  0.001), and this level was an independent risk factor for recurrent PE after the termination of anticoagulation treatment (OR 1.003, 95%CI 1.002 to 1.004; P  less then  0.001). Higher initial thromboembolic burden measured by PAOI was associated with residual thromboemboli (P = 0.004) and recurrence (P = 0.03), but was not an independent risk factor for either. CONCLUSIONS Elevated d-dimer is an independent risk factor for PE recurrence. A higher initial thromboembolic burden may be associated with unresolved thromboemboli or recurrence. © 2020 John Wiley & Sons Ltd.BACKGROUND Proximal junctional kyphosis (PJK) can cause significant functional impairment and neural compression. Varying rates of PJK and pseudoarthrosis following posterior instrumentation and fusion for adolescent idiopathic scoliosis (AIS) are described with multiple biologic and biomechanical correlations attributed. ARRY-382 supplier This retrospective study aims to determine our rate of pseudoarthrosis and PJK in posterior spinal fusion for AIS, along with analysing the influence of autograft and allograft bone volume. METHODS Immediate and 12-month post-operative radiographs of 78 patients treated for AIS were analysed along with late complications to a minimum of 2 years. Proximal kyphosis was determined by measuring and comparing the angle between the upper instrumented vertebra and upper instrumented vertebra + 2 for both immediate and 12-month post-operative radiographs. Spinal fusion was determined using an accepted grading scale on the 12-month radiograph. These findings were correlated with known surgical variables in bone grafting technique. RESULTS There was one case of PJK and no cases of pseudoarthrosis. Three patients showed a defect in the fusion mass but were still suggestive of fusion. The rates of fusion and PJK were not significantly different when using different allograft volumes or incorporating autograft. CONCLUSION Relatively low rates of PJK following AIS correction were observed compared to the literature. Rates were not increased with the use of proximal autograft. The amount of allograft used did not affect fusion rates either. © 2020 Royal Australasian College of Surgeons.BACKGROUND AND AIMS Non-O blood type (BT) is a risk factor for thromboses, which has been attributed to its effects on von Willebrand factor (VWF)/factor VIII (FVIII) levels. Although high VWF/FVIII may be risk factors for portal vein thrombosis (PVT) in patients with advanced chronic liver disease (ACLD), the impact of BT on PVT is unknown. We aimed to assess (I) whether non-O-BT is a risk factor for PVT and (II) whether non-O-BT impacts VWF/factor VIII in patients with ACLD. METHODS Retrospective analysis comprising two cohorts (I) "US" including all adult liver transplantations in the US in the MELD era and (II) "Vienna" comprising patients with a hepatic venous pressure gradient (HVPG) ≥6 mmHg. RESULTS (I) The "US cohort" included 84 947 patients (non-O 55.43%). The prevalence of PVT at the time of listing (4.37% vs 4.56%; P = .1762) and at liver transplantation (9.56% vs 9.33%; P = .2546) was similar in patients with O- and non-O-BT. (II) 411 patients were included in the "Vienna cohort" (non-O 64%). Mean HVPG was 18(9) mmHg and 90% had an HVPG ≥10 mmHg. Patients with non-O-BT had slightly increased VWF levels (318(164)% vs 309(176)%; P = .048; increase of 23.8%-23.9% in adjusted analyses), but this difference was driven by patients with less advanced disease. However, non-O-BT explained only 1% of the variation in VWF and had no effect on FVIII. CONCLUSIONS Although non-O-BT impacts VWF in patients with early stage ACLD, its contribution to VWF variation is considerably smaller than in the general population. Moreover, non-O-BT had no impact on FVIII. These findings may explain the absence of an association between non-O-BT and PVT in patients with advanced cirrhosis. ©2020 The Authors. Liver International published by John Wiley & Sons Ltd.