Dealshaffer3563

The commitment of the teams, tutoring are success factors.

Primary intracranial pure endodermal sinus tumor (EST) is a rare malignant nongerminomatous germ cell tumor. There is still lack of knowledge about the treatment and prognosis.

We studied 6 cases of primary intracranial pure EST patients in Beijing Tiantan Hospital from June 2009 to June 2015. A comprehensive literature search was performed on Pubmed's electronic database using the search terms "primary intracranial endodermal sinus tumor", "primary intracranial yolk sac tumor" and "malignant germ cell tumor". Survival rates based on age, gender, tumor location, treatment, resection degree and metastatis were collected and analyzed.

Total resection was achieved in three patients and subtotal resection was achieved in three patients. Blood AFP level continuously decreased after tumor removal. In our case series, the longest survival time was 99 months; this patient received surgery+adjuvant therapy. Fifty-two cases were eligible and met the inclusion criteria for the review analysis. The majority of the population were in the young child to adolescent age and there is a male predominance in this study. In the Kaplan-Meier survival analysis, age, gender, tumor location and metastasis did not affect patients' OS. Better OS was found in patients who had subtotal and gross total resection of the tumor. Patients who had surgery combined with adjuvant therapy showed better OS compared to those who just had surgery.

Total resection of the tumor is necessary and EST is sensitive to adjuvant therapy.

Total resection of the tumor is necessary and EST is sensitive to adjuvant therapy.

Several scientific papers report clinical symptoms, indications, complications and outcomes of brainstem cavernous malformation (BSCM) surgery without reporting on the occurrence of postoperative Holmes tremor (HT). Our purpose is to report our experience with HT in a monocentric series of resected brainstem cavernomas.

We reviewed all the BSCM surgical records between 2002 and 2018 at Saint-Luc University Hospital's Department of Neurosurgery, Brussels and selected patients developing HT postoperatively. Patients' demographics, symptoms, pre- and postoperative imaging, recurrence and complications were analysed. A PubMed literature review was performed to compare our results with those in the existing literature.

In a total series of 18 resected BSCM, 5 patients 1 male and 4 females, with a median age of 51 years (range 29-59 years), developed HT. The median preoperative mRS score was 2 (range 1-4). GTR was achieved in all patients without surgery-related death. BSCM were located in the mesencephalon in 4 patients (80%) who developed HT. Tremor was noticed between ten days and one year after surgery. One patient saw significant improvements to the point of stopping treatment. The median follow-up period was 2 years (range 1-14 years). At the last follow-up, 40% of our patients showed a worse mRS score, 40% stayed unchanged, and 20% improved.

We are reporting an original single-center series of patients suffering from HT after BSCM surgery. The risk for HT after surgery is significant for midbrain BSCM. A spontaneous favorable evolution is possible.

We are reporting an original single-center series of patients suffering from HT after BSCM surgery. The risk for HT after surgery is significant for midbrain BSCM. A spontaneous favorable evolution is possible.Neural circuits are composed of multitudes of elaborately interconnected cell types. Understanding neural circuit function requires not only cell-specific knowledge of connectivity, but the ability to record and manipulate distinct cell types independently. Recent advances in viral vectors promise the requisite specificity to perform true "circuit-breaking" experiments. However, such new avenues of multiplexed, cell-specific investigation raise new technical issues one must ensure that both the viral vectors and their transgene payloads do not overlap with each other in both an anatomical and a functional sense. This review describes benefits and issues regarding the use of viral vectors to analyse the function of neural circuits and provides a resource for the design and implementation of such multiplexing experiments.

Zebrafish (Danio rerio) are increasingly being used to model anxiety. A common behavioral assay employed for assessing anxiety-like behaviors in zebrafish is the "novel tank test". Epacadostat We hypothesized that using deeper tanks in this test would result in greater between-individual variation in behavioral responses and a more 'repeatable' assay.

After mapping the literature and identifying common behavioral parameters used in analysis, we performed novel tank anxiety tests in both custom-designed 'tall' tanks with increased depth and 'short' trapezoidal tanks. We compared the repeatability of the behavioral parameters between tall and short tanks and also investigated sex differences.

Overall, regardless of tank depth, almost all behavioral parameters associated with anxiety in zebrafish were significantly repeatable (R = 0.24 to 0.60). Importantly, our tall tanks better captured between-individual differences, resulting in higher repeatability estimates (average repeatability tall tanks R = 0.46; average repack the sensitivity to detect subtle, yet important, information, such as between-individual variation, an important component in assessing the reliability of behavioral data.

Alterations of the neurofibromatosis type 2 gene (NF2) occur in more than fifty percent of sporadic meningiomas. Meningiomas develop frequently in the setting of the hereditary tumor syndrome NF2. Investigation of potential drug-based treatment options has been limited by the lack of appropriate in vitro and in vivo models.

Using Crispr/Cas gene editing, of the malignant meningioma cell line IOMM-Lee, we generated a pair of cell clones characterized by either stable knockout of NF2 and loss of the protein product merlin or retained merlin protein (transfected control without gRNA).

IOMM-Lee cells lacking NF2 showed reduced apoptosis and formed bigger colonies compared to control IOMM-Lee cells. Treatment of non-transfected IOMM-Lee cells with the focal adhesion kinase (FAK) inhibitor GSK2256098 resulted in reduced colony sizes. Orthotopic mouse xenografts showed the formation of convexity tumors typical for meningiomas with NF2-depleted and control cells.

No orthotopic meningioma models with genetically-engineered cell pairs are available so far.

Our model based on Crispr/Cas-based gene editing provides paired meningioma cells suitable to study functional consequences and therapeutic accessibility of NF2/merlin loss.

Our model based on Crispr/Cas-based gene editing provides paired meningioma cells suitable to study functional consequences and therapeutic accessibility of NF2/merlin loss.The evolution of cooperation has been one of the main topics in evolutionary biology. If cooperators maintain interaction with cooperators and halt interaction with defectors, then cooperation can pay and can be favored by natural selection. This is called an exit option. Here, not only cooperation in dyadic interactions but also cooperation in sizable groups can be observed. Rivalry is about whether usage of the benefit by one individual reduces its availability to others or not. A common good is a rivalrous good, whereas a public good is a non-rivalrous good. In this paper, by analyzing n-player prisoner's dilemma games, we examine whether the effect of the group size on cooperation is positive or negative in the context of exit option. When goods are common goods, defectors always dominate cooperators when the group size is infinitely large. Thus, the group size has only negative effects on the evolution of cooperation when goods are common goods. In contrast, when goods are public goods, an increase in group size has positive effects as well as negative effects on the evolution of cooperation. In addition, we reveal that it has both positive and negative effects on the evolution of cooperation for cooperators to tolerate some defection and hope to keep the interaction.Spatial synchrony of population fluctuations is an important tool for predicting regional stability. Its application to natural systems is still limited by the complexity of ecological time series displaying great variation in the frequency and amplitude of their fluctuations, which are not fully resolved by current ecological theories of spatial synchrony. In particular, while environmental fluctuations and limited dispersal can each control the dynamics of frequency and amplitude of population fluctuations, ecological theories of spatial synchrony still need to resolve their role on synchrony and stability in heterogeneous metacommunities. Here, we adopt a heterogeneous predator-prey metacommunity model and study the response of dispersal-driven phase locking and frequency modulation to among-patch heterogeneity in carrying capacity. We find that frequency modulation occurs at intermediate values of dispersal and habitat heterogeneity. We also show how frequency modulation can emerge in metacommunities of autonomously oscillating populations as well as through the forcing of local communities at equilibrium. Frequency modulation was further found to produce temporal variation in population amplitudes, promoting local and regional stability through cyclic patterns of local and regional variability. Our results highlight the importance of approaching spatial synchrony as a non-stationary phenomenon, with implications for the assessment and interpretation of spatial synchrony observed in experimental and natural systems.A predator that preys on randomly-distributed stationary energetically-equivalent small prey will probably choose its next prey to be the nearest one. But what if no prey is found within the detection range of the predator? It is hypothesized that in this case the predator will move along an arbitrary chosen direction until a prey is detected, and turn towards it. In a stochastic environment this strategy leads to a certain distribution function of distances that the predator moves between consequent prey catches. It is shown that when the detection range of the predator exceeds the average distance between prey, this distribution function becomes the nearest neighbor distribution function, whereas; wherew when the detection range is small as compared with the average distance between prey, it becomes the exponential distribution, as the distribution of distances between neighbors on a line. In the first case, the average distance between catches becomes roughly half the average distance between prey; in the second case, it becomes inversely proportional to the square of the detection range. Ocean sunfish preys on practically stationary jellyfish at depth of more than a hundred meters, in dim light. Plausibly, it can detect jellyfish only at close quarters, and hence its detection range is probably small as compared with the average distance between prey. Analysis of the tracking data from seven animals over a few days yielded many thousands of swimming segments separating consequent prey catches. Indeed, lengths of these segments were shown to have the exponential distribution. This finding not only supports the initial hypothesis of this study, but also reveals the fragility of the energetic balance of this animal. A two-fold decrease in the detection range (e.g. due to a decreased visibility) is expected to increase the average distance it moves between catches four-fold, and hence decrease its specific energy intake (the number of jellyfishes per distance moved) by the same rate.